Polymorphism of Microsatellite Markers in Papaya (Carica papaya L.)

Oliveira, Eder Jorge de; V.B.O., Amorim,; Matos, Edneide Luciana Santiago; Costa, Juliana Leles; Castellen, Milene da Silva; Pádua, Juliano Gomes; Dantas, J. L. L.

doi:10.1007/s11105-010-0180-6

Cited by 39 publications

(28 citation statements)

References 57 publications

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“…Furthermore, the plants become more vulnerable to pests and disease outbreaks, which might impair the production of suitable fruits for domestic and foreign markets (OLIVEIRA et al, 2010a). On the other hand, there are several landraces selected by farmers that have been cultivated in many regions of Brazil, and several inbred lines developed by Embrapa Cassava and Fruits (CNPMF), by germplasm hybridization and selection.…”

Section: Introductionmentioning

confidence: 99%

“…In the case of papaya, some studies were conducted to characterize genetic diversity using isozymes (MORSHIDI, 1998), Random amplification of polymorphic DNA -RAPD (STILES et al, 1993;SONDUR et al, 1996;JOBIN-DÉCOR et al, 1997), Restriction Fragment Length Polymorphism (RFLP) (ARADHYA et al, 1999), Amplified Fragment Length Polymorphism (AFLP) markers (KIM et al, 2002;VAN DROOGENBROECK et al, 2002;RATCHADAPORN et al, 2007), PCR-RFLP ( VAN DROOGENBROECK et al, 2004), Inter-Simple Sequence Repeats (ISSR) (CARRASCO et al, 2009) and Simple Sequence Repeats (SSR) (OLIVEIRA et al, 2010a(OLIVEIRA et al, , 2010b. However, most of these studies are concerned with the diversity in germplasm collections without immediate commercial value for cultivar release.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of papaya genotypes using AFLP markers

et al. 2011

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-Due to the low genetic variability reported in the commercial plantations of papaya (Carica papaya L.), the objective of this study was analyze the genetic diversity of 32 genotypes including cultivars, landraces, inbred lines, and improved germplasm using the AFLP technique (Amplified Fragment Length Polymorphism). The genetic distance matrix was obtained using the Nei and Li genetic distance and clustering was performed using the unweighted pair-method with arithmetic mean (UPGMA). Using 11 combinations of EcoRI/MseI primers, 383 polymorphic bands were obtained. On average, 34.8 polymorphic bands were obtained per primer combination. Five clusters were formed. The traditional cultivar 'Sunrise' and the inbred line CMF-L30-08 were the closest genotypes, and the improved germplasm (CMF041) and landrace (CMF233) the most distant. The main papaya cultivars commercially grown in Brazil, as well as four inbred lines and three improved germplasm, were clustered together, however, were not grouped in the same branch. The genetic distance between the Sunrise and Golden cultivars was 0.329, and even arising from mutation and selection within the Sunrise variety, the Golden stores considerable genetic variability. Additional variability was observed in the inbred lines derived from papaya breeding program at Embrapa Cassava and Fruits. Index terms: papaya breeding, genetic resources, genetic diversity, Carica papaya L.. CARACTERIZAÇÃO MOLECULAR DE GENÓTIPOS DE MAMOEIRO COM USO DE MARCADORES DO TIPO AFLPRESUMO -Em virtude da baixa variabilidade genética relatada nos plantios comerciais de mamoeiro (Carica papaya L.), o objetivo deste trabalho foi analisar a divergência genética de 32 genótipos incluindo cultivares, variedades locais, linhagens e germoplasma melhorado, utilizando a técnica de AFLP (Amplified Fragment Length Polymorphism). A matriz de distâncias genéticas foi obtida com a distância de Nei e Li, e o agrupamento foi realizado utilizando o método UPGMA (unweighted pair-method with arithmetic mean). Utilizaram-se 11 combinações de iniciadores com corte das enzimas EcoRI / MseI. Foram geradas 383 bandas polimórficas, com média de 34,8 por combinação de iniciadores. O agrupamento dos dados permitiu a formação de cinco grupos, sendo a cultivar Sunrise e a linhagem CMF-L30-08 os genótipos mais relacionados, e CMF041 (germoplasma melhorado) e CMF233 (variedade local), os mais dissimilares. As cultivares de mamoeiro mais cultivadas no Brasil, bem como quatro linhagens e três acessos de germoplasma melhorado foram agrupadas, porém não no mesmo ramo. Mesmo sendo oriunda de mutação e seleção dentro de Sunrise, a cultivar Golden ainda possui considerável variabilidade genética, uma vez que a distância genética entre estas cultivares foi de 0,329. Variabilidade adicional foi observada nas linhagens do programa de melhoramento. Termos para indexação: melhoramento, recursos genéticos, diversidade genética, Carica papaya L..Rev. Bras.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of papaya genotypes using AFLP markers

et al. 2011

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“…Vários trabalhos relatam o uso de genótipos crioulos em programas de melhoramento para a ampliação da variabilidade genética e selecionar novos fenótipos e fontes de genes visando adaptação, rusticidade e resistência à doenças (Araújo e Nass, 2002;Medeiros et al, 2008). Oliveira et al (2010) avaliaram a diversidade gené-tica de genótipos locais de mamoeiro do Recôncavo Baiano e concluíram que havia variabilidade genéti-ca adicional possível de ser utilizada em programas de melhoramento. No entanto, até o momento, não existem trabalhos caracterizando genótipos crioulos (landraces) de mamoeiro quanto à resistência a doenças no Brasil.…”

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“…Oliveira et al (2010) avaliaram por marcadores microsatélite a variabilidade genética de "landraces" cultivadas por pequenos agricultores do Recôncavo Baiano e concluíram que esta pode ser usada em programas de melhoramento do mamoeiro para enriquecimento de populações melhoradas por seleção. No entanto, o conhecimento prévio das estimativas de alguns parâmetros genéticos deve ser considerado na escolha do método de seleção a ser adotado em função da característica de interesse.…”

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Patometria, parâmetros genéticos e reação de progênies de mamoeiro à pinta-preta

et al. 2012

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ResumoA resistência genética constitui-se em alternativa sustentável para o controle de doenças em culturas agrícolas, sendo esta, no entanto, dependente da avaliação de populações naturais ou tradicionais visando à ampliação da base genética. Este trabalho relata a avaliação fenotípica de 24 progênies de mamoeiro, oriundas de genótipos crioulos, cultivados por pequenos agricultores no sul do Estado do Espírito Santo. Avaliaram-se características associadas à resistência à pinta-preta, importante doença desta cultura, causada pelo fungo Asperisporium caricae. Para fins comparativos, foram incluídas uma variedade resistente ('Maradol') e uma suscetível ('Golden') à pinta-preta. Pelos parâmetros genéticos estimados, concluiu-se que na população estudada há variabilidade genética quanto à resistência à pinta-preta suficiente para indicar a seleção recorrente como método de melhoramento.Palavras-chave: Asperisporium caricae, Carica papaya, herdabilidade, resistência genética, variabilidade genética. Pathometry, genetic parameters and papaya progenies reaction to black-spot disease AbstractGenetic resistance is a sustainable alternative to control diseases in crops, being indispensable the enlargement of the genetic base to introduce new resistance genes on developing cultivars. This study reports the phenotypic evaluation of 24 papaya progenies, originated of landraces cultivated for small farmers in south of the Espírito Santo State, Brazil. Characteristics related to the resistance to black-spot disease, induced by the fungi Asperisporium caricae were evaluated. For comparison one genotype resistant ('Maradol') and one susceptible ('Golden') to black-spot disease were included on the study. Based on the genetic parameters estimated, the studied population has genetic variability for resistance to black spot diseases appropriates for use of recurrent selection as crop breeding method.

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“…In contrast, in recent years, there has been a significant increase in the application of molecular genetic methods to assess genetic relationships between papaya and related wild species (Kim et al 2002;Van Droogenbroeck et al 2004;Carrasco et al 2009;Oliveira et al, 2010b). Molecular markers offer numerous advantages over phenotype-based alternatives because the former are not confounded by environmental, pleiotropic, and epistatic effects.…”

Section: Cluster Analysismentioning

confidence: 99%

Use of morpho-agronomic traits and DNA profiling for classification of genetic diversity in papaya

Jesus

Freitas²,

Dantas³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. We examined the genetic diversity of papaya (Carica papaya) based on morpho-agronomic and molecular data. Twentyseven genotypes grown in Brazil were analyzed with 11 AFLP primer combinations, 23 ISSR markers, 22 qualitative, and 30 quantitative descriptors. For the joint analyses, we used the Gower algorithm (Joint Gower) and the average value of the individual dissimilarity matrix for each type of data (Average-Joint Gower); 359 AFLP and 52 ISSR polymorphic bands were found. Approximately 29.2 and 7.7% of the AFLP and ISSR bands, respectively, were genotype-specific and may therefore be used for papaya variety protection. Although there was a significant correlation between the qualitative and quantitative descriptor dissimilarity matrices (r = 0.43), the morpho-agronomic data were not highly correlated with the molecular data. Moreover, correlation between AFLP and ISSR dissimilarity matrices was nearly null (r = -0.01). Joint Gower analysis of all data showed high correlations, especially for AFLP markers, most likely due to the larger number of bands, generating ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 6646-6663 (2013) Germplasm characterization of papaya 6647 a strong bias in the diversity estimates. The Average-Joint Gower analysis allowed a better balance between the correlations for the continuous and the discrete variables. The results generated by clustering analysis distinguished 5 genetically distinct groups. While we found that papaya genotypes are significantly variable for many traits, we observed that Average-Joint Gower analysis allowed for genotype clustering based on the most widely used criterion for classifying papaya genotypes, which is fruit type ('Formosa' or 'Solo'). This information helps provide an accurate estimate of the genetic diversity and structure of papaya germplasm, which will be used for further breeding strategies.

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Polymorphism of Microsatellite Markers in Papaya (Carica papaya L.)

Cited by 39 publications

References 57 publications

Molecular characterization of papaya genotypes using AFLP markers

Molecular characterization of papaya genotypes using AFLP markers

Patometria, parâmetros genéticos e reação de progênies de mamoeiro à pinta-preta

Use of morpho-agronomic traits and DNA profiling for classification of genetic diversity in papaya

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