Polymorphism of MC4R gene associated with feed intake, nutrient digestibility, ADG and FCR at post-weaning in Bligon goats

Latifah, L.; Maharani, Dyah; Kustantinah, K.; Hartatik, Tety

doi:10.14710/jitaa.45.3.173-180

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…The MC4R gene has been associated with productive traits in cattle [ 28 ], pigs [ 29 ], chickens [ 30 ], goats [ 31 ], and sheep [ 13 , 19 ], making it a good candidate gene to be assessed in the OPC sheep. The evaluated transition (1016G > A) is located in the 3�-untranslated region (3�-UTR) of the MC4R gene.…”

Section: Discussionmentioning

confidence: 99%

Association of the MC4R gene with growth traits and meat quality in Colombian hair sheep

Herrera,

lez,

rez

2023

J Adv Vet Anim Res

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Objective: The objective of this study was to associate the 1016G > A variant of the melanocortin-4 receptor gene with lamb’s weight, growth, and meat quality in the Colombian hair sheep breed. Materials and Methods: A total of 168 lambs, weights were measured at birth weight (BW), at weaning adjusted weaning weight (AWW), at 6 months [adjusted weight (AW180)], at slaughter adjusted slaughter weight (ASW), daily weight gain preweaning daily gain (preWDG), and postweaning daily gain (postWDG) weaning, and after slaughter, pH, texture, and cook-loss (CL) in the longissimus thoracis et lumborum (LTL) muscle according to the American Meat Science Association methodology. The 1016G > A genotypes were obtained by sequencing. Genotypic and allele frequencies, heterozygosities, and Hardy–Weinberg equilibrium (HWE) were estimated. Using a generalized linear model, the genotype and the allelic substitution effect were associated with the evaluated traits. Results: The heterozygous genotype (0.48) and G allele (0.61) were the most frequent. Heterozygosities were similar (0.47), indicating HWE. The genotype affected the BW (p < 0.05), with a higher value for the GG genotype (2.69 kg). AWW (12.75 kg), AW180 (19.67 kg), and ASW (31.21 kg) weights and daily weight gain (preWDG = 115.41 gm; postWDG = 96.16 gm) were not associated. Average pH, Warner-Bratzler shear force, and CL were 5.75 units, 49.46 N, and 32.02%, with no genotype effect. The G > A substitution only affected BW at −388 gm (p < 0.05). Conclusion: The 1016G > A variant is polymorphic and affects the BW but not other growth traits or the meat quality of the LTL muscle.

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Section: Discussionmentioning

confidence: 99%

Association of the MC4R gene with growth traits and meat quality in Colombian hair sheep

Herrera,

lez,

rez

2023

J Adv Vet Anim Res

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“…A study has been conducted to see the affiliation between polymorphism in the MC4R gene and some aspects involved in growth traits such as feed intake, feed conversion ratio, average daily gain, and weaning weight. It shows a significant association between SNP found and the weaning weight, weaning body length, digestibility matter (DM), organic matter (OM), and total digestible nutrient (TDN) of Bligon goats (Latifah et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Restriction Mapping of MC4R Gene on Bali Cattle (Bos sondaicus) as Genetic Marker for Breeding Program in Compared to Bos taurus and Bos indicus

Perdana

Hartatik²

2022

J.Tropical Biodiversity Biotechnology

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MC4R is a gene that has potential effects on growth traits such as body weight and feed intake. The usage of single nucleotide polymorphism (SNPs) in melanocortin-4 receptor (MC4R) as selection markers could help achieve effectiveness in the breeding program. This study aimed to analyze the restriction mapping based on SNPs in the MC4R gene for Bali cattle (Bos sondaicus) compared to various breeds of cattle. Partial MC4R gene was amplified using a primer (F: 5’-ACC AAT GTC AGT GAG TCC CC- 3’ and R: 5’-CTT CAT GTT GGC GCC CTG-3’) with a polymerase chain reaction (PCR) method. Genotype and allele frequencies were calculated using Chi-Square test and analyzed with Hardy–Weinberg law. Restriction enzyme was analyzed using Nebcutter V.2 to see the association between SNPs and the recognition site of restriction enzyme. The result showed four SNPs g.554 T>C, g.634 G>T, g.673 C>T, and g.742 G>A were found in the exon region. SNP g.742 G>A was found as a heterozygote genotype and the rest are SNP g.554 T>C, g.634 G>T, and g.673 C>T were found as homozygote genotypes. All SNPs were synonymous which did not change the amino acid translated. Three restriction enzymes were identified as MmeI, TspRI, and BsrI which attach to SNPs g.554 T>C, g.634 G>T, and g. 742 G>A respectively. SNPs found notably g. 742 G>A can be used as genetic markers associated with growth traits for further research on Bali cattle.

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Integrative molecular characterization and in silico analyses of caprine MC3R, MC4R, and MC5R genes

Venkatesh

Mishra

Pradhan

2022

Small Ruminant Research

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Polymorphism of MC4R gene associated with feed intake, nutrient digestibility, ADG and FCR at post-weaning in Bligon goats

Cited by 4 publications

References 10 publications

Association of the MC4R gene with growth traits and meat quality in Colombian hair sheep

Association of the MC4R gene with growth traits and meat quality in Colombian hair sheep

Restriction Mapping of <i>MC4R</i> Gene on Bali Cattle (<i>Bos sondaicus</i>) as Genetic Marker for Breeding Program in Compared to <i>Bos taurus</i> and <i>Bos indicus</i>

Integrative molecular characterization and in silico analyses of caprine MC3R, MC4R, and MC5R genes

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