Hakikat pembangunan berkelanjutan merupakan peningkatan kualitas hidup manusia dan menjamin keberlanjutannya. Dewasa ini, pembangunan kota hanya menitikberatkan perubahan lingkungan dan tidak membangun manusia yang menempatinya. Bahkan, perkembangan kota-kota Indonesia cenderung mengikuti mekanisme pasar, seperti permukiman di Kelurahan Terboyo Wetan Kecamatan Genuk, Semarang. Permukiman ini berkembang sejak beroperasinya zona industri Genuk pada tahun 1980-an. Awalnya, permukiman ini merupakan perkampungan nelayan yang masyarakatnya menggantungkan hidupnya pada laut. Industrialisasi besar-besaran telah mengubah kondisi kehidupan masyarakat. Untuk menelusuri lebih mendalam, penelitian ini memiliki tiga tujuan, yaitu (1) memahami kesejahteraan masyarakat, (2) memahami preferensi masyarakat terhadap kesejahteraan, dan (3) memahami seberapa besar kemampuan kesejahteraan masyarakat untuk mendukung permukiman yang berkelanjutan. Penelitian ini menggunakan metode kualitatif dan membuktikan hipotesis dengan realitas di lapangan. Temuan studi menunjukkan permukiman Terboyo Wetan rawan tidak berlanjut. Menurut penilaian objektif, dari 5 aspek kualitas hidup menurut pemahaman human settlement hanya satu aspek yang mendukung, yaitu society. Keempat aspek lainnya (man, nature, shells, dan network) tidak mendukungnya. Begitupula cara pandang warga yang bersifat antroposentris berdampak pada perilaku kurang ramah lingkungan. Ini tercermin dari makna kesejahteraan menurut warga, yaitu pemenuhan kebutuhan dasar dalam lingkup ekonomi dan belum memikirkan aspek lingkungan hidup. Bahkan, beberapa warga menganggap kondisi lingkungan yang terpuruk merupakan konsekuensi permukiman pesisir.
Susanti R, Iswari RS, Fibriana F, Indriawati. 2018. The duck cytochrome oxidase I (COI) gene: Sequence and patterns analysis for potential barcoding tool. Biodiversitas 19: 997-1003. The local duck DNA barcoding is still rarely conducted in Indonesia while DNA barcoding is extensively used as a tool of species identification and delineation tool. This study aimed to analyze the sequence and patterns of Central Javanese ducks mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Feather samples of seven breeds of native duck were collected from traditional husbandries in Central Java. The samples were employed for DNA extraction and COI gene amplification. Five haplotypes were obtained from 35 samples, i.e., haplotype A, B, C, D, and E. Also, 9 variable sites with synonym substitution was detected in four nucleotides number 55, 61, 100, and 109; whereas five synonym substitutions were identified in the nucleotides number 36, 48, 51, 66, and 756. In conclusion, this study annotates that COI mtDNA gene is essential for local ducks barcoding system.
Tomato is one of the high antioxidant potential vegetables. Nowadays, there are many techniques of tomato processings instead of fresh consumption, i.e. boiled, steamed, juiced and sauteed. Every treatment of cooking will influence the chemical compound inside the fruits and the body's nutrition intake. It is important to conduct the research on antioxidant compound especially lycopene, β-carotene, vitamin C, α-tocopherol, and its activity after processing. This research has been done using the experimental method. Tomatoes were cooked into six difference ways, and then it was extracted using the same procedure continued with antioxidant measurement. The research results showed that steaming had promoted the higher antioxidant numbers (lycopene. α-tocopherol, β-carotene and vitamin C) and higher TCA and antioxidant activities in the tomatoes than other processings. It was indicated that steaming was the best way to enhance amount, capacity and activities of antioxidants of the tomatoes.
e127 are commonly associated with neonatal diarrhea and dysentery in calves. It is important to determine minimal inhibitory concentrations(MICs) for the treatment of animals including human with E. coli infections. A total of 160 strains from 158 diarrheic cattle(1 day to 11 years old) were examined between April 1999 and December 2001 in Japan. The isolates were tested about characteristic pathogenic genes (stx 1 , stx 2 , and eaeA) by polymerase chain reaction. Pathogenic E. coli were tested in vitro for their susceptibility against 18 antimicrobial agents [ampicillin(ABPC), amoxicillin(AMPC), penicillin G(PCG), gentamycin(GM), kanamycin(KM), erythromycin(EM), tetracycline(TC), oxytetracycline(OTC), chloramphenicol(CP), bicozamycin(BCM), fosfomycin(FOM), sulfadimethoxine(SMT), oxolinic acid(OXA), nalidixic acid(NA), enrofloxacin(EFLX), norfloxacin(NFLX), colistin(CL) and trimethoprim-sulfamethoxazole(TMP-S)] using the guidelines of the Clinical and Laboratory Standards Institute(CLSI). The results showed that the E. coli strains at MIC 90 were susceptible to EFLX and GM but were resistant to EM, TC, OTC, CP, PCG, AMPC, ABPC and KM. In the case of PCG, the first peak is at the 16-32 g/ml level which is already considered a resistant value but another peak was demonstrated at a much higher MIC (>512 g/ml). The results suggested that E. coli may be still continued to develop stronger resistance.
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