Polyol dehydrogenases: intermediate role in the bioconversion of rare sugars and alcohols

Lu, Fuzhi; Xu, Wei; Zhang, Wenli; Guang, Cuie; Mu, Wanmeng

doi:10.1007/s00253-019-09980-z

Cited by 16 publications

(9 citation statements)

References 82 publications

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“…To characterize the performance of KO8S16 under high xylose concentrations, we focused on arabitol, as it was significantly more abundant in KO8S16. By the reaction of XylA, xylose is converted to xylulose, which is subsequently converted to arabitol by xylulose reduction (KEGG, EC: 1.1.1.11), 43 so the increase of arabitol in KO8S16 indicated that xylose consumption efficiency of KO8S16 was improved. In addition, we found that ornithine decreased significantly in KO8S16 compared to that in the wild-type (Figure 5b).…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Engineering of Klebsiella oxytoca for the Production of 2,3-Butanediol from High Concentration of Xylose

Jung

Jang

Son

et al. 2021

ACS Sustainable Chem. Eng.

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Klebsiella oxytoca is widely used for the biological production of 2,3-butanediol (2,3-BDO), a promising platform chemical with a broad range of applications. Here, to improve cell growth and production of 2,3-BDO under high concentration of xylose (100 g/L), we engineered K. oxytoca using an adaptive laboratory evolution and a biosensor-derived high throughput screening strategy. First, we developed a XylR-dependent xylose biosensor for the detection of intracellular xylose, and K. oxytoca containing the xylose biosensor was used for adaptive laboratory evolution in 100 g/L xylose. Cells were isolated by FACS screening, and the isolated strain (KO8S16) showed much improved cell growth with high xylose consumption rate (1.35 g/L/h) and 2,3-BDO productivity (0.53 g/L/h) compared with the wild-type strain. Through whole genome resequencing, it was revealed that a mutation in OmpR (a response regulator of osmotic stress) allowed to withstand high concentrations of xylose. Finally, fed-batch cultivation was performed by feeding high concentration of xylose, and K. oxytoca successfully produced 2,3-BDO at a concentration as high as 57.5 g/L by consuming 238.13 g/L xylose in 47 h.

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Section: ■ Experimental Sectionmentioning

confidence: 99%

Engineering of Klebsiella oxytoca for the Production of 2,3-Butanediol from High Concentration of Xylose

Jung

Jang

Son

et al. 2021

ACS Sustainable Chem. Eng.

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“…2). Galactitol is mainly used to produce D-tagatose by galactitol dehydrogenase (GDH) via C-2 oxidation (Lu et al 2019). After optimization of the induction condition, 3.16 g/L D-tagatose was obtained from 20 g/L galactitol through Gluconobacter oxydans fermentation (Manzoni et al 2001).…”

Section: Application Of Galactitolmentioning

confidence: 99%

Sugar alcohols derived from lactose: lactitol, galactitol, and sorbitol

Zhang

Chen

et al. 2020

Appl Microbiol Biotechnol

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Lactose is a common natural disaccharide composed of galactose and glucose molecules. It is mainly found in the whey, the byproduct of cheese and casein industries. As the supply of lactose far exceeds demand, a lot of lactose was discarded as the waste every year, which not only leads to resource waste, but also causes environmental pollution. Therefore, the deep processing of lactose as the feedstock has become a hot research topic. The lactose-derived sugar alcohols, including lactitol, sorbitol, and galactitol, have shown great potential applications not only in food manufacture, but also in pharmaceutical, cosmetic, and material fields. In this paper, we focus on the property, physiological effect, production, and application of the lactose-derived sugar alcohols. Key points• The deep processing of lactose as the feedstock has become a hot research topic.• The lactose-derived sugar alcohols show great application values.• Recent advances in the lactose-derived sugar alcohols are reviewed.

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“…Bioconversion technology for producing mannitol using lactic acid bacteria, yeast and fungi has been studied to increase its efficiency on industrial scale. In microorganism cells, mannitol is produced with a reduction process from fructose, catalyzed by mannitol dehydrogenase (MDH) enzyme (Gonçalves et al, 2019;Lu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Mannitol Production from Fructose by Using Resting Cells of Methylotrophic Yeasts

Suprahman

Basyar

Suryadi

2022

JFIKI

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Background: Mannitol is a polyol sugar widely used in pharmaceutical and food industries which can be produced by bioconversion. Using of resting cells and methanol as a carbon source are strategies to increase the efficiency of mannitol production by increasing NAD(P)H needed in the reduction process. Objectives: This research aimed to optimize bioconversion condition by using resting cells of methylotrophic yeasts with methanol and fructose as carbon source and substrate, respectively. Methods: Several isolates were used including Candida sp, Debaryomyces nepalensis and Debaryomyces hansenii and three species suspected to be yeast isolated from a local paddy field. The methylotrophic characteristic of the yeasts was screened by turbidometry. The optimization of fermentation condition was conducted by varying cultivation time (24-96 hours), resting cell concentration (30-140 mg/mL), fructose concentration (7.5-15%), ammonium sulphate concentration (0.25-0.75%) and aeration condition (50-80%). Quantitative analysis of the mannitol was conducted by HPLC with NH2 column and Refractive Index Detector. Results: D. hansenii showed the highest yield value in mannitol production (23.17%), followed by D. nepalensis, Isolate A and Candida sp. (6.52%, 6.50% and 4.38%, respectively). Variation of bioconversion condition using D. hansenii showed that the highest resting cell concentration (140 mg/mL) incubated for 72 hours, moderate fructose concentration (10%), the highest ammonium sulphate concentration (0.75%) and moderate aeration condition (70%) would result in the highest yield value of mannitol (60%). Conclusion: This finding showed the potency of D. hansenii in mannitol production and gave preliminary information of its optimum fermentation condition.

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Polyol dehydrogenases: intermediate role in the bioconversion of rare sugars and alcohols

Cited by 16 publications

References 82 publications

Engineering of Klebsiella oxytoca for the Production of 2,3-Butanediol from High Concentration of Xylose

Engineering of Klebsiella oxytoca for the Production of 2,3-Butanediol from High Concentration of Xylose

Sugar alcohols derived from lactose: lactitol, galactitol, and sorbitol

Mannitol Production from Fructose by Using Resting Cells of Methylotrophic Yeasts

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