Polyploidy induced in the American oyster, Crassostrea virginica, with cytochalasin B

Stanley, Jon G.; Allen, Standish K.; Hidu, Herbert

doi:10.1016/0044-8486(81)90002-8

Cited by 155 publications

(55 citation statements)

References 15 publications

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“…Chromosome set manipulation in oyster species has been a popular subject since the practice began (Stanley et al, 1981), with numerous methods of inducing polyploidy (CB, 6-DMAP, caffeine, heat shock) developed and honed. There are quite a few reports on tetraploid induction in shellfish species (cf.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Evaluation of cytochalasin B and 6-dimethylaminopurine for tetraploidy induction in the Eastern oyster, Crassostrea virginica

Peachey

Allen

2016

Aquaculture

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Please cite this article as: Peachey, Brittany L., Allen Jr., Standish K., Evaluation of cytochalasin B and 6-dimethylaminopurine for tetraploidy induction in the Eastern oyster, Crassostrea virginica, Aquaculture (2015), AbstractCytochalasin B (CB) has been used to induce tetraploidy in oysters since the practice began in 1993. However, CB is toxic and presents health risks to hatchery workers who administer the treatment. 6-dimethylaminopurine (6-DMAP) is also an effective cytokinetic inhibitor, and does not carry the health risks of CB. We examined the relative effectiveness of 6-DMAP vs CB for producing tetraploids in the Eastern oyster (Crassostrea virginica). Survival and yield of tetraploids varied widely among the 15 experiments. Larvae resulting from 6-DMAP treatment had higher survival in 11 of the 14 trials on day two and day six/ seven. For yield of tetraploids, 10 of 13 6-DMAP treatments had higher proportions of tetraploids on day two and at the second sampling -day six, seven, or nine -7 of 10 had higher proportions of tetraploids. Tetraploid spat were obtained from the majority of surviving cultures. Based on these results, 6-DMAP can effectively replace CB for inducing polyploidy in C. virginica, and probably other Crassostrea spp., due to the success of the treatment, the ease of application, and the reduction in health risk to hatchery workers. This study set the precedent for the use of 6-DMAP on C. virginica and A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 2 established a new procedure for inducing tetraploids using triploid eggs. It might be possible to refine the treatment to further optimize yield of tetraploids.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Evaluation of cytochalasin B and 6-dimethylaminopurine for tetraploidy induction in the Eastern oyster, Crassostrea virginica

Peachey

Allen

2016

Aquaculture

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“…Moreover, it has been found that high rates of triploidy can be obtained by temperature treatments (e.g., Varadaraj and Pandian 1988;da Silva et al 2007), pressure shocks (e.g., Streisinger et al 1981;Gillet et al 2001) or chemical treatments (e.g., Stanley et al 1981;Utting and Child 1994). One of the main objectives of inducing triploidy in fish is to produce sterile fish that are compatible with commercial farming and fisheries management objectives (Peruzzi et al 2005).…”

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confidence: 98%

Effect of triploidization on juvenile African catfish (Clarias gariepinus)

et al. 2009

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Triploid African catfish (Clarias gariepinus) produced through cold shock 3 min post fertilization were compared to diploid offspring of the same parentage at 66 days of age. Triploid fish were significantly shorter (11%) and weighed less (18%) than diploids but showed no significant difference in mortality or cannibalism, which can be an important source of losses under aquaculture conditions. Erythrocytes of triploid fish were more oval shaped than the normal spherical shape of diploid erythrocytes, were significantly larger (38%) and had nuclei that were significantly larger (25%) than observed in diploid fish. Erythrocyte morphological characteristics show potential as an indicator for the detection of triploidy in African catfish.

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“…The relative effectiveness of the three treatments probably relates to mechanisms of action, e.g., inhibition of microfilament formation [CB (Schatten and Schatten,198 I)] or spindlelkinetochore attachment [heat, caffeine (Zinkowski et al, 1989)l. All have been used successfully to inhibit polar body formation in other bivalve molluscs (Durand et al, 1990;Gosling and Nolan, 1989;Longo, 1972;Obata and Nemoto, 1984;Stanley et al, 1981). The dose of CB used for this study was lower than that typically used by other investigators (0.5 pglml vs 1 -10 pglml).…”

Section: %Ing and Suppression Of Meiotic Eventsmentioning

confidence: 93%

Parthenogenetic development of dwarf surf dam,Mulinia lateralis, oocytes treated with polar body suppressing agents

Scarpa

Wada

Allen

1992

Invertebrate Reproduction & Development

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Parthenogenesis following oocyte activation has been observed in a number of marine invertebrates, but the fate of parthenogenesis in bivalve mollusc embryos is unclear. We used the dwarf surf clam, Mulinia lateralis, to examine parthenogenetic development of KC1-activated oocytes using the polar body suppressing agents caffeine and heat or cytochalasin B. Development was followed by epifluorescence microscopy and flowcytometric analysis using the DNA-specific fluorochrome DAPI. All agents suppressed polar body formation to some degree, putatively increasing the ploidy level and retaining a meiotic centrosome in the zygote; but the zygotes failed to develop normally. Failure of the zygotes to develop suggests that the meiotic centrosome is incapable of participating in mitosis in bivalves.

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Polyploidy induced in the American oyster, Crassostrea virginica, with cytochalasin B

Cited by 155 publications

References 15 publications

Evaluation of cytochalasin B and 6-dimethylaminopurine for tetraploidy induction in the Eastern oyster, Crassostrea virginica

Evaluation of cytochalasin B and 6-dimethylaminopurine for tetraploidy induction in the Eastern oyster, Crassostrea virginica

Effect of triploidization on juvenile African catfish (Clarias gariepinus)

Parthenogenetic development of dwarf surf dam,Mulinia lateralis, oocytes treated with polar body suppressing agents

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