Polymorphisms of Pit-1 gene and its association with growth traits in chicken

Bhattacharya, T. K.; Chatterjee, R. N.; Priyanka, M.

doi:10.3382/ps.2011-01990

Cited by 17 publications

(15 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Earlier studies also revealed that the differential level of expression of MSTN gene may be attributed to variability in sequence of the promoter region (Gu et al 2004;Du et al 2005). A similar type of substitution was also observed by Bhattacharya et al 2012) in the promoter region of the Pit1 gene in poultry. In order to study the effect of these mutations on MSTN gene regulation, MSTN promoter sequence was analyzed in silico for the presence of putative transcription factor binding sites at SNPs.…”

Section: Point Mutation At the Mstn Promoter Overlaps With The Transcmentioning

confidence: 70%

SNPs in minimal promoter of myostatin (GDF-8) gene and its association with body weight in broiler chicken

Paswan

Bhattacharya

Nagaraj³

et al. 2013

Journal of Applied Animal Research

Self Cite

View full text Add to dashboard Cite

Myostatin (MSTN), a transforming growth factors β (TGF-β) family member, is an important regulator of muscle mass. While polymorphism in the coding region of MSTN is extensively studied, the genetic causes of transcriptional regulation are poorly understood. This study is targeted to identify variations in the promoter region of MSTN. This study revealed that the promoter region of the growth differentiation factor 8 (GDF-8) is highly polymorphic and the identified nucleotide variations were attributed to transition mutations in sequence. Out of three genotypes observed, the frequency of AA was significantly higher (0.63) followed by the frequencies of AB (0.28) and BB (0.09). Genotype BB had a significant effect on body weight at hatching implicating the role of the identified single nucleotide polymorphisms (SNPs) in the chicks muscle development.

show abstract

Section: Point Mutation At the Mstn Promoter Overlaps With The Transcmentioning

confidence: 70%

SNPs in minimal promoter of myostatin (GDF-8) gene and its association with body weight in broiler chicken

Paswan

Bhattacharya

Nagaraj³

et al. 2013

Journal of Applied Animal Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…A 12% native PAGE (50:1, acrylamide and bisacrylamide) with 5% glycerol was prepared to resolve the SSCP pattern following a standard protocol (Bhattacharya et al, 2012). A volume of 3 µl PCR product mixed with 15 µl formamide dye (95% formamide, 0.025% xylene cyanol, 0.025% bromophenol blue, 0.5 M EDTA) was denatured at 95°C for 5 min followed by snap cooling on ice for 15 min.…”

Section: Pcr Single-stranded Conformation Polymorphism and Sequencingmentioning

confidence: 99%

“…A volume of 50 μl blood was collected aseptically from the wing vein. Genomic DNA was isolated from blood cells following a standard protocol (Bhattacharya et al, 2012). The quality of DNA was verified by 0.8% agarose gel electrophoresis while quantity was checked by spectrophotometer.…”

Section: Sample Collection and Isolationmentioning

confidence: 99%

See 1 more Smart Citation

Polymorphism and expression of insulin-like growth factor 1 (IGF1) gene and its association with growth traits in chicken

Bhattacharya

Chatterjee

Dushyanth

et al. 2015

British Poultry Science

Self Cite

View full text Add to dashboard Cite

1. The objectives of the study were to detect polymorphism in the coding region of the IGF1 gene, explore the expression profile and estimate association with growth traits in indigenous and exotic chickens. 2. A total of 12 haplotypes were found in Cornish, control layer and Aseel breeds of chicken in which the h1 haplotype was most frequent. 3. Nucleotide substitutions among haplotypes were found at 21 positions in the IGF1 gene in which 4 substitutions resulted in non-synonymous mutations in the receptor binding domain of the IGF1 protein. 4. The haplogroup showed a significant effect on body weight at 24 and 42 d of age in the control layer line, body weight at 42 d and daily weight gain between 29 and 42 d in the control broiler line, daily weight gain between 29 and 42 d in Cornish, and body weights at 42 d as well as daily weight gain between 29 and 42 d in Aseel birds. 5. IGF1 expression varied among the breeds during embryonic and post-hatch periods. The expression among the haplogroups varied in different chicken tissues. The effect of haplogroup on myofibre number in pectoral muscle was non-significant, although there was significant variation in numbers between d 1 and d 42, and between broiler and layer lines. 6. It was concluded that the coding region of the IGF1 gene was polymorphic, expressed differentially during the pre-hatch and post-hatch periods, and haplogroups showed significant association with growth traits in chicken.

show abstract

“…A pair of primers were used for amplification of GAPDH gene, which was as an internal control. The amplification and analysis was performed according standard protocol [18].…”

Section: Realtime-pcrmentioning

confidence: 99%

Cloning, characterization and expression of myostatin (growth differentiating factor-8) gene in broiler and layer chicken (Gallus gallus)

et al. 2014

View full text Add to dashboard Cite

A study was conducted to characterize myostatin gene in broiler and layer chicken and to explore mRNA expression profile in these two varieties of chicken. The myostatin cDNAs of broiler and layer varieties were cloned and sequenced. The total length of the cDNA was 1128 bp. The differences of nucleotides between PB-1 broiler and IWI layer were C > 65 > T, C > 306 > T and C > 1094 > T while those between CB broiler and IWI layer were C > 65 > T, C > 195 > G, G > 234 > A, C > 306 > T, T > 939 > C, C > 961 > T, G > 966T and C > 1094 > T. The amino acid differences of myostatin protein between PB-1 and IWI strains were alanine > 22 > valine and proline > 365 > leucine while those between CB and IWI strains were alanine > 22 > valine, histidine > 321 > tyrosine and proline > 365 > leucine. The phylogenetic study revealed closeness of PB-1 and control broiler forming a cluster, which was also closely related to IWI layer chicken formed a separate cluster. The gene was cloned and expressed in E. coli. The gene expression profile in muscle was different between broiler and layer strains. Between two broiler strains, the pattern of expression was similar. Between IWI layer and either PB-1 or CB broilers, differences in expression was found at different time points, particularly at second, fourth and seventh weeks of age. The myostatin expression was significantly associated with body weights in chicken. It is concluded that myostatin gene sequences varied at nucleotide as well as amino acid level between broiler and layer chicken varieties and the gene also expressed differentially in these two varieties.

show abstract

Polymorphisms of Pit-1 gene and its association with growth traits in chicken

Cited by 17 publications

References 14 publications

SNPs in minimal promoter of myostatin (GDF-8) gene and its association with body weight in broiler chicken

SNPs in minimal promoter of myostatin (GDF-8) gene and its association with body weight in broiler chicken

Polymorphism and expression of insulin-like growth factor 1 (IGF1) gene and its association with growth traits in chicken

Cloning, characterization and expression of myostatin (growth differentiating factor-8) gene in broiler and layer chicken (Gallus gallus)

Contact Info

Product

Resources

About