Polyphyly of Xylosandrus Reitter inferred from nuclear and mitochondrial genes (Coleoptera: Curculionidae: Scolytinae)

Dole, Stephanie A.; Jordal, Bjarte H.; Cognato, Anthony I.

doi:10.1016/j.ympev.2009.11.011

Cited by 71 publications

(63 citation statements)

References 31 publications

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“…Although the partial nuclear 28S rDNA and mitochondrial 16S rDNA placed these collections in Euwallacea sp. #2, as did the partial ArgK, CAD and EF-1a sequences, the allele that was preferentially amplified in these two beetles with the conserved CO1 PCR primers (Dole et al, 2010;Supplementary Table S3) appeared to have been acquired from E. validus or an E. validus-like parent (Fig. 2).…”

Section: Tablementioning

confidence: 90%

“…Based on this phylogeny, 21 Euwallacea individuals from the three targeted species groups (E. fornicatus, E. validus and E. interjectus) were selected to represent the range of phylogenetic diversity, and they were additionally typed using portions of four more genes (Table 1): translation elongation factor (EF-1a, 373 bp alignment), arginine kinase (ArgK, 669 bp alignment), CAD protein (CAD, 469 bp alignment), and mitochondrial 16S rDNA (16S rDNA, 420 bp alignment). PCR amplification and DNA sequencing followed published protocols (Dole et al, 2010;Simon et al, 1994;Supplementary Table S3).…”

Section: Gene Samplingmentioning

confidence: 99%

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Discordant phylogenies suggest repeated host shifts in the Fusarium–Euwallacea ambrosia beetle mutualism

O’Donnell

Sink

Libeskind-Hadas

et al. 2015

Fungal Genetics and Biology

129

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The mutualism between xyleborine beetles in the genus Euwallacea (Coleoptera: Curculionidae: Scolytinae) and members of the Ambrosia Fusarium Clade (AFC) represents one of 11 known evolutionary origins of fungiculture by ambrosia beetles. Female Euwallacea beetles transport fusarial symbionts in paired mandibular mycangia from their natal gallery to woody hosts where they are cultivated in galleries as a source of food. Native to Asia, several exotic Euwallacea species were introduced into the United States and Israel within the past two decades and they now threaten urban landscapes, forests and avocado production. To assess species limits and to date the evolutionary diversification of the mutualists, we reconstructed the evolutionary histories of key representatives of the Fusarium and Euwallacea clades using maximum parsimony and maximum likelihood methods. Twelve species-level lineages, termed AF 1-12, were identified within the monophyletic AFC and seven among the Fusarium-farming Euwallacea. Bayesian diversification-time estimates placed the origin of the Euwallacea-Fusarium mutualism near the Oligocene-Miocene boundary ∼19-24 Mya. Most Euwallacea spp. appear to be associated with one species of Fusarium, but two species farmed two closely related fusaria. Euwallacea sp. #2 in Miami-Dade County, Florida cultivated Fusarium spp. AF-6 and AF-8 on avocado, and Euwallacea sp. #4 farmed Fusarium ambrosium AF-1 and Fusarium sp. AF-11 on Chinese tea in Sri Lanka. Cophylogenetic analyses indicated that the Euwallacea and Fusarium phylogenies were largely incongruent, apparently due to the beetles switching fusarial symbionts (i.e., host shifts) at least five times during the evolution of this mutualism. Three cospeciation events between Euwallacea and their AFC symbionts were detected, but randomization tests failed to reject the null hypothesis that the putative parallel cladogenesis is a stochastic pattern. Lastly, two collections of Euwallacea sp. #2 from Miami-Dade County, Florida shared an identical cytochrome oxidase subunit 1 (CO1) allele with Euwallacea validus, suggesting introgressive hybridization between these species and/or pseudogenous nature of this marker. Results of the present study highlight the importance of understanding the potential for and frequency of host-switching between Euwallacea and members of the AFC, and that these shifts may bring together more aggressive and virulent combinations of these invasive mutualists.

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Section: Tablementioning

confidence: 90%

Section: Gene Samplingmentioning

confidence: 99%

Discordant phylogenies suggest repeated host shifts in the Fusarium–Euwallacea ambrosia beetle mutualism

O’Donnell

Sink

Libeskind-Hadas

et al. 2015

Fungal Genetics and Biology

129

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“…As a final consideration, it was recently demonstrated that a limited number of genes (15)(16)(17)(18)(19)(20) can generate a phylogenetic tree highly congruent (with similar node support for several clades)…”

Section: Rutschmann Et Al (2017) Offers An Interesting and Uncommon mentioning

confidence: 99%

“…With the exception of nuclear ribosomal genes (18s and 28s rRNAs) are most markers useful for the resolution of Cenozoic divergences, showing lack of phylogenetic signal for Cretaceous time frames [10]. In the last years, a growing number of phylogenetic studies on beetles have started to include nuclear protein coding genes, especially EF-1α, CAD, ArgK, and wingless [11,16,17], which are also widely used in other insect taxa [18][19][20][21]. However, a relatively limited amount of work has been done to discover and select additional nuclear genes for beetle systematics [22,[23][24][25], and all studies to date were based on less than 10 molecular markers [26,27].…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogeny of bark and ambrosia beetles (Curculionidae: Scolytinae) based on 18 molecular markers

Pistone

Gohli

Jordal

2017

Systematic Entomology

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“…En este sentido, Chang et al (2013) identificaron 32 especies del género Xyleborus al desarrollar códigos de barras mediante una PCR dirigida a dos regiones del gen mitocondrial Citocromo Oxidasa subunidad I (CO1). También se han empleado técnicas moleculares para definir la filogenia de Xylosandrus (Dole et al 2010), y se ha hecho uso de la secuenciación de proteínas de la subunidad grande del ribosoma (LSU rADN), para la determinación de los hongos asociados con X. glabratus (Harrington et al 2010).…”

Section: Introductionunclassified

Estandarización y validación de la prueba de pcr anidada para el diagnóstico de especies del género xyleborus (coleoptera: curculionidae: scolytinae)

Sosa-Castillo

Reyna

Arenas

et al. 2017

AZM

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En este estudio se estandarizó y validó la técnica de PCR anidada para la detección rápida, sensible y confiable de especies del género Xyleborus mediante el uso de los “primers” externos CI-J-2183 y TL2-N-3014 e internos J2210 y N2739, que amplifican una banda de 500 pb de la región del gen mitocondrial Citocromo Oxidasa subunidad 1(CO1). Asimismo, se realizó la extracción de ADN de 26 ejemplares de Xyleborus con el kit Qiagen DNeasy® mericom Food (DMF), no reportado previamente su uso para su aplicación en insectos, que resultó en ADN suficiente y de alta calidad para reacciones de amplificación por PCR. El método permitió procesar un solo insecto por extracción, y obtener material genético de muestras conservadas en alcohol de hasta ocho años de antigüedad. El límite de detección se definió hasta una concentración de 780 pg/μl. Se optimizóla PCR en un volumen final de 15 μL sin comprometer calidad de la amplificación. La técnica estandarizada permitió la obtención de ADN de calidad, lo que aseguró alta reproducibilidad y sensibilidad en la detección de especies de Xyleborus y la secuenciación parcial del gen CO1 para las siete especies estudiadas; las secuencias consenso fueron analizadas por homología y depositadas en el GenBank.

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Polyphyly of Xylosandrus Reitter inferred from nuclear and mitochondrial genes (Coleoptera: Curculionidae: Scolytinae)

Cited by 71 publications

References 31 publications

Discordant phylogenies suggest repeated host shifts in the Fusarium–Euwallacea ambrosia beetle mutualism

Discordant phylogenies suggest repeated host shifts in the Fusarium–Euwallacea ambrosia beetle mutualism

Molecular phylogeny of bark and ambrosia beetles (Curculionidae: Scolytinae) based on 18 molecular markers

Estandarización y validación de la prueba de pcr anidada para el diagnóstico de especies del género xyleborus (coleoptera: curculionidae: scolytinae)

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