Polyploid Abnormalities in Day 3 and Day 5 Merino Sheep Embryos

Murray, John E.; Nancarrow, C. D.; Scaramuzzi, R. J.; Cognie, Y.

doi:10.1071/bi9880157

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…After measurement, all embryos were cultured in a solution of colchicine and processed for chromosome analysis (Murray et al, 1985(Murray et al, , 1986a). The chromosome data from the day 2 embryos have been reported separately (Murray et al, 1988).…”

Section: Measurement Of Embryos and Blastocystsmentioning

confidence: 99%

Reproductive wastage in the androstenedione-immune ewe

Scaramuzzi¹,

Nancarrow²,

Murray³

et al. 1993

Reproduction

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An experiment was carried out using 320 adult Merino ewes to examine the effects of immunization against an androstenedione human serum albumin conjugate (Fecundin) on ovulation rate, fertilization rate and embryo viability at days 2, 9 and 13-14 after fertilization. The ovulation rate of immunized ewes (2.19 +/- 0.06) was greater (P < 0.001) than that of control ewes (1.43 +/- 0.04). The recovery rate of embryos or of unfertilized oocytes on day 2 was reduced in immunized ewes, but fertilization rate of recovered oocytes was unaffected by immunization. The mean number of normal embryos per ewe pregnant (prolificacy) was higher and the proportion of ewes pregnant (fertility) was lower in immunized than in the control ewes. The distribution of the number of cells per embryo showed no differences in developmental age over the period of sampling, the majority of embryos at this time being at the two- to four-cell stage of development. At day 9 of pregnancy, blastocyst recovery rates were lower in immunized than in control ewes. About 90% of blastocysts recovered were developing normally in control ewes compared with 64% in immunized ewes. The majority of blastocysts recovered on day 9 had hatched from the zona pellucida prior to recovery (mean values were 94.2% and 87.8% for control and immunized groups, respectively). In control ewes single blastocysts were larger than twin blastocysts, but for the immunized ewes this difference was not significant. Both single blastocysts (P < 0.01) and twin blastocysts (P < 0.05) from immunized ewes were smaller than those from control ewes.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Measurement Of Embryos and Blastocystsmentioning

confidence: 99%

Reproductive wastage in the androstenedione-immune ewe

Scaramuzzi¹,

Nancarrow²,

Murray³

et al. 1993

Reproduction

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“…Chromosomal imbalances are considered a significant cause of embryonic death in mammals. Studies on preimplantation stages showed chromosomal anomalies in 2% of mice (Maudlin and Fraser, 1978) and hamster (Sengoku and Dukelow, 1988), in 10% of pig (Mc-Feely, 1967), in 6-11% of sheep (Long and Williams, 1980;Murray et al, 1988;Boland et al, 1986), and i n 20% of human embryos (Angel1 et al, 1983;. In the rabbit, up to 17% of preimplantation embryos showed an abnormal chromosomal composition (for references, see Discussion).…”

Section: Introductionmentioning

confidence: 99%

High frequency of DNA ploidy abnormalities in preimplantation embryos of the rabbit

Schumacher

Fischer

Αγοραστός³

et al. 1993

Molecular Reproduction Devel

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The DNA ploidy of Feulgen-stained cell nuclei of in vivo preimplantation rabbit embryos was assayed by cytophotometry. DNA ploidy abnormalities were detected in single-cell nuclei readings by the criterion of > or = 5C DNA. These hypermodal DNA contents are referred as to DNA aneuploidy. Two, 4 and 6 days old rabbit embryos, all of normal gross morphology, were investigated. The incidence of embryos with DNA ploidy abnormalities increased from 17% in 2-day-old cleavage stages to 51% in 6-day-old expanded blastocysts. All these embryos were mosaics and the percentage of DNA aneuploid nuclei per embryo did not usually exceed 9%. Fifteen percent of the expanded blastocysts, however, contained up to 23% abnormal nuclei. Throughout the embryonic stages studied, the DNA content of abnormal nuclei was remarkably constant and averaged 5.8C. DNA aneuploid and euploid blastocysts did not differ in size. A maternal FSH treatment did not influence the DNA ploidy. This is the first report on the DNA ploidy pattern in preimplantation rabbit embryos. Our results indicate that DNA aneuploidy of single blastomeres is common in this species and occurs more often than generally assumed. The embryonic viability does not seem to be affected by the presence of DNA aneuploid blastomeres supporting earlier findings that a limited number of abnormal blastomeres is compatible with normal preimplantation development.

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