Polyphenol-enriched fractions from Sicilian grape pomace: HPLC–DAD analysis and antioxidant activity

Amico, Vincenzo; Chillemi, Rosa; Mangiafico, Sebastiano; Spatafora, Carmela; Tringali, Corrado

doi:10.1016/j.biortech.2007.10.037

Cited by 76 publications

(66 citation statements)

References 26 publications

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“…Several plant based extracts have been screened for investigating their antioxidant and radical scavenging activities [1], [2]. Distributions of phenolics and flavonoids in nature as antioxidants have been reported worldwide [2], [3].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activities in Methanolic Extracts of Olea Ferruginea Royle Fruits

Sharma¹,

Sharma²,

Samant³

et al. 2013

IJBBB

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Section: Introductionmentioning

confidence: 99%

Antioxidant Activities in Methanolic Extracts of Olea Ferruginea Royle Fruits

Sharma¹,

Sharma²,

Samant³

et al. 2013

IJBBB

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“…So many studies have focused on the antioxidant activities by in vitro assays. Within the antioxidant compounds, flavonoids and phenolics, with a large distribution in nature, have been studied more comprehensively (Amico et al 2008). Recently, traditional Chinese herbal medicines have been taken as the focus of investigation for natural antioxidants due to broad distribution and diversity as well as low toxicity (Lin et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai

et al. 2011

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The antioxidant potency of various extracts and fractions from the leaves and stem of Epimedium koreanum Nakai was evaluated using three esteblished methods, specifically the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radica-scavenging activity assay, the inhibitory effect on lipid peroxidation induced by Fe 2+ /ascorbate (MDA) assay and the ferric reducing power (FRP) assay. The amounts of total phenolics and total flavonoids in the extracts and fractions were determined by spectrophotometric methods and the content of icariin was determined by HPLC. The results showed that all the extracts and fractions exhibited antioxidant activities at different magnitudes of potency. The leaf extract and fractions demonstrated superior antioxidant activity in most of the assays. The decreasing order of antioxidant activities among the extracts/fractions assayed through the three methods were found to be n-BuOH fraction>ethyl acetate fraction>ethanol extract>petroleum ether fraction>-water fraction. A positive correlation was found between the amounts of total phenolics, total flavonoids and icariin and DPPH radical scavenging activity (R 2 =0.9935, 0.9944 and 0.9997, respectively) and inhibitory activity on lipid peroxidation (R 2 =0.9987, 0.9830 and 0.9886, respectively). The results suggested that Icariin was one of the main constituents contribute to the antioxidant activity of Epimedium koreanum Nakai and the n-BuOH fractions of leaf extract might be valuable antioxidant natural sources.

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“…Wine as the main product of this crop reached about 26 million tons in 2008, generating large quantities of waste including grape skins and seeds (OIV 2009). These winery byproducts are rich in high-added-value compounds including phenolic acids, flavanols and anthocyanins (Amico et al 2008;Bustamante et al 2008;de Campos et al 2008;Llobera and Canellas 2008), identified also in grapes and wine (Alonso et al 2002;Goda et al 2007;Kammerer et al 2004;Makris et al 2007;Spigno and De Faveri 2007;Shrikhande 2000). The grape byproducts either seeds or pomace, constitute a very cheap source for the extraction of antioxidants with potential health promoting and disease protective qualities (Zhang et al 2007;Louli et al 2004;Shi et al 2003), which can be used as dietary supplements, or in the production of phytochemicals, thus providing an important economic advantage (Alonso et al 2002;Negro et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Phenolic compounds, antioxidant activity and insulinotropic effect of extracts prepared from grape (Vitis vinifera L) byproducts

et al. 2013

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Grape byproducts are a rich source of phenolics having immense medicinal properties, but usually wasted from juice/wine processing industries. The present study investigates the phenolic antioxidants and the insulinotropic effect of extracts prepared from seed, skin and stems of two red wine grape cultivars: Pusa Navarang and Merlot. Pusa Navarang cultivar has shown high amounts of total phenolics (95.8 mg/ml), flavonoids (30.5 mg/ml) and flavan-3-ols (21.8 mg/ml) in seed extract and total anthocyanin (4.9 mg/ml) in its skin extract as compared to Merlot cultivar. As determined using HPLC, higher amounts of catechin hydrate (14909 mg/l) and epicatechin (9299 mg/l) were observed in its seed extract, while quercetin hydrate (5849 mg/l) was abundant in its skin extract. Similarly, ferric reducing antioxidant power (FRAP) and ABTS + . [2,2′-azinobis (3-ethylbenzothiazoline)-6-sulfonic acid] and DPPH. (1,1-diphenyl-2-picrylhy-drazyl) radicals scavenging, were higher in its seed extract, respectively being 134.8 mg/ml of Quercetin equivalent (QE), 18.7 mM of trolox equivalent (TE) and 33.5 mM of TE. Strong correlation was obtained between FRAP and total phenolics, flavonoids and flavan-3-ols contents with correlation coefficients (r 2 ) of 0.915, 0.738 and 0.838 respectively. Interestingly, there was a 2-8 fold increase in insulin secretion by isolated mice pancreatic islets at 5.5 mM and 16.5 mM glucose concentration in presence of various extracts. Overall, the seed, skin and stem byproducts of both cultivars are rich sources of phenolics and antioxidants and represent a source of new insulin secretagogues.

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Polyphenol-enriched fractions from Sicilian grape pomace: HPLC–DAD analysis and antioxidant activity

Cited by 76 publications

References 26 publications

Antioxidant Activities in Methanolic Extracts of Olea Ferruginea Royle Fruits

Antioxidant Activities in Methanolic Extracts of Olea Ferruginea Royle Fruits

Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai

Phenolic compounds, antioxidant activity and insulinotropic effect of extracts prepared from grape (Vitis vinifera L) byproducts

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