Polysaccharide production by Aureobasidium pullulans: factors affecting polysaccharide formation

Badr-Eldin, Samir M.; El–Tayeb, O. M.; El-Masry, H. G.; Mohamad, Fihrina; El‐Rahman, Ola A. Abd

doi:10.1007/bf00144465

Cited by 39 publications

(12 citation statements)

References 4 publications

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“…In previous studies, A. pullulans strain NRRL 6220 gave a higher EPS yield (32.7 g l -1 ) on (NH 4 ) 2 SO 4 than on other nitrogen substrates including ammonium citrate, NaNO 3 , and urea [1]. On the other hand, A. pullulans strain ATCC 42023 has given greater pullulan yields on complex nitrogen sources including peptone than that on ammonium sulfate [17].…”

Section: Resultsmentioning

confidence: 99%

Pullulan production by tropical isolates of Aureobasidium pullulans

Prasongsuk

Berhow

Dunlap

et al. 2006

J Ind Microbiol Biotechnol

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Tropical isolates of Aureobasidium pullulans previously isolated from distinct habitats in Thailand were characterized for their capacities to produce the valuable polysaccharide, pullulan. A. pullulans strain NRM2, the so-called "color variant" strain, was the best producer, yielding 25.1 g pullulan l(-1) after 7 days in sucrose medium with peptone as the nitrogen source. Pullulan from strain NRM2 was less pigmented than those from the other strains and was remarkably pure after a simple ethanol precipitation. The molecular weight of pullulan from all cultures dramatically decreased after 3 days growth, as analyzed by high performance size exclusion chromatography. Alpha-amylase with apparent activity against pullulan was expressed constitutively in sucrose-grown cultures and induced in starch-grown cultures. When the alpha-amylase inhibitor acarbose was added to the culture medium, pullulan of slightly higher molecular weight was obtained from late cultures, supporting the notion that alpha-amylase plays a role in the reduction of the molecular weight of pullulan during the production phase.

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Section: Resultsmentioning

confidence: 99%

Pullulan production by tropical isolates of Aureobasidium pullulans

Prasongsuk

Berhow

Dunlap

et al. 2006

J Ind Microbiol Biotechnol

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“…It has been reported that the pullulan-producing strains of A. pullulans prefer ammonium as the sole nitrogen source for their EPS production, whereas that of the aubasidan-producing strains is nitrate 6,8,9 . Hence the effect of nitrogen source on the EPS production by NRRL 58539, NRRL 58543 and NRRL 58560 was investigated using Production Medium as previously described 3 .…”

Section: Methodsmentioning

confidence: 99%

An aubasidan-like β-glucan produced by Aureobasidium pullulans in Thailand

Unhapattaratitikul

Seelanan²,

Punnapayak³

2013

ScienceAsia

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ABSTRACT:The nutrient assimilation profile of two non-pullulan-producing strains of Aureobasidium pullulans (NRRL 58539 and NRRL 58543) isolated in Thailand was investigated. Similar nutrient assimilation was observed between both strains and the pullulan-producing A. pullulans NRRL 58560. All three strains weakly assimilated methyl-α-D-glucoside and lactose. For the exopolysaccharide (EPS) production, NRRL 58539 and NRRL 58543 preferred NaNO3 as the sole nitrogen source. Enzyme sensitivity assays, infrared spectroscopy, and nuclear magnetic resonance analyses of the EPSs revealed a broad structural similarity to aubasidan and not to pullulan. This is the first report of an aubasidan-like β-glucan produced by A. pullulans in Thailand.

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“…After removal of the residual ethanol, the precipitate was dissolved in 3 ml of deionized water at 80 o C and the solution was dialyzed against deionized water for 48h to remove small molecules in the solution. The exopolysaccharide was precipitated again by using 6 ml of the cold ethanol and the residual ethanol was removed, the precipitate was then dried at 80 o C to a constant weight (Badr-Eldin et al, 1994). Pullulan weight was measured using electronic balance (Sartorius Limited, U.S.A.) and expressed in gram/liter.…”

Section: Isolation and Purification Of Extracellular Polysaccharidementioning

confidence: 99%

A novel fermentor system optimized for continuous production of pullulan

Singh¹,

Gaur²,

Jamal³

et al. 2013

Afr. J. Biotechnol.

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A novel fermentor system containing Aureobasidium pullulans in polyurethane foam was designed to achieve continuous production of pullulan. A. pullulans cells were immobilized in 10 to 20 g polyurethane foam with pore size of 1000 Ǻ. The system has specialized aeration provision with 80 perforations of 4 mm. Pullulan production achieved was approximately 37g/l in 18 cycles at 42°C, pH 5.5 and at aeration rate of 0.5 vvm. The concentration of sucrose and ammonium sulphate was 3 and 0.5%, respectively. This novel design could serve as an excellent fermentor system in industries for large scale continuous production of pullulan.

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Polysaccharide production by Aureobasidium pullulans: factors affecting polysaccharide formation

Cited by 39 publications

References 4 publications

Pullulan production by tropical isolates of Aureobasidium pullulans

Pullulan production by tropical isolates of Aureobasidium pullulans

An aubasidan-like β-glucan produced by Aureobasidium pullulans in Thailand

A novel fermentor system optimized for continuous production of pullulan

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