Polymorphisms of HLA genes in Western Javanese (Indonesia): close affinities to Southeast Asian populations

Yuliwulandari, Rika; Kashiwase, Koichi; Nakajima, Humiaki; Uddin, Junaite Bin Gias; Susmiarsih, Tri Panjiasih; Sofro, Abdul Salam M.; Tokunaga, Katsushi

doi:10.1111/j.1399-0039.2008.01178.x

Cited by 50 publications

(72 citation statements)

References 36 publications

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“…Study on Chinese and Thai populations showed that the SJS/TEN for carbamazepine were associated with HLA-B*1502, but not in Caucasian and Japanese populations as B*1502 allele is extremely rare in the Caucasian and Japanese population. [30][31][32][33] The finding of various HLA-B alleles play role as risk factors in this study may also be due to the fact that these alleles were found in the Mongoloid race which comprised of Indonesian 18 , in Malaysian, 28 and in north of Chinese. 34 The finding of HLA-B*35 type allele as a protective factor for NVP allergy in this study gives an opportunity to further study to the role of CD 8 T cells in type IV drug allergy, in contrasts to the studies in other countries showing the HLA-B*35 as a strong risk factor for nevirapine allergy (OR=18,34).…”

Section: Discussionmentioning

confidence: 92%

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Association between HLA-B alleles and nevirapine-induced allergies among Indonesian HIV patients

et al. 2016

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This study aimed to investigate the association between human leukocyte antigen-B (HLA-B) alleles and nevirapine allergy in HIV patients in Indonesia. A case control study was conducted involving 147 HIV patients comprising of 50 patients with and 97 patients without nevirapine allergy as control. The HLA-B allele typing was conducted by using polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP), followed by sequencing. Bivariate analysis using Chi-square (X 2 ) test and multivariate logistic regression with significance level at p<0.05 were applied to analysis the data. Among 147 subjects, 34 with HLA-B alleles were identified. Bivariable analysis showed that HLA-B*58 allele was the most significant risk factor for the nevirapine allergy (OR = 3.67; 95% CI: 1.79 to 7.54), while HLA-B*35 allele was a protective factor (OR = 0.18; 95% CI: 0.08 to 0.42). Multivariate logistic regression analysis showed that young men and HLA-B*58 allele were the significant risk factors of nevirapine allergy (OR: 4.63; 95% CI: 2.02 to 10.61), while older women with the HLA-B*35 was able to reduce the risk of nevirapine allergy approximately 81% (OR: 0.19; 95% CI: 0.08 to 0.49). In conclusion, young male with the HLA-B*58 allele are the high risk factor for nevirapine allergy in Indonesian HIV patients. ABSTRAKPenelitian ini bertujun untuk mengkaji hubungan antara alel human leukocyte antigen-B (HLA-B) dengan kejadian alergi karena nevirapine pada pasien HIV Indonesia. Penelitian dengan rancangan kasus kontrol melibatkan 147 pasien HIV terdiri dari 50 pasien dengan alergi niverapin dan 97 pasien tidak alergi sebagai kontrol. Tipe alel HLA-B dilakukan dengan metode polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) dilanjutkan dengan skuensing. Analisis bivariate dengan uji Chi-square (X 2 ) dan regresi logistik multivariat dengan tingkat signifikan p <0.05 digunakan untuk menganalisis data. Diantara 147 subjek, 34 subjek teridentifikasi membawa alel HLA-B. Hasil analisis bivariat menunjukkan bahwa HLA-B *58 merupakan factor risiko nyata terjadinya alergi nevirapin (OR = 3,67; 95% CI: 1,79 to 7,54), sedangkan alel HLA-B *35 merupakan faktor protektif (OR = 0,18; 95% CI: 0,08 to 0,42). Analisis regresi logistik multivariat menunjukkan lelaki muda dan alel HLA-B *58 faktor risiko nyata terjadinya

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Section: Discussionmentioning

confidence: 92%

“…17 The high frequency of HLA-B among Indonesian populations has been reported in the previous study. 18 This study was performed to investigate the neveripine-induced allergies in association with HLA-B alleles among Indonesian HIV patients.…”

Section: Introductionmentioning

confidence: 99%

Association between HLA-B alleles and nevirapine-induced allergies among Indonesian HIV patients

et al. 2016

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“…Keseluruhan kasus dilaporkan sembuh setelah konsumsi CBZ dihentikan. 10,11,31 Pembahasan tersebut menimbulkan suatu pertanyaan apakah reaksi simpang obat berat berupa SSJ-NET yang dipicu pemberian karbamazepin pada populasi Indonesia berhubungan dengan HLA tertentu. Lebih lanjut, hasil penelitian tersebut dapat dimanfaatkan untuk mencegah terjadinya SSJ-NET yang dipicu pemberian karbamazepin.…”

Section: Prevalensi Ssj-net Disebabkan Karbamazepinunclassified

Association Between Carbamazepine Induced Severe Cutaneous Adverse Drug Reaction and Hla Polimorphisms

Ratnaningrum

2016

MNJ

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“…These alleles had been chosen because they have high frequency in Western Javanese (Indonesia) population (Yuliwulandari 2008). The alleles HLA-DRB1*15:02 covered 23%, HLA-DRB1*07:01 covered 37.8% and HLA-DRB1*12:02 covered13.1% of western Javanese population (Gustiananda 2011).…”

Section: Kapa2g Fast Hot Start Ready MIX (Kapabiosystem)mentioning

confidence: 99%

Cloning, Expression and Bioinformatic Analysis of Human Papillomavirus Type 52 L1 Capsid Gene from Indonesian Patient

Suhandono¹,

Ungu²,

Kristianti³

et al. 2014

Microbiol Indones

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Human papillomavirus (HPV) type 52 is the most prevalent type for causing cervical cancer in Indonesian population. Cervical cancer becomes the most common cancer suffered by Indonesian women. Prevention of HPV infection can be achieved using HPV virus-like particle (VLP) vaccine derived from L1 major capsid protein. This study aimed to clone and analyze HPV-52 L1 gene. DNA obtained from biopsy of a cervical cancer patient was amplified using specific primers designed from Asian originated HPV-52 L1 gene available in the GenBank. The isolated HPV-52 L1 gene sequence was submitted to GenBank with accession number KF225497. Expression of HPV-52 L1 gene was performed using pRSET/EmGFP expression vector. We Escherichia coli analyzed and compared the HPV-52 L1 gene expressions from recombinant BL21 (DE3) that had been E.coli induced for 3 hours with 1 mM IPTG and without induction. The protein was expressed in insoluble form. We performed the following bioinformatic analyses: construction of phlyogenetic tree, T-cell epitopes prediction and 3D proteins structure modelling. We utilized the following softwares: MEGA5 for phylogenetic tree, IEDBann for MHC prediction, CLC DNA Workbench 6.5 for hydrophobicity analysis, and PDB-Viewer Deep for 3D protein structure analysis. The phylogenetic tree which was developed based on KF225497 sequence showed that it shared a branch with Asian countries (Philippines and Thailand). The deduced amino acid sequences of the predicted epitopes that were consistent in all of the programs were 259GTLGDPVPGDLYIQGS274 and 345KKESTYKNE353. This information may be useful to design diagnostic strategies and vaccine suitable for Indonesian population.Key words: epitope, HPV-L1 52 gene, human papillomavirus L1 protein Human papillomavirus ( HPV ) tipe 52 merupakan jenis penyebab kanker serviks yang paling umum pada penduduk Indonesia, terutama kaum perempuan. Pencegahan infeksi HPV dapat dicapai dengan menggunakan vaksin ( VLP ) HPV yang berasal dari L1 protein kapsid utama. Penelitian ini bertujuan untuk virus like particle mengkloning dan menganalisa L1 gen HPV-52. DNA yang diperoleh dari biopsi pasien kanker serviks diamplifikasi menggunakan primer spesifik yang dirancang dari L1 gen HPV-52 berasal dari Asia yang tersedia di GenBank. Urutan L1 gen HPV-52 yang terisolasi didaftarkan ke GenBank dengan nomor akses [KF225497]. Ekspresi L1 gen HPV-52 dilakukan dengan menggunakan vektor ekspresi Escherichia coli pRSET/EmGFP. Kami menganalisis dan membandingkan L1 gen HPV-52 dari BL21 (DE3) rekombinan E.coli yang telah diinduksi selama 3 jam dengan 1 mM IPTG dan tanpa induksi. Protein diekspresikan dalam bentuk terlarut. Kami melakukan analisis bioinformatika: pembuatan pohon filogenetik, prediksi epitop sel T, dan modeling struktur 3D protein. Kami menggunakan software MEGA5 untuk pohon filogenetik, IEDBann untuk prediksi MHC, CLC DNA Workbench 6.5 untuk analisis hidrofobik dan PDB -Viewer Deep untuk 3D analisis struktur protein . Pohon filogenetik yang dihasilkan berdasarkan pada urutan KF225497 me...

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Polymorphisms of HLA genes in Western Javanese (Indonesia): close affinities to Southeast Asian populations

Cited by 50 publications

References 36 publications

Association between HLA-B alleles and nevirapine-induced allergies among Indonesian HIV patients

Association between HLA-B alleles and nevirapine-induced allergies among Indonesian HIV patients

Association Between Carbamazepine Induced Severe Cutaneous Adverse Drug Reaction and Hla Polimorphisms

Cloning, Expression and Bioinformatic Analysis of Human Papillomavirus Type 52 L1 Capsid Gene from Indonesian Patient

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