Polysaccharides from Dendrobium officinale inhibit proliferation of osteosarcoma cells and enhance cisplatin-induced apoptosis

Zhang, Xiaofeng; Duan, Shuna; Tao, Shengchang; Huang, Jian; Liu, Chenxing; Xing, Shangping; Ren, Zhiyao; Lei, Zhouxi; Li, Yunrong; Wei, Gang

doi:10.1016/j.jff.2020.104143

Cited by 23 publications

(10 citation statements)

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“…Three polysaccharide fractions, DOP2 (D2), DOP8 (D8), and DOP122 (D122), with a Mw (in kDa) of 24.89, 80.32, and 1,224.54, respectively, were obtained from the stems of D . officinale , as described previously ( Zhang et al, 2020 ). In brief, after hot water extraction, a D .…”

Section: Methodsmentioning

confidence: 99%

“…D . officinale polysaccharides possess anticancer ( Yu et al, 2018a ; Zhang et al, 2019 ; Zhang et al, 2020 ; Tao et al, 2021 ), immunomodulation ( Huang et al, 2016 ; Tao et al, 2019 ), antioxidant ( Luo et al, 2016 ), hypoglycemic ( Wang et al, 2018 ; Kuang et al, 2020 ) and antifatigue ( Wei et al, 2017 ) activities. However, few studies have focused on the activity of D .…”

Section: Introductionmentioning

confidence: 99%

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Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

et al. 2021

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We investigated the antitumor effects of four fractions of Dendrobium officinale Kimura & Migo (D. officinale) polysaccharides with different molecular weights (Mw), Astragalus membranaceus polysaccharides (APS) and Lentinus edodes polysaccharides (LNT) on colorectal cancer (CRC) using a zebrafish xenograft model. Transcriptome sequencing was performed to further explore the possible antitumor mechanisms of D. officinale polysaccharides. Fractions of D. officinale polysaccharides, LNT, and APS could significantly inhibit the growth of HT-29 cells in a zebrafish xenograft model. One fraction of D. officinale polysaccharides called DOPW-1 (Mw of 389.98 kDa) exhibited the strongest tumor inhibition. Compared with the control group, RNA-seq revealed that the DOPW-1–treated experimental group had 119 differentially expressed genes (DEGs), of which 45 had upregulated expression and 74 had downregulated expression. Analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes suggested that the pathway “apoptosis-multiple species” was the most significantly enriched. Our data indicated that 1) fractions of D. officinale polysaccharides of Mw 389.98 kDa were most suitable against CRC; 2) DOPW-1 could be developed into a clinical agent against CRC; and 3) an apoptosis pathway is important for DOPW-1 to inhibit the proliferation of HT-29 cells.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

et al. 2021

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“…On the contrary, if the relative molecular weight is too low, polysaccharides cannot form active-polymer structures such as triple-helical conformation ( 10 ). For example, the polysaccharide with moderate molecular weight from Dendrobium officinale showed the strongest inhibitory effect on the cells ( 11 ). Polysaccharides with less than 5 kDa or more than 400 kDa showed marginal immunomodulatory activity or lost the activity directly ( 12 ).…”

Section: Structure-activity Relationshipmentioning

confidence: 99%

Structural Elucidation, Modification, and Structure-Activity Relationship of Polysaccharides in Chinese Herbs: A Review

et al. 2022

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Chinese herbal polysaccharides (CHPs) are natural polymers composed of monosaccharides, which are widely found in Chinese herbs and work as one of the important active ingredients. Its biological activity is attributed to its complex chemical structure with diverse spatial conformations. However, the structural elucidation is the foundation but a bottleneck problem because the majority of CHPs are heteropolysaccharides with more complex structures. Similarly, the studies on the relationship between structure and function of CHPs are even more scarce. Therefore, this review summarizes the structure-activity relationship of CHPs. Meanwhile, we reviewed the structural elucidation strategies and some new progress especially in the advanced structural analysis methods. The characteristics and applicable scopes of various methods are compared to provide reference for selecting the most efficient method and developing new hyphenated techniques. Additionally, the principle structural modification methods of CHPs and their effects on activity are summarized. The shortcomings, potential breakthroughs, and developing directions of the study of CHPs are discussed. We hope to provide a reference for further research and promote the application of CHPs.

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“…Pharmacopoeia of the People’s Republic of China contains Dendrobium and Dendrobium officinale , Dendrobium including Dendrobium nobile Lindl., Dendrobium huoshanense C. Z. Tang et S. J. Cheng, Dendrobium chrysotoxum Lindl., and Dendrobium fimbriatum Hook. Dendrobium officinale Kimura et Migo, as a rare traditional Chinese medicine, has been listed separately and distinguished from other Dendrobium since the 2010 edition of Ch.P because of its great pharmacological value (Ng et al, 2012; Tao et al, 2019; Zhang et al, 2020), and mainly distributed in Yunnan, Sichuan, Guizhou, Guangxi and Zhejiang provinces of China (Shiau et al, 2005). Due to its harsh growth conditions, slow growth rate and low natural germination rate of seeds, D. officinale is difficult to grow, and excessive mining also has led to a serious shortage of natural resources and high price (Ding et al, 2002; Yang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Taxonomic and functional diversity of Dendrobium officinale microbiome in Danxia habitat

Wang

Liang

et al. 2022

Journal of Applied Microbiology

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Aims Microbial communities that inhabit plants are crucial for plant survival and well‐being including growth in stressful environments. The medicinal plant, Dendrobium officinale grows in the barren soils of the Danxia Habitat. However, the microbiome composition and functional potential for growth of this plant in this environment are still unexplored. Methods and Results In this study, we analysed the taxonomic and functional diversity of the D. officinale Microbiome by metagenomic sequencing of both rhizosphere and endosphere samples. A total of 155 phyla, 122 classes, 271 orders, 620 families and 2194 genera were identified from all samples. The rhizospheric microbes (DXRh) were mainly composed of Proteobacteria and Acidobacteria, while Basidiomycota and Ascomycota were the most dominant phyla in root endosphere (DXRo) and stem endosphere (DXS), respectively. Most of the dominant microbial communities had been reported to have diverse functional potentials that can help plant growth and development in stressful and nutrient‐deprived ecological environmental. These include plant growth promoting rhizobacteria (PGPR) such as Massilia, Pseudomonas, Bradyrhizobium, Klebsiella, Streptomyces, Leclercia, Paenibacillus, Frankia and Enterobacter in the DXRh, Tulasnella and Serendipita in the DXRo, Colletotrichum and Burkholderia in the DXS and Paraburkholderia, Rhizophagus and Acetobacter in endosphere. Analysis using the KEGG, eggNOG and CAZy databases showed that metabolic pathways such as carbohydrate metabolism, amino acid metabolism, energy metabolism, genetic information processing and environmental information processing are significantly abundant, which may be related to the survival, growth and development of D. officinale in a stressful environment. Conclusions We speculated that the microbial community with diverse taxonomic structures and metabolic functions inhabiting in different niches of plants supports the survival and growth of D. officinale in the stressful environment of Danxia Habitat. Significance and Impact of the Study This study provided an important data resource for microbes associated with D. officinale and theoretical foundation for further studies.

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Polysaccharides from Dendrobium officinale inhibit proliferation of osteosarcoma cells and enhance cisplatin-induced apoptosis

Cited by 23 publications

References 50 publications

Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

Structural Elucidation, Modification, and Structure-Activity Relationship of Polysaccharides in Chinese Herbs: A Review

Taxonomic and functional diversity of Dendrobium officinale microbiome in Danxia habitat

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