Polysaccharide production by plant cells in suspension: experiments and mathematical modeling

Glicklis, Rachel; Mills, David; Sitton, D.; Stortelder, W.J.H.; Merchuk, José C.

doi:10.1002/(sici)1097-0290(19980320)57:6<732::aid-bit10>3.0.co;2-9

Cited by 19 publications

(7 citation statements)

References 21 publications

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“…Simulated graphs for dry biomass and substrate concentration (left axis) and cell viability (right axis) each at 15°C respective 25°C, modeled with data of S. officinale batch cultures from Glicklis et al () simulated by the authors using Berkeley Madonna™.…”

Section: Models Concerning Plant Suspension Cultures (Su)mentioning

confidence: 99%

“…Three years later, Glicklis et al () published a structural model with focus on polysaccharide production, approved with experimental data of a Symphytum officinale (common comfrey) culture. These empirical data were taken from shake flask experiments at four different temperatures; an exemplary implementation of this demonstrative model with cell dry weight c X , substrate concentration c s , and viability V for 15 and 25°C is shown in Figure .…”

Section: Models Concerning Plant Suspension Cultures (Su)mentioning

confidence: 99%

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Modeling of plant in vitro cultures: Overview and estimation of biotechnological processes

Maschke

Geipel

Bley

2014

Biotech & Bioengineering

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Plant cell and tissue cultivations are of growing interest for the production of structurally complex and expensive plant-derived products, especially in pharmaceutical production. Problems with up-scaling, low yields, and high-priced process conditions result in an increased demand for models to provide comprehension, simulation, and optimization of production processes. In the last 25 years, many models have evolved in plant biotechnology; the majority of them are specialized models for a few selected products or nutritional conditions. In this article we review, delineate, and discuss the concepts and characteristics of the most commonly used models. Therefore, the authors focus on models for plant suspension and submerged hairy root cultures. The article includes a short overview of modeling and mathematics and integrated parameters, as well as the application scope for each model. The review is meant to help researchers better understand and utilize the numerous models published for plant cultures, and to select the most suitable model for their purposes.

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Section: Models Concerning Plant Suspension Cultures (Su)mentioning

confidence: 99%

Section: Models Concerning Plant Suspension Cultures (Su)mentioning

confidence: 99%

Modeling of plant in vitro cultures: Overview and estimation of biotechnological processes

Maschke

Geipel

Bley

2014

Biotech & Bioengineering

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“…These features, which adversely affect the cultivation results, arise from increased culture broth viscosity and stable foam layers. These are caused by secreted cell wall polysaccharides 50, 51 and proteins, and also by the microsparger 52, which is the aeration device in the S.U.B.…”

Section: Stirred Bag Bioreactorsmentioning

confidence: 99%

Disposable bioreactors for plant liquid cultures at Litre‐scale

Eibl

Werner

Eibl

2009

Engineering in Life Sciences

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Driven by the demands of the market and the manufacturing industry, disposable bioreactors have gained in importance in cell culture‐based processes during the last 10 years. Today they are widely accepted in R&D and also in manufacturing where process simplicity, safety and flexibility have top priority. Although disposable bioreactors are mainly used for cell expansions, glycoprotein secretions and virus generations realised with mammalian and insect cell lines, there are several reports delineating their suitability for the cultivation of plant cell and tissue cultures. This review describes the current disposable bioreactor types suitable for growing plant cell suspensions and organ cultures (hairy roots, meristematic clusters, somatic embryos) at Litre‐scale. Based on a definition of the term “disposable bioreactor”, a categorisation of the prevalent types for plant liquid cultures is presented. We describe the bioreactor regimes, working principles and bioengineering parameters of mechanically and pneumatically agitated bag bioreactors, which have advantages of process scalability and efficiency. Furthermore, results from the literature and data from our own research (obtained during production of undifferentiated bioactive cells, expressions of secondary metabolites and glycoproteins, and micropropagations of plant tissues) are discussed.

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“…4). Although lysis of the more resistant viable cells (Glicklis et al, 1998) is likely to occur at a lower specific rate than for the more fragile TB dead cells, for simplicity, a single rate was assumed for all cell lysis. Thus, if we assume that viable and TB dead cells lyse at an identical specific rate k l , and viable cells are transformed into TB dead cells with a specific rate k b , the viable cell balance can be written:…”

Section: Mechanism Of Cell Death and Lysismentioning

confidence: 99%

Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release

Steward

Martin

Engasser

et al. 1999

Biotechnol. Bioeng.

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The death of Medicago sativa L. cells cultivated in a batch culture was investigated by measuring both the appearance of intact dead cells determined on the basis of the trypan blue (TB) dye exclusion, and the release of the cytoplasmic esterase activity into the culture medium upon cell death. Taking into account the strong instability of this released esterase activity, the total dead cell and lysed cell densities have been estimated. A mechanism for cell death and lysis is proposed and the specific rates of cell growth, death and lysis estimated. The specific rate of appearance of TB dead cells was low and essentially constant (0.25 day−1) during the first 8 days of the batch culture, and then increased above 1.5 day−1 after 2 weeks of cultivation. Whereas no lysis occurred during the first seven days, this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process. Thus, the viability determined by the trypan blue exclusion method appeared to be invalid after 7 days of culture. When lysis of viable cells is taken into consideration, the specific growth rate was significantly increased and growth was shown to continue for a further 8 days. Increased sensitivity of the cells to shear stresses and consequent cell lysis could be the result of a 35% increase in the cell size © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 66: 114–121, 1999.

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Polysaccharide production by plant cells in suspension: experiments and mathematical modeling

Cited by 19 publications

References 21 publications

Modeling of plant in vitro cultures: Overview and estimation of biotechnological processes

Modeling of plant in vitro cultures: Overview and estimation of biotechnological processes

Disposable bioreactors for plant liquid cultures at Litre‐scale

Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release

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