Polymorphisms in Epigenetic and Meat Quality Related Genes in Fourteen Cattle Breeds and Association with Beef Quality and Carcass Traits

Abstract

Improvement for carcass traits related to beef quality is the key concern in beef production. Recent reports found that epigenetics mediates the interaction of individuals with environment and nutrition. The present study was designed to analyze the genetic effect of single nucleotide polymorphisms (SNPs) in seven epigenetic-related genes (DNMT1, DNMT3a, DNMT3b, DNMT3L, Ago1, Ago2, and HDAC5) and two meat quality candidate genes (CAPN1 and PRKAG3) on fourteen carcass traits related to beef quality in a Snow Dragon beef population, and also to identify SNPs in a total of fourteen cattle populations. Sixteen SNPs were identified and genotyped in 383 individuals sampled from the 14 cattle breeds, which included 147 samples from the Snow Dragon beef population. Data analysis showed significant association of 8 SNPs within 4 genes related to carcass and/or meat quality traits in the beef populations. SNP1 (13154420A>G) in exon 17 of DNMT1 was significantly associated with rib-eye width and lean meat color score (p<0.05). A novel SNP (SNP4, 76198537A>G) of DNMT3a was significantly associated with six beef quality traits. Those individuals with the wild-type genotype AA of DNMT3a showed an increase in carcass weight, chilled carcass weight, flank thicknesses, chuck short rib thickness, chuck short rib score and in chuck flap weight in contrast to the GG genotype. Five out of six SNPs in DNMT3b gene were significantly associated with three beef quality traits. SNP15 (45219258C>T) in CAPN1 was significantly associated with chuck short rib thickness and lean meat color score (p<0.05). The significant effect of SNP15 on lean meat color score individually and in combination with each of other 14 SNPs qualify this SNP to be used as potential marker for improving the trait. In addition, the frequencies of most wild-type alleles were higher than those of the mutant alleles in the native and foreign cattle breeds. Seven SNPs were identified in the epigenetic-related genes. The SNP15 in CAPN1 could be used as a powerful genetic marker in selection programs for beef quality improvement in the Snow Dragon Beef population.

Integrated analysis of lncRNA and mRNA expression in rainbow trout families showing variation in muscle growth and fillet quality traits

“…These two transcripts were upregulated in families with a high whiteness index (Table 2 ). Previous studies showed that the DNA methylation gene, DNMT3a , was highly expressed in skeletal muscle and significantly associated with quality traits 110 – 112 . A single transcript encoding FBXO32 (Atrogin-1_ (GSONMT00031929001) and DE lncRNA Omy400042056, located within 1.3 kb, were positively correlated (R = 0.88; Table 2 ).…”

Effect of single-nucleotide polymorphisms on the breeding value of fertility and breeding value of beef in Hungarian Simmental cattle

“…In Nelore cattle, the ASAP1 gene (ArfGAP with SH3 domain, ankyrin repeat and PH domain 1) was located on the bovine Chr 14 where there are reports of QTLs for meat production traits (Tizioto et al, 2012). Liu et al (2015) studied 14 cattle breeds, including Simmental, for 16 SNPs of eight genes. They reported possible associations in the case of the Dragon Beef population.…”

Fabp3 Polymorphism in Relation to Growth Traits in Simmental and Salers Cows

“…Genetic improvement of growth, carcass and meat quality traits is an important tool to evaluate the genetic merit of livestock. Recent reports found, that key traits such as growth and meat quality are dependent on many genes and affected by a variety of environmental factors and nutrition [Liu et al 2015, Ma et al 2015, Santiago et al 2017]. In the studies by Jahuey-Martinez et al [2016] five genes were identified as potential candidate for growth traits such as birth, weaning and yearling body weights in Charolais cattle [Jahuey-Martinez et al 2016].…”