Polysaccharide microarrays with a CMOS based signal detection unit

Baader, Johannes; Klapproth, Holger; Bednar, Sonja; Brandstëtter, Thomas; Rühe, Jürgen; Lehmann, Mirko; Freund, Ingo

doi:10.1016/j.bios.2010.01.021

Cited by 19 publications

(9 citation statements)

References 37 publications

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“…Our group [13], [33], [34], [35] and others [16], [17], [36], [37], [38], [39], [40], [41], [42], [43], [44], [45] have used glycan array technology to profile repertoires of serum antibodies in humans, but much less is known about macaque antibody repertoires and the optimal methods for measuring their levels on a glycan array. In addition, the macaque samples available for this study were heat treated to inactivate SIV and the set included a mixture of serum and plasma.…”

Section: Resultsmentioning

confidence: 99%

High-Throughput Profiling of Anti-Glycan Humoral Responses to SIV Vaccination and Challenge

et al. 2013

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Recent progress toward an HIV vaccine highlights both the potential of vaccines to end the AIDS pandemic and the need to boost efficacy by incorporating additional vaccine strategies. Although many aspects of the immune response can contribute to vaccine efficacy, the key factors have not been defined fully yet. A particular area that may yield new insights is anti-glycan immune responses, such as those against the glycan shield that HIV uses to evade the immune system. In this study, we used glycan microarray technology to evaluate anti-glycan antibody responses induced by SIV vaccination and infection in a non-human primate model of HIV infection. This comprehensive profiling of circulating anti-glycan antibodies found changes in anti-glycan antibody levels after both vaccination with the Ad5hr-SIV vaccine and SIV infection. Notably, SIV infection produced generalized declines in anti-glycan IgM antibodies in a number of animals. Additionally, some infected animals generated antibodies to the Tn antigen, which is a cryptic tumor-associated antigen exposed by premature termination of O-linked glycans; however, the Ad5hr-SIV vaccine did not induce anti-Tn IgG antibodies. Overall, this study demonstrates the potential contributions that glycan microarrays can make for HIV vaccine development.

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Section: Resultsmentioning

confidence: 99%

High-Throughput Profiling of Anti-Glycan Humoral Responses to SIV Vaccination and Challenge

et al. 2013

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“…As a result, the saturation of the photodiode is prevented without an optical filter and the distance from the tag to the surface of the photodetector is shortened [ 82 ]. Baader et al [ 34 ] developed polysaccharide microarrays and a CMOS-based electric signal readout process for the chemiluminescence-based detection of anti-polysaccharide IgG antibodies in human blood serum. In this system, unmodified pneumococcal polysaccharides from S. pneumonia were directly printed onto silicon photodiode surfaces (see Table 4 ).…”

Section: Cmos Sensors and Circuitsmentioning

confidence: 99%

“…Recent decades have witnessed unprecedented advances in CMOS sensors using optical [ 26 ], electrochemical [ 27 , 28 , 29 ], and magnetic [ 30 , 31 ] techniques alike for a variety of applications such as for the detection of different viruses (such as human respiratory viruses [ 32 , 33 ], Zika virus [ 27 ], and dengue virus [ 30 ]), as well as monitoring various bacteria (e.g., Streptococcus pneumoniae [ 34 ], bacillus globigii [ 35 ], Staphylococcus epidermidis [ 26 ], and Escherichia coli [ 28 ]) and detecting parasites (such as Plasmodium falciparum malaria diagnosis [ 29 ]). There are other opportunities for reconfiguring these devices for the diagnosis of similar diseases.…”

Section: Introductionmentioning

confidence: 99%

Towards Fully Integrated Portable Sensing Devices for COVID-19 and Future Global Hazards: Recent Advances, Challenges, and Prospects

2021

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Since the onset of the coronavirus disease 2019 (COVID-19) pandemic, this fatal disease has been the leading cause of the death of more than 3.9 million people around the world. This tragedy taught us that we should be well-prepared to control the spread of such infectious diseases and prevent future hazards. As a consequence, this pandemic has drawn the attention of many researchers to the development of portable platforms with short hands-on and turnaround time suitable for batch production in urgent pandemic situations such as that of COVID-19. Two main groups of diagnostic assays have been reported for the detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including nucleic acid-based and protein-based assays. The main focus of this paper is on the latter, which requires a shorter time duration, less skilled technicians, and faces lower contamination. Furthermore, this paper gives an overview of the complementary metal-oxide-semiconductor (CMOS) biosensors, which are potentially useful for implementing point-of-care (PoC) platforms based on such assays. CMOS technology, as a predominant technology for the fabrication of integrated circuits, is a promising candidate for the development of PoC devices by offering the advantages of reliability, accessibility, scalability, low power consumption, and distinct cost.

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“…To date, however, microbial glycan arrays have been created mainly from isolated carbohydrates that were derivatized for immobilization purposes (23). Depending on the experimental objective, a few (24)(25)(26)(27), dozens (28,29), about 150 (30,31), and even up to 300 (32) of such isolated and characterized carbohydrate preparations have been immobilized. Shotgun glycan arrays exposing the fractionated but uncharacterized glycome of a given sample have revealed important binding information about native receptors albeit with lower resolution and structural control than synthetic arrays would allow (33,34).…”

Section: Significancementioning

confidence: 99%

Microbe-focused glycan array screening platform

Geissner

Reinhardt

Rademacher

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein–glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.

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Polysaccharide microarrays with a CMOS based signal detection unit

Cited by 19 publications

References 37 publications

High-Throughput Profiling of Anti-Glycan Humoral Responses to SIV Vaccination and Challenge

High-Throughput Profiling of Anti-Glycan Humoral Responses to SIV Vaccination and Challenge

Towards Fully Integrated Portable Sensing Devices for COVID-19 and Future Global Hazards: Recent Advances, Challenges, and Prospects

Microbe-focused glycan array screening platform

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