Aim. To establish the presence of polymorphism and differences in variants of the protein spectrum of buckwheat seed storage proteins in groups of varieties with different colors of the flower corolla. Methods. Electrophoresis using the Laemmli method in 17.5% of the separating PAAG gels, visualization and identification of the spectrum variants. Results. The common variants of the electrophoretic spectrum for all groups of buckwheat varieties and the heterogeneity of each of the studied groups of genotypes are revealed. By frequency of variants of electrophoretic spectrum between groups of varieties were detected statistically significant differences and reliable predominance of individual variants in green-flower morphotypes of buckwheat were established. Conclusions. Differences in frequency of occurrence of variants of electrophoretic spectrum in different morphotypes of buckwheat indicate the probable processes of preservation of individual adaptive alleles of genes of seed storage proteins, which occurred in populations when varieties under the pressure of breeding process.
Keywords: edible buckwheat, spare proteins, globulins, albumins, polymorphism.
Aim. To study polymorphism of buckwheat seed proteins in cultivar groups of different morphotypes by the color of corolla. Methods. Electrophoresis by Laemmli method in 17.5 % separating PAAG gel. Results. Electrophoresis spectrum variants with the incidence from 1 to 76 %, common for buckwheat cultivar groups, which are different in their morphotype, were revealed. The obtained results demonstrate a particular level of heterogeneity by the revealed variants in each investigated group of buckwheat genotypes. Variants 2h and 4f, occurring with the incidence of 10 % and 8 % respectively, and specifi c for green-fl owered group of buckwheat cultivars, were also revealed. Conclusions. Different incidence of electrophoresis spectrum components in buckwheat morphotypes, different in fl ower coloring, demonstrates probable different selective value of genes, which control the synthesis of these components or their relation to the genes, closely bound to them. Taking this fact into consideration, buckwheat seed
proteins may be molecular and genetic markers for identifi cation of some buckwheat morphotypes, which may be used in breeding programs.
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