Polymorphism of the prolactin gene and its association with egg production traits in native Chinese ducks

Abstract

As a candidate gene related to egg production, prolactin plays a crucial role in the reproduction of birds. In this study, polymorphism of the prolactin gene was screened in six Chinese native duck breeds (Shanma, Shaoxing, Youma, Jinyun, Jingjiang and the F 2 resource population of white Liancheng X white Kaiya) using PCR-SSCP and direct sequencing. The results showed that 12 SNPs were detected: C-213T in the 5 flanking region, A-1842G in exon 2, A-3869G in exon 4 and C-5961T in exon 5, T-295C, C-381A and A-412G in intron 1, T-2231C in intron 2, C-3949T, T-3988G and T-4009C in intron 4 and T-6052A in the 3 flanking region. The C-381A site and C-5961T site can be detected by XbaI and Pst1 PCR-RFLP, respectively. Furthermore, the C-5961T mutation results in an amino acid conversion (Cys to Arg) in the functional domain and affects the existence of a heparin binding site (L-R-R-D-S-H-K). In the F 2 resource population, association analysis demonstrated that the C-5961T polymorphism was significantly associated with egg production and egg weight, with the CC genotype associated with higher egg production and bigger egg weight than the CT genotype.

Polymorphism of prolactin gene and its association with growth and some biometrical traits in ducks

“…The expressed product of this gene is the prolactin hormone, which is a multifunctional protein hormone involved in the control of a wide variety of physiological processes in vertebrates, including osmoregulation, reproduction, immune responses (Clapp et al 1994), water and electrolyte balance, endocrinology (Bole-Feysot et al 1998). In avian species, prolactin is a crucial hormone in induction and maintenance of incubation behaviour and regulation of the follicular development (Wang et al 2011). Furthermore, it has been observed that the prolactin hormone is involved in the formation of the egg (Hazelwood 1983).…”

PCR-RFLP Analysis of D-Loop mtDNA in Indonesian Domestic Waterfowl

“…Some research reported that polymorphism analysis of mtDNA marker included ND1-6, ND4L, COX 1-3, ATP6 and ATP8 in Beijing duck in China (C. Zhang et al, 2010), prolactin gene polymorphism in purebred China duck (Shanma, Shaoxing, Youma, Jinyun, Jinjiang and interbred (F2) of white Liancheng and white Kaiya) (Wang et al, 2011) (Hao, 2009) can be done by using PCR-SSCP (Single Stranded Conformation Polymorphism) and the DNA sequencing products. The restriction profiles correspond to Wolf et al (2000).…”

Aplikasi Biomolekuler Di Dunia Perunggasan Khususnya Itik

“…Perbedaan sifat kuantitatif ini disebabkan oleh faktor genetik dan faktor lingkungan yang berupa manajemen pemeliharaan yang berbeda (Martojo, 1990 (Nenzhu et al, 2009;Wang et al, 2011;Wang et al, 2012).…”