Polyphenols extracted from red grape pomace by a surfactant based method show enhanced collagenase and elastase inhibitory activity

Abstract

BACKGROUND: The aim of this study is to separate polyphenols from grape pomace using a surfactant-based separation, colloidal gas aphrons (CGA) and to investigate their inhibitory activity against skin relevant enzymes, collagenase and elastase. Ethanolic (EE) and hot water crude extracts (HWE) were produced first and then the CGA generated using TWEEN20 were applied resulting in polyphenols enriched fractions (CGA-EE and CGA-HWE, ethanol and hot water extracts derived fractions, respectively). RESULTS:Both crude extracts inhibited the enzymes in a dose-dependent manner, however, further extraction by CGA led to fractions with higher inhibitory efficiency against collagenase. Although gallic acid was the main component of the CGA-HWE, others such as kaempferol must have contributed to its potency which was more than six times that of gallic acid. The CGA-EE was found to be about four times more efficient than its crude extract and more than six times more efficient than gallic acid for collagenase inhibition; quercetin was the major polyphenol in this fraction. CONCLUSION: It is evident that ethanol and hot water extraction processes led to different polyphenols composition and thus different inhibitory activity against collagenase and elastase. Further separation with CGA increased the inhibitory potency of both extracts against collagenase. Overall the results here showed the potential for application of CGA fractions from grape extracts in cosmetics.

Surfactant TWEEN20 provides stabilisation effect on anthocyanins extracted from red grape pomace

“…A surfactant-based separation technique, colloidal gas aphrons (CGA) has been previously studied in our group to recover various valuable bioactive compounds from 4 different feedstock such as astaxanthin (Dermiki, Bourquin, & Jauregi, 2010;Dermiki, Gordon, & Jauregi, 2009), proteins (Fuda & Jauregi, 2006;Fuda, Bhatia, Pyle, & Jauregi, 2005) and polyphenols (MohdMaidin, Michael, Oruna-Concha, & Jauregi, 2017;Spigno, Dermiki, Pastori, Casanova, & Jauregi, 2010;Spigno, Amendola, Dahmoune, & Jauregi, 2015). The type of surfactant (i.e cationic, anionic and non-ionic) determines the outer charge of the CGA, where molecules with the opposite charge will attract to the CGA resulting in their effective separation into the CGA phase.…”

Colloidal Gas Aphrons separation to obtain polyphenol rich fractions from artichoke agro-industrial discards

“…Some of the emerging extraction techniques include Colloidal Gas Aphrons (CGA) (Galanakis, 2012). The use of CGA at this stage can be advantageous as it will avoid the use of large volumes of alcoholic solvents and can lead to the recovery of polyphenols in a surfactant rich solution which could be advantageous for their formulation (MohdMaidin et al, 2017).…”

“…Moreover, CGAs have been applied to the recovery of bioactive molecules such as enzymes (Jauregi and Varley, 1999;Noble and Varley, 1999), yeast cells (Hashim et al, 2000), proteins (Fuda et al, 2004(Fuda et al, & 2005, carotenoids (Dermiki et al, 2009;Alves et al, 2006) and antioxidants (Spigno and Jauregi, 2005;Dermiki et al, 2009;Spigno et al, 2010Spigno et al, 2011MohdMaidin et al, 2017). The use of some surfactants, such as Tween, has shown protection of polyphenolic compounds from oxidation (Lin et al, 2007;Spigno et al, 2014) and enhancement in the efficiency of the delivery of polyphenols in skin formulations (Yutani et al, 2012).…”

Is Bioseparation still the limiting step in bioprocess development?

“…Finally, the research papers of this Special Issue present selected advances in the field of primary recovery, purification and bioprocess development . Examples from the primary recovery of products include: a new method to extract recombinant intracellular GFP from E. coli by using aqueous solutions of surface‐active compounds, a novel integrated bioseparation process for the in situ recovery of L‐asparaginase from fermentation broth, a stable and efficient flocculation method associated with depth filtration for continuous cell separation and an approach using ultrafiltration for whey protein separation .…”

“…Examples from the primary recovery of products include: a new method to extract recombinant intracellular GFP from E. coli by using aqueous solutions of surface‐active compounds, a novel integrated bioseparation process for the in situ recovery of L‐asparaginase from fermentation broth, a stable and efficient flocculation method associated with depth filtration for continuous cell separation and an approach using ultrafiltration for whey protein separation . The development of a high‐gradient magnetic fishing process for recovery of immunoglobulins from unclarified antiserum and the potential for application of colloidal gas aphrons fractions from grape extracts in cosmetics are nicely described as alternative techniques for product recovery. From the particular field of ATPS using ionic liquids, the primary recovery of; sinomenine from Sinomenium acutum , immunoglobulin G and levodopa are critically discussed.…”