Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on Boer goat growth performance

Zhang, Chunyan; Liu, Yun; Xu, Deqing; Wen, Qunying; Li, Xiang; Zhang, Wenmin; Yang, Liguo

doi:10.1007/s11033-011-1071-0

Cited by 49 publications

(42 citation statements)

References 25 publications

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“…Myostatin adalah anggota dari superfamili transforming growth factor (TGF)-β dan memegang peranan penting dalam mengatur pertumbuhan otot dan kualitas daging (Zhang et al 2012). Gen myostatin telah dipetakan pada kromosom dua sapi (Grobet et al 1997) dan terdiri dari tiga exon dan dua daerah intron pada semua spesies termasuk ternak babi (AY208121), kerbau (AH013313), ikan Zebra (AY323521), ayam (AF346599) dan tikus rumah (AY204900) (Esmailizadeh et al 2008).…”

Section: Gen Myostatin Dan Mutasinyaunclassified

Myostatin Gene Expression and Its Application on Goat Breeding Programme

Batubara¹

2018

WARTAZOA

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Characteristics of double muscled growth in animals are influenced by myostatin gene (MSTN). Myostatin gene is known as a member of the growth gene's superfamily (TGF-β) which works to suppress the muscle growth. However, the presence of six mutations on MSTN cause the gene inactive, and trigger the occurrence of muscle hypertrophy. Identification of myostatin gene was conducted by molecular techniques, and the most common method is polymerase chain reaction followed by singlestrand conformation polymorphism (PCR-SSCP). Research on sheep and goat in several countries showed that there had been several variations occurred in myostatin gene but further studies are required to correlate these variations to body weight gain and other important production parameters. For goat production in Indonesia, myostatin mutations cause double muscling that can be utilised for genetic improvement in goat breeding plan to produce a new breed with high quality meat.Key words: Goat, myostatin gene, mutation, double muscle ABSTRAKKarakter pertumbuhan otot ganda pada ternak dipengaruhi oleh gen myostatin (MSTN). Gen myostatin adalah anggota dari superfamili gen pertumbuhan (Transforming Growth Factor/TGF-β) dan berfungsi menekan pertumbuhan otot. Telah diidentifikasi adanya enam mutasi pada MSTN yang menyebabkan MSTN tidak aktif dan mencetuskan terjadinya pembesaran otot (hipertropi). Identifikasi gen myostatin dilakukan dengan teknik molekuler dan metode yang paling umum digunakan adalah metode PCR dan single-strand conformation polymorphism (PCR-SSCP). Penelitian pada domba dan kambing di beberapa negara menunjukkan terdapat beberapa variasi pada gen myostatin tetapi diperlukan studi lebih lanjut untuk mencari korelasi antara variasi ini dengan pertambahan bobot badan dan parameter produksi penting lainnya. Khusus untuk ternak kambing di Indonesia, mutasi myostatin yang menyebabkan hipertropi otot dapat dimanfaatkan untuk meningkatkan sifat pertumbuhan pada pembibitan kambing untuk menghasilkan bibit unggul kambing potong baru dengan kualitas daging yang baik. Kata kunci: Kambing, gen myostatin, mutasi, otot ganda PENDAHULUANPertumbuhan dan perkembangan seekor ternak merupakan suatu proses yang dinamis dan sangat dipengaruhi oleh banyak faktor seperti faktor genetik, nutrisi, regulasi hormon, efisiensi metabolisme tubuh, respon kekebalan, status fisiologi ternak, lingkungan tempat ternak itu berada dan cara pemeliharaan serta ada tidaknya kejadian penyakit/parasit (Cronje et al. 2000). Selama pertumbuhan dan perkembangan ternak, ada banyak gen yang berperan untuk mengontrol beberapa sifat tertentu pada ternak tersebut, misalnya gen-gen pengontrol pertumbuhan dan karkas, gen-gen pengontrol reproduksi, gen-gen ketahanan penyakit, gen-gen penentu warna, postur tubuh dan lain sebagainya. Ada gen yang spesifik, yang mengontrol beberapa sifat atau karakter pada ternak tetapi ada juga gabungan beberapa gen (multigen) yang mengontrol dan menentukan sifat-sifat tertentu (Amills 2014).Banyak penelitian telah dilakukan mempelajari g...

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Section: Gen Myostatin Dan Mutasinyaunclassified

Myostatin Gene Expression and Its Application on Goat Breeding Programme

Batubara¹

2018

WARTAZOA

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“…Polymorphisms in the MSTN gene have been correlated to production traits, making it a candidate target gene to enhance livestock and fowl productivity (Lu et al, 2011;Han et al, 2012 andZhang et al, 2012). However, in many cases, the allele associated with favourable production traits is not always favourable for reproductive traits.…”

Section: Introductionmentioning

confidence: 99%

Correlating single nucleotide polymorphisms in the myostatin gene with performance traits in rabbit

2016

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The Myostatin (MSTN), or Growth and Differentiation Factor 8 (GDF8), gene has been implicated in the double muscling phenomenon, in which a series of mutations render the gene inactive and unable to properly regulate muscle fibre deposition. Single nucleotide polymorphisms (SNPs) in the MSTN gene have been correlated to production traits, making it a candidate target gene to enhance livestock and fowl productivity. This study aimed to assess any association of three SNPs in the rabbit MSTN gene (c.713T>A in exon 2, c.747+34C>T in intron 2, and c.*194A>G in 3'-untranslated region) and their combinations, with carcass, production and reproductive traits. The investigated traits included individual body weight, daily body weight gain, carcass traits and reproductive traits. The 3 SNPs were screened using PCR-restriction fragment length polymorphism (RFLP)-based analysis and the effects of the different SNP genotypes and their combinations were estimated in a rabbit population. Additionally, additive and dominance effects were estimated for significant traits. The results found no significant association between the c.713 T>A SNP and all the examined traits. Allele T at the c.747+34C>T SNP was only significantly associated (P<0.05) with increased body weight at 12 wk of age. However, for the SNP residing in the 3' untranslated region (c.*194A>G), allele G was significantly associated (P<0.05) with increased body weight and high growth rate. Genotype GG at the c.*194A>G SNP also had positive effects on most carcass traits. The estimated additive genetic effect for the c.*194A>G SNP was significant (P<0.05) with most body weight, daily gain and carcass traits. No significant association was obtained between any MSTN SNPs and reproductive traits. In the combinations analysis, regardless of the genotypes of SNPs at c.713T>A and c.747+34C>T, GG at the c.*194A>G SNP correlated with highest values in body weight and daily weight gain. In conclusion, the 'G' allele at the c.*194A>G SNP had positive effects on growth and carcass traits and so could be used as a favourable allele in planning rabbit selection. Further population-wide studies are necessary to test the association of the c.*194A>G SNP with carcass traits. We also recommend evaluation of the potential effects of the c.*194A>G SNP on MSTN gene expression.

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“…In the latest study, the polymorphisms of the MSTN gene were shown to have a significant difference in different goat breeds. It was reported that the TTTTA deletion phenomenon of the MSTN gene had emerged in different species and might be unique for goats, compared with sheep, cattle, water buffalo, domestic yak, pigs, and humans (Hadjipavlou et al, 2008;Grisolia et al, 2009;Zhang et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Myostatin (MSTN) is a member of the transforming growth factor-β superfamily, which plays an essential role in the regulation of muscle growth and meat quality (Zhang et al, 2012). The MSTN gene has 3 exons and 2 introns (Bellinge et al, 2005), and encodes a glycoprotein that is expressed widely in skeletal muscle.…”

Section: Introductionmentioning

confidence: 99%

Short Communication Polymorphisms of the myostatin gene (MSTN) and its relationship with growth traits in goat breeds

Zhang¹,

Ling²,

Wang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Mutations in the myostatin (MSTN) gene can inactivate its expression and result in a non-functional protein, which leads to dramatic muscularity and a "double-muscling" phenomenon in many species. Using gene sequencing and polymerase chain reaction-singlestrand conformation polymorphism methods, polymorphisms of the MSTN gene were investigated as a candidate marker for growth in 288 goats. The results showed 2 novel single nucleotide polymorphisms: DQ167575 g.197G>A and 345A>T. Three potential genotypes (AA, AB, and BB) of substitution 197G>A in the 5'-untranslated region were detected in the 2 breeds. The polymorphism (CC and CD) of substitution 345A>T in exon I was segregated. The genetic diversity analysis revealed that Boer goat and Anhui white goat possessed intermediate genetic diversity in the P1 and P3 loci. Significant associations between the genotypes of the P3 locus and body weight, body length, and body height were observed in Boer goat and Anhui white goat (P < 0.05). It could be inferred that the MSTN gene may be a major gene or linked to the major gene affecting the goat growth traits. The polymorphic site could be a molecular marker-assisted selection program for body weight.

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Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on Boer goat growth performance

Cited by 49 publications

References 25 publications

Myostatin Gene Expression and Its Application on Goat Breeding Programme

Myostatin Gene Expression and Its Application on Goat Breeding Programme

Correlating single nucleotide polymorphisms in the myostatin gene with performance traits in rabbit

Short Communication Polymorphisms of the myostatin gene (MSTN) and its relationship with growth traits in goat breeds

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