Polyphenols and diterpenoids from Plectranthus forsteri ‘Marginatus’

Kubínová, Renata; Švajdlenka, Emil; Schneiderová, Kristýna; Hanáková, Zuzana; Dall’Acqua, Stefano; Farsa, Oldřich

doi:10.1016/j.bse.2013.03.029

Cited by 16 publications

(20 citation statements)

References 15 publications

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“…The high antioxidant activity of the methanolic extracts can be attributed to the presence of higher amounts of polyphenols (latter identified by HPLC-DAD profiling) in accordance with previous studies of Plectranthus plants that revealed the extraction of high amounts of such compounds when using methanol as solvent [9,11,29].…”

Section: Resultssupporting

confidence: 87%

“…The extract concentration producing 50% inhibition of cell viability (IC 50 ) was determined for the most active extract (E5) and a value of 64.52 μg/mL was found (Figure 2B). This extract can thus be considered moderately cytotoxic [29] and was selected for further studies in other cancer cell lines, which are currently ongoing.…”

Section: Resultsmentioning

confidence: 99%

“…The HPLC-DAD fingerprinting of P. madagascariensis extracts confirmed the presence of some known compounds identified in previous studies [9,11,23], namely the phenolic compounds 1 , 2 and 3 , which were present in all of these plant extracts (E1–E9), and the abietane diterpenes 7α-acetoxy-6β-hydroxyroyleanone 4 and coleon U 5 (Figure 3), common in the organic extracts obtained using the less polar solvents acetone (E4–E5) and supercritical CO 2 (E8–E9). Thus, the higher antimicrobial and cytotoxic activity of the acetonic extracts of P. madagascariensis can be attributed to the presence of these abietane diterpenes, which have been previously isolated from other Plectranthus species and shown to possess potent antibacterial [29,30] and antitumoral [31] activities, being identified as antiproliferative agents against some cancer cell lines [32,33]. The presence of those compounds could also explain the traditional use of this plant for the treatment of respiratory conditions related to infectious agents and it is also consistent with the use of P. madagascariensis for the treatment of cutaneous wounds and scabies [3].…”

Section: Resultsmentioning

confidence: 99%

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Comparison Study of Different Extracts of Plectranthus madagascariensis, P. neochilus and the Rare P. porcatus (Lamiaceae): Chemical Characterization, Antioxidant, Antimicrobial and Cytotoxic Activities

et al. 2019

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Medicinal plants of the Plectranthus genus (Lamiaceae) are known for their ethnopharmacological relevance, mainly against infectious, dermatologic and gastrointestinal pathologies. Three Plectranthus species originated from South Africa, namely P. madagascariensis, P. neochilus and the rare P. porcatus were hereby screened for their antimicrobial and cytotoxic activities related with their known and/or potential ethnomedicinal uses. Twenty-six extracts were prepared by the combination of extraction methods (infusion, decoction, microwave-assisted, ultrasound-assisted, maceration and supercritical fluid extraction) with different polarity solvents (water, methanol, acetone and supercritical CO2). The comparison study of these extracts was elucidated through the corresponding chemical characterization and cytotoxic activity data. Therefore, the acetone extract from P. madagascariensis prepared by ultrasound extraction method revealed potent antibacterial activity against Gram-positive bacteria (1.95 < minimum inhibitory concentration (MIC) < 7.81 μg/mL), including a methicillin-resistant Staphylococcus aureus (MRSA) strain. Additionally, acetone extracts from both P. madagascariensis and P. neochilus exhibited relevant antibacterial activity against Gram-negative Klebsiella pneumonia (0.48 < MIC < 3.91 μg/mL), validating the traditional uses of such plants as anti-infectious agents. All methanolic extracts showed potent antioxidant effects at 100 μg/mL measured as their radical scavenging activity (60.8–89.0%) in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The P. madagascariensis extract obtained by maceration in acetone showed moderate cytotoxic effects in the MDA-MB-231 cell line (triple negative human breast carcinoma). The extract concentration that caused a 50% inhibition in cell viability (IC50) was 64.52 μg/mL. All extracts in this comparative study were profiled by high-performance liquid chromatography-HPLC with a diode-array detector-DAD (HPLC-DAD) and the main known bioactive components were identified in each extract, which included polyphenols (caffeic 1, chlorogenic 2 and rosmarinic 3 acids), abietane diterpenes (7α-acetoxy-6β-hydroxyroyleanone 4 and coleon U 5) and flavone glycosides (rutin 6 and naringin 7).

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Comparison Study of Different Extracts of Plectranthus madagascariensis, P. neochilus and the Rare P. porcatus (Lamiaceae): Chemical Characterization, Antioxidant, Antimicrobial and Cytotoxic Activities

et al. 2019

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“…Ramu et al (2012) avaliaram as folhas de Plectranthus mollis e identificaram a presença de compostos como ácido cafeico, quercetina e luteolina. Kubínová et al (2013) avaliaram as folhas de Plectranthus forsterie encontraram compostos como ácido cafeico, confirmando a composição encontrada no presente estudo. El-hawary et al (2012) avaliou a composição química de Plectranthus amboinicus, identificando compostos como quercetina, ácido cafeico e ácido cumárico.…”

Section: Resultsunclassified

Perfil Cromatográfico E Atividade Antioxidante Frente Aos Radicais Peroxila (Roo•), Superóxido (O2•-) E DPPH Da Folha, Flor, Ramo E Inflorescência Da Plectranthus Barbatus

Pires¹,

Dolwitsch²,

Loose³

et al. 2016

CeN

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“…Nepetoidin B (NTB, Fig. ), a phenolic compound, is extracted from Perilla frutescens (Nakanishi et al , ), Plectranthus forsteri (Kubinova et al ., ), cimicifuga racemose (Jarry et al , ), and other plants (Murata et al , ; Wang et al , ). Previous studies showed that NTB has anti‐oxidant, anti‐viral, and anti‐fungal activities, and it also inhibits xanthine oxidase (Grayer et al , ; Tsai & Lee, ).…”

Section: Introductionmentioning

confidence: 99%

Nepetoidin B, a Natural Product, Inhibits LPS‐stimulated Nitric Oxide Production via Modulation of iNOS Mediated by NF‐κB/MKP‐5 Pathways

Gao

Sun

et al. 2017

Phytotherapy Research

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Previous reports showed that nepetoidin B (NTB), a natural product isolated from many herbs, has anti-fungal and anti-bacterial effects. In this study, the antiinflammatory effect of NTB was investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The cytotoxic effect of NTB and LPS was determined by MTT assay. The nitric oxide (NO) production was detected by Griess assay. The TNF-α and IL-6 levels were determined by enzyme-linked immunosorbent assay kits. Protein expressions were tested by western blotting. The transcription activity of inducible nitric oxide synthase (iNOS) was detected by luciferase assay. Immunofluorescence assay was used to observe the visualization of NF-κB/p65 nuclear translocation. NTB and LPS showed no obvious cytotoxic effect on RAW 264.7 cells. NTB remarkably inhibited LPS-induced NO and TNF-α secretion in a concentration-dependent manner while showed no significant effect on IL-6 secretion. NTB inhibited LPS-induced iNOS protein expression and transcription activity without affecting cyclooxygenase-2. Furthermore, NTB suppressed LPS-stimulated NF-κB/p65 phosphorylation and nuclear translocation. In addition, NTB significantly inhibited LPS-induced phosphorylation of JNK1/2 and p38MAPK without affecting ERK1/2. LPS-induced inhibition of mitogen-activated protein kinase phosphatase-5 (MKP-5) was completely reversed by NTB. In conclusion, these results suggested that NTB inhibited LPS-stimulated NO production possibly via modulation of iNOS mediated by MKP-5/NF-κB pathways in RAW 264.7 cells. Copyright © 2017 John Wiley & Sons, Ltd.

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Polyphenols and diterpenoids from Plectranthus forsteri ‘Marginatus’

Cited by 16 publications

References 15 publications

Comparison Study of Different Extracts of Plectranthus madagascariensis, P. neochilus and the Rare P. porcatus (Lamiaceae): Chemical Characterization, Antioxidant, Antimicrobial and Cytotoxic Activities

Comparison Study of Different Extracts of Plectranthus madagascariensis, P. neochilus and the Rare P. porcatus (Lamiaceae): Chemical Characterization, Antioxidant, Antimicrobial and Cytotoxic Activities

Perfil Cromatográfico E Atividade Antioxidante Frente Aos Radicais Peroxila (Roo•), Superóxido (O2•-) E DPPH Da Folha, Flor, Ramo E Inflorescência Da Plectranthus Barbatus

Nepetoidin B, a Natural Product, Inhibits LPS‐stimulated Nitric Oxide Production via Modulation of iNOS Mediated by NF‐κB/MKP‐5 Pathways

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