Polymorphism of the Genus <i>Isophya</i> (Orthoptera, Phaneropteridae, Barbitistinae) Revealed by RAPD

Grzywacz, Beata; Warchałowska‐Śliwa, Elżbieta

doi:10.3409/fb.56_3-4.153-157

Cited by 4 publications

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“…Yet, all of these exhibit quite a peculiar location of the studied markers. In some taxa, a significant variation of chromosome markers (Tables 2, 3) corresponds to distinct intraspecific and intrapopulation genetic and/or morphoacoustic variation [5,21,47]. Three to five FISH signal-positive chromosomes have been found in taxa of recent origin and/or populations where hybridization by secondary contact of haplotypes is suspected.…”

Section: Discussionmentioning

confidence: 99%

“…The latter case concerns the I. modesta group of species [5,19], and especially the I. rhodopensis complex, representing a few subspecies inhabiting a large area of secondary contact between possibly formerly isolated populations, where intrapopulation variation in the number of rDNA clusters is observed. This is usually connected with ongoing speciation and hybridization in zones of secondary contact [5,21,47]. …”

Section: Discussionmentioning

confidence: 99%

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A comparative study of genome organization and inferences for the systematics of two large bushcricket genera of the tribe Barbitistini (Orthoptera: Tettigoniidae: Phaneropterinae)

Grzywacz

Chobanov

Maryańska-Nadachowska

et al. 2014

BMC Evol Biol

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BackgroundPoecilimon and Isophya are the largest genera of the tribe Barbitistini and among the most systematically complicated and evolutionarily intriguing groups of Palearctic tettigoniids. We examined the genomic organization of 79 taxa with a stable chromosome number using classical (C–banding, silver and fluorochrome staining) and molecular (fluorescence in situ hybridization with 18S rDNA and (TTAGG) n telomeric probes) cytogenetic techniques. These tools were employed to establish genetic organization and differences or similarities between genera or species within the same genus and determine if cytogenetic markers can be used for identifying some taxonomic groups of species.ResultsDifferences between the karyotypes of the studied genera include some general changes in the morphology of the X chromosome in Isophya (in contrast to Poecilimon). The number of major rDNA clusters per haploid genome divided Poecilimon into two main almost equal groups (with either one or two clusters), while two rDNA clusters predominated in Isophya. In both genera, rDNA loci were preferentially located in the paracentromeric region of the autosomes and rarely in the sex chromosomes. Our results demonstrate a coincidence between the location of rDNA loci and active NORs and GC-rich heterochromatin regions. The C/DAPI/CMA3 bands observed in most Poecilimon chromosomes suggest the presence of more families of repetitive DNA sequences as compared to the heterochromatin patterns in Isophya.ConclusionsThe results show both differences and similarities in genome organization among species of the same genus and between genera. Previous views on the systematics and phylogenetic grouping of certain lineages are discussed in light of the present cytogenetic results. In some cases, variation of chromosome markers was observed to correspond with variation in other evolutionary traits, which is related to the processes of ongoing speciation and hybridization in zones of secondary contact. It was concluded that the physical mapping of rDNA sequences and heterochromatin may be used as an additional marker for understanding interspecific relationships in these groups and their routes of speciation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A comparative study of genome organization and inferences for the systematics of two large bushcricket genera of the tribe Barbitistini (Orthoptera: Tettigoniidae: Phaneropterinae)

Grzywacz

Chobanov

Maryańska-Nadachowska

et al. 2014

BMC Evol Biol

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Molecular and classical chromosomal techniques reveal diversity in bushcricket genera of Barbitistini (Orthoptera)

Warchałowska‐Śliwa

Grzywacz

Maryańska-NadachowskaA.

2013

Genome

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The cytogenetic characteristics of 17 species of bushcricket belonging to eight genera of the tribe Barbitistini were examined by fluorescence in situ hybridization with 18S rDNA and (TTAGGn) telomeric as probes and by C-banding, silver, and fluorochrome staining. These markers were used to understand chromosomal organization and evolutionary relationships between genera or species within the same genus. The number of 18S rDNA clusters per haploid genome that co-localized with active nucleolus organizer regions (NORs) ranged from one to five, with the most common pattern being the presence of one NOR-bearing chromosome. This ribosomal cistron was preferentially located in the paracentromeric region of autosomes and very rarely in the sex chromosome. The results demonstrated coincidence between the localization of major ribosomal genes and active NORs and the position of C-band and GC-rich regions. The rDNA/NOR distribution and the composition of chromosome heterochromatin proved to be good cytogenetic markers for distinguishing species and phylogenetic lines and for understanding the genomic differentiation and evolution of Barbitistini. A comparison of cytogenetic and morphological or behavioral traits suggests that morphological and behavioral specialization in this group was not followed by major karyotype modification (except for Leptophyes). However, the occurrence and distribution of different repetitive DNA sites tends to vary among the taxa.

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The bush-cricket <i>Isophya kraussii</i> (Orthoptera: Phaneropteridae): bioacoustics, distribution and description of a new subspecies from Romania

Iorgu¹,

Heller²

2013

Zootaxa

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Polymorphism of the Genus Isophya (Orthoptera, Phaneropteridae, Barbitistinae) Revealed by RAPD

Cited by 4 publications

References 20 publications

A comparative study of genome organization and inferences for the systematics of two large bushcricket genera of the tribe Barbitistini (Orthoptera: Tettigoniidae: Phaneropterinae)

A comparative study of genome organization and inferences for the systematics of two large bushcricket genera of the tribe Barbitistini (Orthoptera: Tettigoniidae: Phaneropterinae)

Molecular and classical chromosomal techniques reveal diversity in bushcricket genera of Barbitistini (Orthoptera)

The bush-cricket <i>Isophya kraussii</i> (Orthoptera: Phaneropteridae): bioacoustics, distribution and description of a new subspecies from Romania

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