Polymorphism of buckwheat seed storage proteins in cultivar groups, differing by their morphotype

Abstract

Aim. To study polymorphism of buckwheat seed proteins in cultivar groups of different morphotypes by the color of corolla. Methods. Electrophoresis by Laemmli method in 17.5 % separating PAAG gel. Results. Electrophoresis spectrum variants with the incidence from 1 to 76 %, common for buckwheat cultivar groups, which are different in their morphotype, were revealed. The obtained results demonstrate a particular level of heterogeneity by the revealed variants in each investigated group of buckwheat genotypes. Variants 2h and 4f, occurring with the incidence of 10 % and 8 % respectively, and specifi c for green-fl owered group of buckwheat cultivars, were also revealed. Conclusions. Different incidence of electrophoresis spectrum components in buckwheat morphotypes, different in fl ower coloring, demonstrates probable different selective value of genes, which control the synthesis of these components or their relation to the genes, closely bound to them. Taking this fact into consideration, buckwheat seed proteins may be molecular and genetic markers for identifi cation of some buckwheat morphotypes, which may be used in breeding programs.

Biopéptidos derivados de los pseudocereales: Amaranto, Quinoa, Chía y Trigo sarraceno

“…El trigo sarraceno contiene mayores proporciones de albúmina (43%) y glutelinas (15%) que de globulinas (8%) y prolaminas (3%-11%) (Zhu, 2016). Un análisis por SDS-PAGE a las proteínas comunes del trigo sarraceno reveló una masa molecular (MM) de 10, 16, 19, 24 y 30-50 kDa (Zaika, Kozub, Sozinov, Bidnyk & Karazhbey, 2019). La banda de 50 kDa se asoció a las fracciones de leguminosas 13S y de globulina similar a la vicilina 8S, en cuanto a las bandas de 10-15 kDa fueron identificadas como fracciones de albúmina (Alonso-Miravalles & O'Mahony, 2018).…”

“…Las proteínas del trigo sarraceno común y tártaro mostraron perfiles similares en los análisis de electroforesis unidimensional, pero hubo una diferencia significativa de ellos en la electroforesis bidimensional de las fracciones de proteínas entre dos razas autóctonas de trigo sarraceno común (Zhu, 2021). Esto implica que las mismas presentan distintos grados de ionización por su composición de aminoácidos, obtenida a través de un proceso de evolución y adaptación del grano (Zaika et al, 2019).…”