Polyphosphate-Accumulating and Denitrifying Bacteria Isolated from Anaerobic-Anoxic and Anaerobic-Aerobic Sequencing Batch Reactors

Merzouki, Mohammed; Delgenès, Jean-Philippe; Bernet, Nicolas; Moletta, R.; Benlemlih, Mohammed

doi:10.1007/pl00006776

Cited by 52 publications

(32 citation statements)

References 32 publications

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“…Bacteria of the genus Bacillus have the activity for accumulating phosphorus in the form of polyphosphates (Merzouki et al 1999), and this phosphorus is then released during intracellular respiration (Couillard and Zhu 1993). In the study reported on in this paper, however, the variations in total and phosphate phosphorus concentrations were attributable not only to the activity of the microorganisms but also to the hydrolysis and reprecipitation of suspended solids.…”

Section: Phosphorus Removalmentioning

confidence: 66%

Efficiency of aerobic biodegradation of beet molasses vinasse under non-controlled pH: conditions for betaine removal / Efektywność tlenowej biodegradacji buraczanego wywaru melasowego przy nieregulowanym pH podłoża: określenie warunków usunięcia betainy

Ryznar-Luty¹,

Cibis²,

Krzywonos³

et al. 2015

Archives of Environmental Protection

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Abstract:The aim of the study was to establish such conditions that would provide high-effi ciency aerobic biodegradation of beet molasses vinasse with a mixed culture of thermo-and mesophilic bacteria of the genus Bacillus in batch processes without controlling the pH of the medium. Particular consideration was given to the betaine removal (the main pollutant of vinasse), which accounted for as much as 37.6% of total organic carbon. Biodegradation was performed in a stirred tank reactor at 27-63°C with initial pH (pH 0 ) of 6.5 and 8.0. Effi ciency of biodegradation was expressed in terms of reduction in SCOD sum , which is a sum of SCOD (soluble chemical oxygen demand, i.e. COD determined after suspended solids separation) and theoretical COD of betaine. The values achieved at 27 and 36°C with pH 0 = 8.0 exceeded 77.7%, whereas those obtained at 36 and 45°C with pH 0 = 6.5 were higher than 83.6%. The high biodegradation effi ciency obtained in the four processes is attributable to the betaine removal by the bacterial strains used in the study. Maximal extent of reduction in SCOD sum (85.41%), BOD 5 (97.91%) and TOC (86.32%), and also the fastest rate of biodegradation (1.17 g O 2 /l•h) was achieved at 36°C and pH 0 = 8.0.

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Section: Phosphorus Removalmentioning

confidence: 66%

Efficiency of aerobic biodegradation of beet molasses vinasse under non-controlled pH: conditions for betaine removal / Efektywność tlenowej biodegradacji buraczanego wywaru melasowego przy nieregulowanym pH podłoża: określenie warunków usunięcia betainy

Ryznar-Luty¹,

Cibis²,

Krzywonos³

et al. 2015

Archives of Environmental Protection

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“…Hascoet and Florentz (10), however, reported on a simultaneous uptake and release of phosphorus under anoxic conditions in the presence of an external carbon source, while van Niel et al (27) showed that, in the presence of nitrate, phosphorus release and acetate uptake by activated sludge organisms were severely inhibited. The studies described above, as well as most other studies on denitrification and phosphate removal in wastewater treatment plants (12,21), are based on the assumption that denitrifying PAOs with the physiological characteristics summarized in Table 1 are the only PAOs present in these environments. The present study demonstrates also that heterotrophic denitrifiers like P. denitrificans exhibit the ability to synthesize polyphosphate.…”

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confidence: 99%

Atypical Polyphosphate Accumulation by the Denitrifying Bacterium Paracoccus denitrificans

Barak

Rijn

2000

Appl Environ Microbiol

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Polyphosphate accumulation by Paracoccus denitrificans was examined under aerobic, anoxic, and anaerobic conditions. Polyphosphate synthesis by this denitrifier took place with either oxygen or nitrate as the electron acceptor and in the presence of an external carbon source. Cells were capable of poly-␤-hydroxybutyrate (PHB) synthesis, but no polyphosphate was produced when PHB-rich cells were incubated under anoxic conditions in the absence of an external carbon source. By comparison of these findings to those with polyphosphateaccumulating organisms thought to be responsible for phosphate removal in activated sludge systems, it is concluded that P. denitrificans is capable of combined phosphate and nitrate removal without the need for alternating anaerobic/aerobic or anaerobic/anoxic switches. Studies on additional denitrifying isolates from a denitrifying fluidized bed reactor suggested that polyphosphate accumulation is widespread among denitrifiers.Due to environmental problems associated with phosphorus discharge, phosphorus removal has become a standard treatment practice in wastewater purification plants. Enhanced biological phosphorus removal (EBPR) is the most common biological phosphorus removal method. The method is based on enrichment of so-called polyphosphate-accumulating organisms (PAOs) through recycling of the sludge between anaerobic and aerobic zones. Under these conditions, the PAOs release phosphorus in the anaerobic zone and store phosphorus as polyphosphate in the aerobic zone. Phosphorus is subsequently removed from the process stream by harvesting a fraction of the phosphorus-rich bacterial biomass (26).Recently, it was demonstrated that, not only under aerobic conditions but also under anoxic conditions, i.e., with nitrate as the electron acceptor, some PAOs are capable of polyphosphate accumulation (3,6,12,15). Since attempts to isolate bacteria with all characteristics attributed to PAOs have failed so far, information on these organisms is based on studies with crude sludge samples and enrichment cultures obtained from EBPR plants. On the basis of these studies, the following set of metabolic properties has been attributed to PAOs (22).Under anaerobic conditions, acetate or other low-molecularweight organic compounds are converted to polyhydroxyalkanoates (PHA), polyphosphate and glycogen are degraded, and phosphate is released. Under aerobic or anoxic conditions, PHA is converted to glycogen, phosphate is assimilated, and polyphosphate is intracellularly produced. Under the latter conditions, bacterial growth and phosphate uptake are regulated by the energy released from the breakdown of PHA.We studied the phosphorus dynamics in a prototype treatment system used for removal of organic matter and inorganic nitrogen from recirculating fish culture systems. The system comprised a nitrifying filter for oxidation of ammonia to nitrate, a digestion basin for anaerobic digestion of organic matter, and a denitrifying filter for reduction of nitrate to nitrogen gas (1). It was found that m...

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“…These are denitrifying bacteria that under anaerobic conditions use nitrate as final acceptor electrons and have been found on SBR reactors converting all NO -3 in the medium to N 2 (Merzouki et al, 1999). Three others had similarity with uncultured bacterium clones isolated from the swine sludge.…”

Section: Resultsmentioning

confidence: 99%

Bacterial biodiversity from an anaerobic up flow bioreactor with ANAMMOX activity inoculated with swine sludge

Viancelli

Kunz

Esteves

et al. 2011

Braz. arch. biol. technol.

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Polyphosphate-Accumulating and Denitrifying Bacteria Isolated from Anaerobic-Anoxic and Anaerobic-Aerobic Sequencing Batch Reactors

Cited by 52 publications

References 32 publications

Efficiency of aerobic biodegradation of beet molasses vinasse under non-controlled pH: conditions for betaine removal / Efektywność tlenowej biodegradacji buraczanego wywaru melasowego przy nieregulowanym pH podłoża: określenie warunków usunięcia betainy

Efficiency of aerobic biodegradation of beet molasses vinasse under non-controlled pH: conditions for betaine removal / Efektywność tlenowej biodegradacji buraczanego wywaru melasowego przy nieregulowanym pH podłoża: określenie warunków usunięcia betainy

Atypical Polyphosphate Accumulation by the Denitrifying Bacterium Paracoccus denitrificans

Bacterial biodiversity from an anaerobic up flow bioreactor with ANAMMOX activity inoculated with swine sludge

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