Polymorphonuclear proteolytic activity and milk 
composition change

Roux, Yves Le; Laurent, François; Moussaoui, F.

doi:10.1051/vetres:2003021

Cited by 118 publications

(106 citation statements)

References 70 publications

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“…In this study, protein composition was also influenced in milk from affected udder quarters in Group 2, for which the udder quarter mean SCC was 285 000 cells/ml. Plasmin and other proteolytic enzymes, such as cathepsin, elastase and collagenase, all contribute to the degradation of caseins in milk (McSweeney et al, 1995;Bastian and Brown, 1996;Le Roux et al, 2003;. Politis et al (1989) find elevated plasmin concentrations in cow composite milk with SCC of 250 000 cells/ml.…”

Section: Forsbä Ck Lindmark-må Nsson André N and Svennersten-sjaunjamentioning

confidence: 99%

Evaluation of quality changes in udder quarter milk from cows with low-to-moderate somatic cell counts

Forsbäck

Lindmark-Månsson²,

Andrén

et al. 2010

Animal

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Much emphasis has been put on evaluating alterations in milk composition caused by clinical and subclinical mastitis. However, little is known about changes in milk composition during subclinical mastitis in individual udder quarters with a low-tomoderate increase in milk somatic cell count (SCC). This information is needed to decide whether milk from individual udder quarters with a moderate-to-high increase in milk SCC should be separated or not. The aim of this study was to determine how milk composition in separate udder quarters is affected when cow composite milk has low or moderately increased SCC levels. Udder quarter and cow composite milk samples were collected from 17 cows on one occasion. Milk yield was registered and samples were analyzed for SCC, fat, total protein, whey proteins, lactose, citric acid, non-protein nitrogen (NPN), lactoferrin, protein profile, free fatty acids (FFAs), lactate dehydrogenase (LDH), proteolysis, sodium and potassium. Bacteriological samples were collected twice from all four quarters of all cows. The cows were divided into three groups depending on their SCC at udder quarter level. The first group comprised healthy cows with four udder quarters with low SCC, , 50 000 cells/ml; composition was equal when opposite rear and front quarters were compared. In the second and the third groups, cows had one udder quarter with 101 000 cells/ml , SCC , 600 000 cells/ml and SCC . 700 000 cells/ml, respectively. The remaining udder quarters of these cows had low SCC (, 100 000 cells/ml). Despite the relatively low average cow composite SCC 5 100 000 cells/ml of Group 2, milk from affected udder quarters exhibited lower casein number, content of lactose and b-casein (b-CN), while the content of whey protein, sodium, LDH and a-lactoalbumin (a-la) were higher compared to healthy opposite quarters. In addition to these changes, milk from affected udder quarters in Group 3 also exhibited lower values of potassium and a s1 -casein (a s1 -CN) and higher values of lactoferrin when compared to milk from opposite healthy quarters. This indicates that even when the SCC in cow composite milk is low, there might exist individual quarters for which milk composition is changed and milk quality impaired.

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Section: Forsbä Ck Lindmark-må Nsson André N and Svennersten-sjaunjamentioning

confidence: 99%

Evaluation of quality changes in udder quarter milk from cows with low-to-moderate somatic cell counts

Forsbäck

Lindmark-Månsson²,

Andrén

et al. 2010

Animal

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“…Sistemas de bonificações e penalizações baseados nos resultados deste estudo, além de possivelmente acarretar em resultados significativos a curto e médio prazo, pois incentivariam o produtor a investir em cuidados, principalmente no que se refere à CCS, como a limpeza e higienização dos equipamentos de ordenha e o controle da mastite, possibilitaria que o leite brasileiro alcançasse padrões internacionais de qualidade e, ainda, possivelmente, reduzir-se-iam sérios danos à indústria de derivados lácteos, como: a coagulação e floculação ocorrida durante o processamento térmico do leite pasteurizado e do leite em pó, devido à sua menor estabilidade calórica 38 ; mudanças significativas na viscosidade e sabor do iogurte 39 , geleificação e coagulação das proteínas do leite UHT durante a estocagem, devido à atividade residual de proteases bacterianas resistentes ao tratamento térmico 40 ; alterações na fabricação de queijos, como a redução no rendimento industrial 8 ; aumento no conteúdo de água no coágulo 41 ; alterações negativas nas propriedades sensoriais 42 ; aumento do tempo para formação do coágulo 43 ; baixa taxa de enrijecimento do coágulo e defeitos de textura 43 ; e elevada perda de sólidos no soro do queijo 44 .…”

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Correlações entre contagem de células somáticas e parâmetros físico-químicos e microbiológicos de qualidade do leite

et al. 2014

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Objetivou-se avaliar as correlações entre contagem de células somáticas (CCS) e os constituintes do leite e verificar as associações das condições ambientais com a CCS. Os dados utilizados foram de 1.541 unidades produtoras de leite referentes a 15 municípios da bacia leiteira do Vale do Taquari, Rio Grande do Sul. Foram tabulados os dados de CCS, contagem bacteriana total (CBT) e composição centesimal do leite, referentes ao período de junho de 2008 a dezembro de 2011, totalizando 44.089 amostras analisadas. A temperatura ambiente apresentou correlação positiva e significativa com a CCS, enquanto a precipitação pluviométrica e a umidade relativa do ar, ausência de correlação. Os teores de gordura, proteína, minerais e sólidos totais foram diretamente correlacionados com a CCS, enquanto que os sólidos não gordurosos e a lactose apresentaram comportamento inverso. Através da análise de componentes principais (ACP), auxiliada pelo método hierárquico aglomerativo de agrupamento, os sete tratamentos presentes no estudo foram reduzidos a cinco grupos de acordo com a similaridade, permitindo constatar que amostras com CCS superiores a 400.000 até 750.000 céls mL-1, apresentam a mesma qualidade, não justificando a estratificação de intervalos nesta amplitude de variação.

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“…Despite the wide use of SCC, much effort has been invested in the search for alternative biomarkers for diagnosis of mastitis as well as for evaluation of its consequences in terms of compositional changes of the milk (Korhonen & Kaartinen, 1995). Although SCC is closely linked to changes in the biochemical composition of the milk at quarter milk level (Hamann, 2005), SCC is a less sensitive or specific indicator of milk quality in bulk tank milk (Le Roux et al 2003).…”

mentioning

confidence: 99%

Haptoglobin and serum amyloid A in relation to the somatic cell count in quarter, cow composite and bulk tank milk samples

Åkerstedt

Waller

Sternesjö

2007

Journal of Dairy Research

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Milk somatic cell count (SCC) is the gold standard in diagnosis of subclinical mastitis, and is also an important parameter in quality programmes of dairy cooperatives. As routine SCC analysis is usually restricted to central laboratories, much effort has been invested in the search for alternative biomarkers of mastitis and milk quality, including the presence in the milk of the acute phase proteins (APP), haptoglobin (Hp) and serum amyloid A (SAA). The aim of this study was to investigate relationships between Hp, SAA and SCC in quarter, cow composite, and bulk tank milk samples. Cows (n = 165), without any clinical signs of disease or abnormalities in the milk or udder, from three different dairy farms, were used. Cow composite milk samples from all cows delivering milk at the sampling occasion were taken once in each herd. In one of the farms, representative quarter milk samples (n = 103) from 26 cows were also collected. In addition, bulk tank milk samples from 96 dairy farms were included in the study. Samples were analysed for Hp, SAA and SCC, and relationships between the parameters were evaluated at quarter, cow and tank milk levels using Chi-square analysis. Milk samples were categorized according to their SCC, and the presence, or no presence, of SAA and Hp, based on the detection limits of the screening methods (0 . 3 mg/l and 1 . 0 mg/l for SAA and Hp, respectively). Hp and SAA were found in milk at quarter, cow composite and bulk tank levels. A large proportion (53%) of the animals had detectable milk concentrations of APP, and SAA was detected more frequently, and at higher concentrations than Hp, regardless of sample type. SAA was detected in as many as 82 % of the bulk tank milk samples. Significant relationships were found between Hp, SAA and SCC at quarter and cow composite milk levels, but only between SAA and SCC at bulk tank milk level. Detectable levels of APP were more common at high SCC.

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Polymorphonuclear proteolytic activity and milk composition change

Cited by 118 publications

References 70 publications

Evaluation of quality changes in udder quarter milk from cows with low-to-moderate somatic cell counts

Evaluation of quality changes in udder quarter milk from cows with low-to-moderate somatic cell counts

Correlações entre contagem de células somáticas e parâmetros físico-químicos e microbiológicos de qualidade do leite

Haptoglobin and serum amyloid A in relation to the somatic cell count in quarter, cow composite and bulk tank milk samples

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