Polymorphism of Growth-Correlated Genes Associated with Fatness and Muscle Fiber Traits in Chickens

Abstract

Thirty single nucleotide polymorphisms (SNP) and one 6-bp insertion-deletion (indel) from 8 genes of somatotropic axis were used to study the association with chicken fatness and muscle fibers. The allele frequency difference between Xinghua and White Plymouth Rock chickens was observed, and their effects on fatness and muscle fiber traits were also evaluated by linkage analyses. The G143831A (G+1705A) SNP of the growth hormone (GH) gene was related to fat width, and the G144762A (G+119A) SNP of the GH gene was significantly associated with abdominal fat pad weight, abdominal fat pad ratio, and crude fatty content of the breast muscle. The 6-bp indel of the growth hormone secretagogue receptor (GHSR) gene was significantly linked with the fat traits. The C51978309T SNP of the insulin-like factor-I (IGF-I) gene was significantly linked with the transversal area of the leg muscle fiber and transversal area of the breast muscle fiber. There was significant linkage between the insulin (INS) gene and 2 traits of the transversal area of transversal area of the leg muscle fiber and transversal area of the breast muscle fiber. Association of 30 SNP and one 6-bp indel from 8 genes of somatotropic axis with chicken fatness and muscle fiber traits was analyzed in the present study. The GH, GHSR, and leptin receptor genes were significantly related to chicken fatness. The INS and IGF-I genes were linked with muscle fiber density. Therefore, the genes of somatotropic axis not only affected chicken growth and body composition but also were associated with fatness and muscle fiber traits.

The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross

“…Polymorphism study in the g.565G>A GHR locus found that the AA genotype had the highest frequency (0.726) than the AG (0.172) and GG (0.102) in overall population. Similar result was reported in Vietnamese (0.86, Khoa et al, 2013), Noi (1.00, Khoa et al, 2013), Xinghua (1.00, Lei et al, 2007), and White Plymouth Rock (1.00, Lei et al, 2007) chickens. Ouyang et al (2008) could not detect the g.565G>A GHR polymorphism using DHPLC detection in Leghorn layer, White Recessive Rock broiler, Taihe Silkies, and Xinghua chickens.…”

“…Similarly, in the F2 population generated from White Recessive Rock and Xinghua chicken, 18 chickens with the AA genotype were found from total 451 chickens (AA genotype freq= 0.04; Nie et al, 2005). Higher GG genotype frequency was also reported by Lei et al (2007) in Xinghua (0.67) and White Plymouth Rock (1.00) chickens, Zhang et al (2007) forward and reverse primer annealing positions; bold shows Eco72I restriction sites; 499 box shows G565A SNP target (GenBank accession number: AJ506750.1). 500 maturity in semi-intensive rearing system (Yuwanta & Fujihara, 2000).…”

“…Nie et al (2005) reported that 46 SNPs in GH gene and the g.2248G>A GH (G+1705A) polymorphism in intron 3 had strong associations with growth traits in F2 White Recessive Rock and Xinghua chicken. A number of 33 SNPs in GHR gene was reported by Nie et al (2005), and the g.565G>A GHR polymorphism was reported had significant associations with fatness and muscle fiber traits in Chinese local chickens (Lei et al, 2007). Studies in other poultries reported the associations of the GH gene with growth and carcass traits in Pitalah and Kumbang Janti Indonesian ducks (Yurnalis et al, 2017), Huoyan Chinese native goose , and Japanese quail (Johari et al, 2013).…”

Association of chicken growth hormone polymorphisms with egg production

“…Compared to other animals, the intron regions of the cGH gene is highly polymorphic. Furthermore, studies using RFLP have shown that these polymorphisms are associated with abdominal fat, egg production, resistance to Marek's disease or avian leucosis, and meat yield traits (Fotouhi et al, 1993;Kuhnlein et al, 1997;Feng et al, 1997;Yan et al, 2003;Lei et al, 2007;Zhang et al, 2007;Xu et al, 2011).…”

Polymorphisms of Growth Hormone Gene in a Native Chicken Population: Association with Egg Production

“…Genotyping using PCR-RFLPs method were performed in various populations of Chinese native chickens and it was recommended that an allele present in intron 1 might be linked to laying performance (17). In other animals, growth hormone gene polymorphisms have been reported, for example a polymorphism observed in intron 3 of bovine growth hormone (bGH) gene was found to be linked to milk protein content (14). In fact, regulatory elements have been identified in the intron region of the GH gene in various animals (19).…”