Polypyrrole-Au Nanoparticles Composite as Suitable Platform for DNA Biosensor with Electrochemical Impedance Spectroscopy Detection

Electrically conductive polypyrrole-zirconium(IV) phosphate (PPy-ZrP) cation exchange nanocomposites have been synthesized for the first time by in situ chemical oxidative polymerization of pyrrole in the presence of zirconium(IV) phosphate (ZrP). Fourier Transform Infra-red spectroscopy (FTIR), field emission scanning electron microscopy, transmission electron microscopy, X-ray diffraction, thermogravimetric analysis, differential thermal analysis, derivative thermogravimetry and elemental analysis were used to characterize PPy-ZrP cation exchange nanocomposite. The composite showed good ion-exchange capacity (1.60 meq g -1 ), DC electrical conductivity (0.33 S cm -1 ) and isothermal stability in terms of DC electrical conductivity retention under ambient condition up to 100°C. PPy-ZrP cation exchangenanocomposite-based sensor was fabricated for the detection of ammonia vapours of aqueous ammonia. The resistivity of the nanocomposites increases on exposure to high-concentration ammonia vapours at room temperature (25°C). The rate of reaction for ammonia vapour-sensing on PPy-ZrP was observed as second order.

show abstract

“…are synthesized and used for ammonia sensing [16][17][18], DNA bio sensing, in super capacitor electrode, etc. [17].…”

Section: Introductionmentioning

confidence: 99%

“…are synthesized and used for ammonia sensing [16][17][18], DNA bio sensing, in super capacitor electrode, etc. [17]. However, conducting polymer-based ion exchangers with polyvalent sites have been poorly reported in the field of gas sensing [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

DC electrical conductivity and rate of ammonia vapour-sensing performance of synthetic polypyrrole–zirconium(IV) phosphate cation exchange nanocomposite

2017

View full text Add to dashboard Cite

show abstract

“…Mikolaj et al demonstrated a smooth film of polypyrrole with non‐aggregated AuNPs for the detection of DNA. The modification of electrode with this composite layer allowed for the deposition of a higher number of DNA probes (up to two orders of magnitude) in comparison with the traditional thiol self‐assembled monolayers . De et al fabricated a platform for biosensing of three different biomolecules viz., glucose, DNA, and protein by immobilizing glucose oxidase, single‐stranded DNA, and Lamin A antibody, respectively by using polyaniline nanowire decorated with gold nanoparticles.…”

Section: Intrinsically Conductive Polymer Composites For Biosensing Amentioning

confidence: 99%

Progress in the Development of Intrinsically Conducting Polymer Composites as Biosensors

Prajapati

Kandasubramanian

2019

Macro Chemistry & Physics

105

View full text Add to dashboard Cite

Biosensors are analytical devices which find extensive applications in fields such as the food industry, defense sector, environmental monitoring, and in clinical diagnosis. Similarly, intrinsically conducting polymers (ICPs) and their composites have lured immense interest in bio‐sensing due to their various attributes like compatibility with biological molecules, efficient electron transfer upon biochemical reactions, loading of bio‐reagent, and immobilization of biomolecules. Further, they are proficient in sensing diverse biological species and compounds like glucose (detection limit ≈0.18 nm), DNA (≈10 pm), cholesterol (≈1 µm), aptamer (≈0.8 pm), and also cancer cells (≈5 pm mL−1) making them a potential candidate for biological sensing functions. ICPs and their composites have been extensively exploited by researchers in the field of biosensors owing to these peculiarities; however, no consolidated literature on the usage of conducting polymer composites for biosensing functions is available. This review extensively elucidates on ICP composites and doped conjugated polymers for biosensing functions of copious biological species. In addition, a brief overview is provided on various forms of biosensors, their sensing mechanisms, and various methods of immobilizing biological species along with the life cycle assessment of biosensors for various biosensing applications, and their cost analysis.

show abstract

“…37−41 It is found that DNA molecules attached to Au nanoparticles acquire enhanced sensitivity and more capability for the hybridization process. 42 Au nanoparticles allow also fast and direct electron transfer between a wide range of electroactive species and electrode materials. Additionally, light-scattering properties and extremely large enhancement ability of the local electromagnetic field enables Au nanoparticles to be used as signal amplification tags in diverse biosensors.…”

Section: ■ Introductionmentioning

confidence: 99%

Enhanced Interactions of Amino Acids and Nucleic Acid Bases with Bare Black Phosphorene Monolayer Mediated by Coadsorbed Species

et al. 2019

View full text Add to dashboard Cite

In this paper, we characterize amino acids and nucleic acid bases (nucleobases), such as glutamine, histidine, tyrosine, adenine, guanine, cytosine, and thymine, and examine their interaction with bare, as well as with gold cluster and Ti adatom covered, black phosphorene (α-P) monolayers using density functional theory. The binding of these amino acids and nucleobases to the bare α-P monolayer is realized generally through weak van der Waals interaction and comprises only a small amount of charge exchange. Accordingly, the electronic energy structures of adsorbates and underlying substrate are not affected significantly. However, the electronic structure of bare α-P is significantly affected upon adsorption of a gold cluster and a single Ti adatom; depending on the size of the adsorbate and the symmetry of their coverage, the energy band gap can be tuned and permanent magnetic moments can be attained. Additionally, the adsorption of amino acids or nucleobases to these adsorbates on an α-P monolayer results in enhanced binding and hence makes their sustainable fixation on α-P monolayer possible. In particular, a semiconducting Au decorated α-P monolayer undergoes a metal−insulator transition upon the adsorption of tyrosine. This and similar effects favor the α-P monolayer in biosensor applications. In contrast to the situation with adsorbates, the binding of amino acid is not enhanced when it adsorb to patterned vacancy or divacancy sites of the α-P monolayer. Our study shows that the absorbance of the bare α-P monolayer can be enhanced by coating with amino acid and nucleobases. The absorbance spectrum can be further modified by the adsorption of these molecules to gold atoms on the α-P monolayer.

show abstract

Polypyrrole-Au Nanoparticles Composite as Suitable Platform for DNA Biosensor with Electrochemical Impedance Spectroscopy Detection

Cited by 38 publications

References 40 publications

DC electrical conductivity and rate of ammonia vapour-sensing performance of synthetic polypyrrole–zirconium(IV) phosphate cation exchange nanocomposite

DC electrical conductivity and rate of ammonia vapour-sensing performance of synthetic polypyrrole–zirconium(IV) phosphate cation exchange nanocomposite

Progress in the Development of Intrinsically Conducting Polymer Composites as Biosensors

Enhanced Interactions of Amino Acids and Nucleic Acid Bases with Bare Black Phosphorene Monolayer Mediated by Coadsorbed Species

Contact Info

Product

Resources

About