Polymorphonuclear Leucocyte Chemotaxis in Patients with Bacterial Infections

Mowat, A G; Baum, John

doi:10.1136/bmj.3.5775.617

Cited by 56 publications

(23 citation statements)

References 12 publications

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“…During the first stages of the experimental mastitis, the chemotactic response of WBC decreased in both groups (Figure 5), which is in accord with results from other studies (3, 8,13,15). In the present study, the chemotactic response of WBC recovered more rapidly in moderately diseased cows than in severely diseased cows (Figure 5).…”

Section: Discussionsupporting

confidence: 92%

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Blood Polymorphonuclear Leukocyte Chemotaxis During Experimental Escherichia coli Bovine Mastitis

Kremer¹,

Noordhuizen-Stassen²,

Grommers³

et al. 1993

Journal of Dairy Science

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The relationship between the severity of experimental Escherichia coli mastitis and the chemotactic response of blood polymorphonuclear leukocytes was investigated before and during mastitis. Experimental E. coli mastitis was induced in 10 healthy cows by inoculation of the rear right quarters with I d cfu of E. coli. Cows were classified into two groups based on the severity of the mastitis. Bacterial growth in the inoculated quarter was used as parameter that indicated severity.Before and during experimental mastitis, the chemotactic response and the number of circulating polymorphonuclear leukocytes were greater for the moderately diseased cows than for the severely diseased cows. During the first 24 h of the experimental mastitis, the chemotactic response of polymorphonuclear leukocytes decreased in both groups. Recovery of the chemotactic response of white blood cells was more rapid in moderately diseased cows than in severely diseased cows. Possibly, the larger proportion of band neutrophils (the less chemotactically active band neutrophils) partially accounts for the lower chemotactic response of the circulating

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Section: Discussionsupporting

confidence: 92%

“…During bacterial and viral infections, in vitro KREMER chemostatic response of circulating PMNL can be depressed (1,3,8,13,15). It is not known whether the in vitro chemostatic response of PMNL during experimental E. coli mastitis is depressed or whether the chemotactic response is related to the severity of disease.…”

Section: Introductionmentioning

confidence: 99%

Blood Polymorphonuclear Leukocyte Chemotaxis During Experimental Escherichia coli Bovine Mastitis

Kremer¹,

Noordhuizen-Stassen²,

Grommers³

et al. 1993

Journal of Dairy Science

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“…The data suggest that neutrophil dysfunction may be at least partially responsible for the high incidence of bacterial and possibly fungal infections in bone marrow transplant patients, particularly those with GVHD. It has been shown that infection itself may in some instances depress the PMN chemotactic response (28)(29)(30)(31). This effect may be of particular significance in severely ill patients with toxic neutrophils (28), a finding not encountered at the time of study in our patients.…”

Section: Study Population Between November 1973 and Augustmentioning

confidence: 99%

Defective neutrophil chemotaxis in bone marrow transplant patients.

Clark¹,

Johnson²,

Klebanoff³

et al. 1976

J. Clin. Invest.

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“…More recently, increased susceptibility to infection has been reported in patients with morphologically normal neutrophils and normal or elevated leukocyte counts. Impaired functional capacity of PMNLs has been observed in studies of patients with genetically inherited syndromes (1-4), neoplastic diseases (23,24), metabolic diseases (25,26), immunologic disorders (27,28), surgical diseases (29), extensive burns (30), and severe infection (5)(6)(7)(8)(9). The extent to which this occurs and th-e nature of the underlying defects remain controversial.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, several investigators have described other syndromes of neutrophil dysfunction in which a defect is presumably "acquired" before or during infection and is occasionally reversible. Leukocytes from some acutely infected patients have demonstrated diminished responses in studies of their leukotactic, phagocytic, metabolic, and microbicidal activity, but other studies have shown these functions to be normal or increased (5)(6)(7)(8)(9)(10). The rapidly changing clinical circumstances in severely ill patients and differences in the methodology used in such studies have made it difficult to evaluate these divergent conclusions.…”

mentioning

confidence: 84%

Neutrophil function in gram-negative rod bacteremia. The interaction between phagocytic cells, infecting organisms, and humoral factors.

Weinstein¹,

Young²

1976

J. Clin. Invest.

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A B S T R A C T To assess the phagocytic and bactericidal function of neutrophils in the acute stages of gramnegative rod bacteremia, cells from 30 nonleukopenic patients were studied in a test system utilizing plasma obtained simultaneously with culture-positive blood, the autologous infecting strain, and two laboratory test strains of Staphylococcus aureus and Pseudomonas aeruginosa. Results were compared to those obtained with normal neutrophils and plasma. Patient and control plasma were simultaneously tested with each source of phagocytic cells to localize any abnormalities. Four patients had a defect against their infecting strain, 33% of the inoculum phagocytized and killed versus 80% by controls. In these cases differences were localized to the patients' plasma, as normal plasma tested with patients' cells reversed the defect. Thus, four patients had impaired opsonization when compared to normal controls, but we also observed that 11 of 30 bacteremic isolates, all Escherichia coli, showed absolute or relative resistance to phagocytosis in the patient and control assay system. No intrinsic granulocyte killing abnormalities were noted. There was poor correlation between results obtained with infecting strains compared to laboratory test organisms. We conclude that in patients without evidence of an inherited neutrophil bactericidal disorder, recurrent infection, or treatment with cytotoxic drugs, intrinsic bactericidal defects are uncommon at the onset of gram-negative bacteremia, and impaired opsonization is the most commonly encountered cause of neutrophil dysfunction.

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Polymorphonuclear Leucocyte Chemotaxis in Patients with Bacterial Infections

Cited by 56 publications

References 12 publications

Blood Polymorphonuclear Leukocyte Chemotaxis During Experimental Escherichia coli Bovine Mastitis

Blood Polymorphonuclear Leukocyte Chemotaxis During Experimental Escherichia coli Bovine Mastitis

Defective neutrophil chemotaxis in bone marrow transplant patients.

Neutrophil function in gram-negative rod bacteremia. The interaction between phagocytic cells, infecting organisms, and humoral factors.

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