Polyomaviruses BK and JC DNA infection in peripheral blood cells from blood donors

Haghighi, Malihe Fathi; Seyyedi, Noorossadat; Farhadi, Ali; Zare, Farahnaz; Kasraian, Leila; Dehbidi, Gholam Reza Refiei; Ranjbaran, Reza; Behzad-Behbahani, Abbas

doi:10.1016/j.bjid.2019.01.005

Cited by 13 publications

(8 citation statements)

References 19 publications

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“…The HPyVs DNAemia found in this study (1.25%) is slightly higher than values (0-0.6) described in studies carried out in blood banks from other countries, which use highly sensitive PCR methods [5][6][7]. In contrast, other previous research reported higher prevalence rates (22.5-44.4) [8,9]; it has been postulated that this may be either due to the size of the analysed sample, the detection method or even contamination [6]. However, prevalence rates reported for HPyVs may vary according to different geographic areas of the world [5,9].…”

Section: Discussioncontrasting

confidence: 84%

Infection by human polyomaviruses JCPyV and BKPyV in blood donors of Argentina

et al. 2023

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Background and ObjectivesA spectrum of blood‐borne infectious agents may be transmitted through transfusion of blood components from asymptomatic donors. Despite the persistence of polyomaviruses in blood cells, no studies have been conducted in Argentina to assess the risk of transfusion infection.Materials and MethodsWe investigated BKPyV and JCPyV in 720 blood donors, using polymerase chain reaction (PCR) for a region of T antigen common to both viruses. Positive T‐antigen samples were subjected to two additional PCR assays targeting the VP1 region. Viral genotypes were characterized by phylogenetic analysis.ResultsPolyomaviruses were detected in 1.25% (9/720) of the blood samples selected; JCPyV was identified in 0.97% (7/720) and BKPyV in 0.28% (2/720) of them. Phylogenetic analysis showed that the JCPyV sequences clustered with 2A genotype and Ia of BKPyV.ConclusionThis study describes for the first time the prevalence of polyomavirus DNA in blood donors of Córdoba, Argentina. The polyomavirus DNAemia in healthy populations suggests that those viruses are present in blood components eligible for transfusion. Therefore, the epidemiological surveillance of polyomavirus in blood banks might be incorporated into haemovigilance programmes, to determine the infectious risk and implement newer interventions to ensure the safety of blood supplies, if required.

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Section: Discussioncontrasting

confidence: 84%

Infection by human polyomaviruses JCPyV and BKPyV in blood donors of Argentina

et al. 2023

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“…Unlike our study, which evaluated viral infection using urine samples, some studies have assessed BKV and JCV in serum specimens. A recent study in Shiraz showed that 26.4% of healthy blood donors were positive for BKV DNA in their peripheral blood cells (20). The same investigation reported JC DNA in 18% of blood samples.…”

Section: Discussionmentioning

confidence: 89%

Higher Viral Load of Polyomavirus Type BK but not JC among Renal Transplant Recipients in Comparison to Donors

et al. 2022

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Scan to discover onlineBackground & Objective: Polyomaviruses types BK and JC and Cytomegalovirus (CMV) have been shown to be related to kidney transplantation complications. This study aimed to assess the prevalence of these viruses in patients receiving kidney transplantation. Methods: This cross-sectional study was performed on 40 kidney transplant recipients and 44 donors. Urine samples were used for the extraction of viral DNA. The prevalence of JC and BK viruses and their viral loads were determined by real-time polymerase chain reaction.Results: JC and BK viruses were identified in 31% and 92.3% of all subjects, respectively. The frequency of JC and BK cases was not statistically different between the recipient and donor groups (P>0.05). All patients in the donor group and 96.8% of the recipients were positive for CMV IgG antibody. The mean viral load of BK in donors and recipients was 4.5×10 10 and 3.3×10 11 copies, respectively. The mean viral load of JC was 8.6×10 7 copies in donors and 2.9×10 8 copies in recipients. The distribution of BKV was significantly higher in recipients than donors (P=0.001), while no difference was observed between the two studied groups for JCV. Conclusion:This study showed a relatively high prevalence of BK and JC viruria in both renal transplant donors and recipients. The viral load for BKV, but not JCV, was higher in recipients than in donors.

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“…We observed a relatively high proportion of positive JCV PCR in blood (i.e. 74.1% -20 out of 27 tested individuals), which is markedly higher than the supposed proportion of JCV viremia in healthy individ uals (ranging from 0% to 30% (Boukoum et al, 2016;Delbue et al, 2007;Haghighi et al, 2019;Rocca et al, 2015)). However, a significantly higher proportion has been noted in several disease states (e.g.…”

Section: Discussionmentioning

confidence: 56%

Predictive value of JC virus PCR in cerebrospinal fluid in the diagnosis of PML

Swinnen

Saegeman

Beuselinck

et al. 2019

Diagnostic Microbiology and Infectious Disease

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Objective: To assess the predictive value of JC virus (JCV) PCR in cerebrospinal fluid (CSF) in the diagnosis of progressive multifocal leukoencephalopathy (PML). Methods: We conducted a retrospective database query to identify patients with positive CSF JCV PCR. Clinical features, final diagnosis and quantitative PCR results were obtained. Results: A positive CSF JCV PCR had a PPV of 10.4% for the diagnosis of PML. A weakly positive PCR had a PPV of 1.6%, whereas a moderately to highly positive PCR had a PPV of 92.3%. A PPV of 0.0% was observed in immunocompetent patients and in patients without compatible clinical or radiological features. Conclusions: A false-positive CSF JCV PCR is highly prevalent in our clinical practice. This test should be reserved for patients with a clinical suspicion of PML and the quantitative result of the PCR should be taken into account when making the diagnosis of PML.

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Polyomaviruses BK and JC DNA infection in peripheral blood cells from blood donors

Cited by 13 publications

References 19 publications

Infection by human polyomaviruses JCPyV and BKPyV in blood donors of Argentina

Infection by human polyomaviruses JCPyV and BKPyV in blood donors of Argentina

Higher Viral Load of Polyomavirus Type BK but not JC among Renal Transplant Recipients in Comparison to Donors

Predictive value of JC virus PCR in cerebrospinal fluid in the diagnosis of PML

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