Polymorphism of Methylenetetrahydrofolate Reductase (A1298c) as a Risk Factor for Osteoporosis in Post-Menopausal Indonesian Women

Abstract

Objective:The study aimed to assess the association between the A1298C polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene and risk to osteoporosis in post-menopausal Indonesian women. Methods:After ethical approval, calcaneus bone mineral density (BMD) (T-scoring) was assessed from 194 consenting post-menopausal Indonesian women by dual-energy X-ray absorptiometry, and DNA of the participants was isolated from peripheral blood samples. To determine the status of A1298C polymorphism of MTHFR, isolated DNA was polymerase chain reaction (PCR) amplified in 35 cycles, to result in PCR product sizes of 302 bp (1298A) and 275 bp (1298C). For fragment detection, the PCR product was subjected to electrophoresis on agarose gel. The results were statistically assessed for genotype and allotype comparison according to the T-score grouping by Chi-square analysis, assuming statistical significance at p<0.05. Results:The results show no significant association between the T-score (BMD) grouping and genotype (or allotype) of the tested polymorphism of MTHFR. The observed genotype distribution of the tested MTHFR polymorphism (A1298C) differs clearly from those previously reported, with AC as the dominant genotype in the Indonesian sample population of the present work. Conclusion:The MTHFR (A1298C) polymorphism is relatively infrequent in the Indonesian female population, and no significant association was observed between this polymorphism, bone mineral density, and osteoporotic status.

Association of rs235768 A>T polymorphism of the bone morphogenetic protein 2 gene on non-syndromic orofacial cleft in an Indonesian population

“…A cross sectional study of a total 128 stored DNA samples that had been extracted from peripheral blood taken from the Indonesian population using the techniques by Auerkari et al [10][11][12][13] , which were made available from the Laboratory of Oral Biology, Faculty of Dentistry University of Indonesia. This research has obtained approval from the Ethics Commission of the Faculty Dentistry University of Indonesia with the number of 77/Ethical Approval/ FKGUI/VII/2017, protocol No.…”

+17 C/G polymorphism in matrix metalloproteinase (MMP)-8 gene and its association with periodontitis

“…The study was approved by the ethics committee of the Faculty of Dentistry, Universitas Indonesia, and conducted by using samples of DNA that were stored (at -20°C) at the Oral Biology Laboratory of the Faculty of Dentistry [17][18][19]. The DNA samples were originally extracted from peripheral blood of 100 patients with periodontitis and 100 healthy individuals without periodontitis.…”