Polymorphisms of microRNA target genesIL12B,INSR,CCND1andIL10in gastric cancer

Abstract

AIMTo evaluate associations between miRNA target genes IL12B, INSR, CCND1 and IL10 polymorphisms and gastric cancer (GC) in European population.METHODSGene polymorphisms were analyzed in 508 controls and 474 GC patients from 3 tertiary centers in Germany, Lithuania and Latvia. Controls were patients from the out-patient departments, who were referred for upper endoscopy because of dyspeptic symptoms and had no history of previous malignancy. Gastric cancer (GC) patients had histopathological verification of gastric adenocarcinoma. Genomic DNA was extracted using salting out method from peripheral blood mononuclear cells. IL12B T>G (rs1368439), INSR T>C (rs1051690), CCND1 A>C (rs7177) and IL10 T>C (rs3024498) SNPs were genotyped by the real-time polymerase chain reaction. Associations between gene polymorphism and GC were evaluated using multiple logistic regression analysis with adjustment for sex, age and country of birth.RESULTSWe observed similar distribution of genotypes and allelic frequencies of all polymorphisms between GC patients and controls except of INSR rs1051690. The frequency of the T allele of INSR gene was significantly higher in GC patients than in controls (23.26% and 19.19% respectively, P = 0.028). CT genotype was also more prevalent in patients compared to control group (38.48% and 30.12% respectively, P < 0.021). Logistic regression analysis revealed that only one polymorphism (rs1051690 in INSR gene) was associated with increased risk of GC. Carriers of CT genotype had higher odds of GC when compared to CC genotype (OR = 1.45, 95%PI: 1.08-1.95, P = 0.01). Similar association was observed in a dominant model for INSR gene, where comparison of TT+CT vs CC genotypes showed an increased risk of GC (OR = 1.44, 95%PI: 1.08-1.90, P = 0.01). Other analyzed SNPs were not associated with the presence of GC.CONCLUSIONINSR rs1051690 SNP is associated with increased risk of GC, while polymorphisms in IL12B, CCND1 and IL10 genes are not linked with the presence of GC.

Association of Long Non-Coding RNA Polymorphisms with Gastric Cancer and Atrophic Gastritis

“…The possible role of different genetic alterations in the development of gastric cancer has been studied [ 13 , 14 , 15 ]. Recent studies found that lncRNAs single-nucleotide polymorphisms (SNPs) may be involved in the carcinogenesis of gastric cancer [ 16 , 17 ].…”

Network pharmacology-based strategy to investigate pharmacological mechanisms of Zuojinwan for treatment of gastritis

“…In addition, the expression of CCKBR is regarded as a symbol that has a close relationship with gastric canceration. IL-12β is a pro-inflammatory cytokine that is produced by various immune cells [ 86 , 87 ]. The expression of IL-12β can be remarkably increased when the inflammatory response occurs in the layer of gastric tissues [ 88 ].…”

Genetic polymorphisms in cyclin D1 are associated with risk of renal cell cancer in the Chinese population

“…When some of the SNPs were in complete linkage disequilibrium (r 2 = 1), only one SNP was selected for genotyping. Finally, we included four SNPs in CCND1 (rs1944129, rs7177, rs9344 and rs678653), which were recently found to be significantly associated with some malignancies in the Chinese population [ 31 , 32 , 38 ].…”