Polyphenol content, anti-lipase and antioxidant activity of teas made in Georgia

Gulua, L.; Nikolaishvili, Lika; Jgenti, Merab; Turmanidze, Tamar; Dzneladze, G.

doi:10.1016/j.aasci.2018.06.006

Cited by 21 publications

(20 citation statements)

References 32 publications

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“…Chemical analysis conducted in this work showed that CEE contains a concentration of total polyphenols (3475.23 mg/l) higher than that of other well-studied antioxidant natural products such as red wine [32] and green and black teas from Camellia sinensis Kuntze leaves [33]. Interestingly, a large part of these polyphenols is represented by monomeric flavonoids; in particular, hyperoside and quercetin glycosides were the most abundant constituents.…”

Section: Discussionmentioning

confidence: 64%

Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

Biagi

Noto

Corsini

et al. 2019

Oxidative Medicine and Cellular Longevity

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This study was aimed at evaluating in vitro the effects of a 75% v/v ethanolic extract of leaves of Castanea sativa Mill. (var. Bastarda Rossa, Mount Amiata, Tuscany, Italy) on ejaculated human sperm. Total polyphenols and total flavonoids contained in the extract were determined by a colorimetric assay and HPLC-DAD. The DPPH test and electrochemistry were utilized to study the antioxidant activity of the extract. Swim-up-selected sperm from 8 healthy men were treated with the C. sativa leaf extract at different dilutions (1 : 100, 1 : 200, and 1 : 500), and sperm motility was assessed following the WHO guidelines. Swim-up-selected spermatozoa were incubated with 100 μM H2O2 to induce lipid peroxidation (LPO) and with H2O2 and the leaf extract (1 : 100, 1 : 200, and 1 : 500) to test the antioxidant activity of the extract. The levels of LPO were determined by measuring malondialdehyde (MDA) concentrations. The treated samples were also analyzed by transmission electron microscopy (TEM) for ultrastructural evaluation. The chemical analysis showed that one-third ca. of the polyphenols in the C. sativa extract were made up of flavonoids, with hyperoside present in high concentration. A good antioxidant activity was demonstrated by both the DPPH test and electrochemical analysis. The C. sativa leaf extract did not decrease sperm motility at all tested dilutions. Treatment with H2O2 alone caused a significant increment in MDA levels (P=0.006993), while the treatment with H2O2 plus C. sativa extract diluted to 1 : 100 and 1 : 200 significantly reduced MDA levels (P=0.01476 and P=0.01571, respectively), with respect to H2O2 alone. TEM analysis confirmed the protective effect of the extract on damage induced by LPO, in particular that occurring at the plasma membrane level. The C. sativa leaf extract could be used in human and farm animal protocols for gamete handling, such as techniques of assisted reproduction and cryopreservation of semen, all conditions in which oxidative stress is exacerbated.

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Section: Discussionmentioning

confidence: 64%

Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

Biagi

Noto

Corsini

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Therefore, as the earliest evidence, this present study has demonstrated more activity on lipase enzyme, rather than amylase, of MM extract with IC 50 90.7 ± 4.1 µg/mL and 14.3 ± 4.1 µg orlistat equiv./mg extract. The attribution to its chemical components—phenolics, proanthocyanins, and anthocyanins—among which cyanidin-3-glucoside showed inhibitory effects on lipase enzyme higher than other glycoside forms, chlorogenic acid had good inhibitory effect to α-amylase enzyme and was superior than cyanidin glycosides [3], a proanthocyanin inhibition of both α-amylase and lipase enzymes in association to polyphenols, including catechin, was denoted [27]. However, the mechanism and inhibitory kinetics regarding lipase and amylase enzyme inhibition of MM extract remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Phenolic Profile, Antioxidant Activity, and Anti-obesogenic Bioactivity of Mao Luang Fruits (Antidesma bunius L.)

Krongyut

Sutthanut

2019

Molecules

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To investigate the anti-obesity potential of Antidesma bunius L. (MM), a Thai local fruit which is named “Mao Luang,” we have focused on the effects on pancreatic α-amylase and lipase enzyme activity and on adipocyte life cycle using the 3T3-L1 cell line as a model. In addition, the phytochemical composition and anti-oxidation potential were also analyzed using HPLC-PDA UV and colorimetric methods. The ethanolic extract of MM fruits prepared by a maceration method was used in the experiments. MM extract, yield 12.08% w/w, is composed primarily of phenolics and anthocyanins as the major phytochemicals, among which, gallic acid, catechin, anthocyanin-3-glucoside, and protocatechuic acid were initially identified. In addition, susceptibly inhibitory effects on oxidation in a DPPH assay; on lipase enzyme activity rather than amylase enzyme; and on adipocyte adipogenesis of MM were demonstrated. Interestingly, a concentration-dependent bi-modular manner of activity on adipocyte adipogenesis was discovered, whereby a significant anti-adipogenic effect was demonstrated at high concentration, whilst low concentrations of MM showed adipogenic induction. Lipolytic induction was manifested. Conclusively, the ethanolic MM extract was discovered to be a potential anti-obesity agent contributed by inhibitory effects on lipase enzyme and anti-differentiation and -adipogenesis in adipocytes which significantly correlated to the total phenolics content, as well as anti-oxidation as the mechanism of action. Nevertheless, to achieve effective application, further investigation in in vivo models should be considered.

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“…Green tea extract is a good source of phenolic compounds [ 12 ]. Chemically, green tea is a plentiful source of flavonols, flavanols, and theaflavins.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Acidic and Alkaline pH on the Physico-Mechanical Properties of Surimi-Based Edible Films Incorporated with Green Tea Extract

Munir

Javed

et al. 2020

Polymers

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The effects of green tea extract (GTE) at acidic and alkaline pH (pH 3 and 11, respectively) on the physico-mechanical, thermal, and water transmission properties of silver carp surimi-based edible films were investigated. Incorporation of GTE significantly (p < 0.05) reduced elongation at break (EAB) but improved tensile strength (TS). Significant (p < 0.05) decreases in the solubility of films and water vapor permeability (WVP) were also perceived in GTE-containing films. Transparency and color were also affected, depending upon the concentration and pH. Films prepared at an acidic pH demonstrated significantly (p < 0.05) improved WVP, transparency, solubility, and thermal stability compared to those prepared at an alkaline pH. The protein pattern of films revealed a strong interaction between phenolic compounds of GTE and surimi proteins as evidenced by the presence of a myosin heavy chain (MCH) at the top of the gel. Generally, the addition of GTE at an acidic pH had significantly (p < 0.05) greater impact on film properties than at an alkaline pH and could offer great potential for surimi protein-based edible films with improved properties.

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Polyphenol content, anti-lipase and antioxidant activity of teas made in Georgia

Cited by 21 publications

References 32 publications

Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

Phenolic Profile, Antioxidant Activity, and Anti-obesogenic Bioactivity of Mao Luang Fruits (Antidesma bunius L.)

The Effect of Acidic and Alkaline pH on the Physico-Mechanical Properties of Surimi-Based Edible Films Incorporated with Green Tea Extract

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