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Aureobasidium pullulans NRRL 6220 synthesized polysaccharide most actively in media containing sucrose, fructose or maltose with (NH4)2SO4 (0.6 g/l) or ammonium acetate giving greatest yields of the polysaccharide. With (NH4)2SO4 at ≥1.2 g/l, production of polysaccharide was decreased considerably. Polysaccharide production was highest with an initial pH of 6.5 while biomass formation was better below an initial pH of 5.5. Optimum phosphate concentration for polysaccharide production was 0.03 M.

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Chemical analysis of corn steep liquor, produced from wet milling of corn grains, revealed the presence of 13% total soluble solids, 5.2% proteins and 2.5% ninhydrin reacting compounds. A cheap method, depending upon pH adjustment, was devised to obtain large amounts of precipitates with protein content up to 43 %. The supernatants resulting after separation of the precipitates were tested as a growth medium for production of fodder yeast Candida utilis. The supernatants supported good growth of the yeast without addition of extraneous sources of nutrients or vitamins. Depending on the supernatant yields ranging between 15 to 29 g of dry yeast per litre were obtained per litre with protein content 38 to 55 %. The industrial application of these findings is discussed.

No abstract