Polyphenols from Sophora yunnanensis, and Their Inhibitory Effects on Nitric Oxide Production

Abstract

A new flavonoid, (2R,3R)-3,7,4 -trihydroxy-3 ,5 -dimethoxyflavanone, named sophorayunnanol (1), together with eight known polyphenols (2-9), were isolated from the roots of Sophora yunnanensis C. Y. MA (Leguminosae). The structure including absolute stereochemistry of 1 was determined by spectroscopic (high resolution (HR)-MS, 1D-and 2D-NMR, and circular dichroism (CD)) methods. The inhibitory activity of these compounds was examined against nitric oxide (NO) production by lipopolysaccharide (LPS) and interferon (IFN)-g g activated macrophages, RAW264.7 cells. 3,7,3 ,5 -Tetrahydroxy-4 -methoxyflavone (6) and piceatannol (9) showed potent inhibitory activity against the production of NO with IC 50 values of 14.4؎ ؎2.5 and 12.9؎ ؎1.8 m mM, respectively. This article is the first report on phytochemical study of S. yunnanensis.

Intestinal anti-inflammatory activity of red wine extract: unveiling the mechanisms in colonic epithelial cells

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