1975
DOI: 10.1016/0014-5793(75)80903-3
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Polynucleotide‐acrylamide gel electrophoresis of soluble nucleases from tobacco leaves

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Cited by 18 publications
(3 citation statements)
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“…These would be between the phosphate residues of the immobile polyanion and the positively charged groups on the surface of the migrating enzyme. These effects probably do not seriously deter the movement of most acidic, or negatively charged, proteins towards the anode in the presence of a polynucleotide at neutral or basic pH (van Loon, 1975).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…These would be between the phosphate residues of the immobile polyanion and the positively charged groups on the surface of the migrating enzyme. These effects probably do not seriously deter the movement of most acidic, or negatively charged, proteins towards the anode in the presence of a polynucleotide at neutral or basic pH (van Loon, 1975).…”
Section: Discussionmentioning
confidence: 99%
“…In the present paper we describe a technique called polynucleotide/polyacrylamide-gel electrophoresis, in which the catalytic properties of the enzyme itself are used both to define its position within the gel and to give an indication of the nature of the catalytic activity present. Although earlier attempts have been made to locate enzyme activities within gels (Wilson, 1969(Wilson, , 1971van Loon, 1975), in general the techniques suffered from a lack of sensitivity, from denaturation of enzyme activity (Randles, 1968;Rabin & Weinberger, 1975;Rosenthal & Lacks, 1977) and from poor reproducibility.…”
Section: Vol 189mentioning
confidence: 99%
“…A superior technique involves the inclusion of high-Mr substrate within the gel. This procedure reduces the number of steps required to detect activity and requires only that the enzymes be inactive and that the substrates be immobile during electrophoresis (van Loon, 1975). However, separations achieved with this method generally have been insufficient for detailed characterization and analysis of individual enzyme activities (Zoliner et al, 1974a(Zoliner et al, ,b, 1978.…”
mentioning
confidence: 99%