Polysaccharides in fungi. XXVI. Two branched (1.RAR.3)-.BETA.-D-glucans from hot water extract of Yu er.

Abstract

Two water-insoluble glucans, U-3-N ([alpha]D +1.0 degree, 0.5 M sodium hydroxide) and U-3-AP1 ([alpha]D +2.5 degrees, 1 M sodium hydroxide) were isolated from hot-water extract of the fruiting bodies of Yũ ĕr (Chinese name) (Auricularia sp.). U-3-N and U-3-AP1 were investigated by a combination of chemical and spectroscopic methods. The results indicated that U-3-N (molecular weight, 6.1 x 10(5)) was similar to beta-(1----6)-branched (1----3)-beta-D-glucan (N-5P: molecular weight, 5.6 x 10(5)) isolated from the alkaline extract of the fruiting bodies, and U-3-AP1 (molecular weight, 6.3 x 10(4)) was beta-(1----6)-branched (1----3)-beta-D-glucan containing beta-(1----6)-linked D-glucopyranosyl residues. U-3-N showed potent anti-tumor activity against the solid form of sarcoma 180, although U-3-AP1 had little effect on the tumor.

Chemical modification and antitumor activities of two polysaccharide–protein complexes from Pleurotus tuber-regium

“…1. The TM1 and TM2 samples exhibited absorption peak at 890 cm −1 , which was characteristic of ␤-d-glucan [24]. The IR absorption at 800 cm −1 for TM1 was the characteristic absorption of mannose.…”

β-Glucan Synthase Gene Overexpression and β-Glucans Overproduction in Pleurotus ostreatus Using Promoter Swapping

“…7. The absorption peaks at 3,457 cm −1 were ascribed to hydroxyl stretching vibration of the polysaccharide; peaks at 3,151 cm −1 were CH (CH3, CH2, or CH) stretching vibration; those at 1,626 cm −1 attributed to C = O asymmetric stretching vibration [24]; those at 1,398 cm −1 were due to C-H variable-angle vibration; peaks at 1,143 to 1,088 cm −1 were characteristics of C-O-C asymmetric vibration of pyranose ring [25]; finally the bands at 856 cm −1 mapped to β-glucan [26], suggesting that β-glycosidic bond was present in all samples.…”

Determination of molecular size and shape of hyperbranched polysaccharide in solution

“…24 The proportion of terminal glucose in TM3b is much higher than the others, suggesting a hyperbranched polysaccharide. 25 The degree of branching (DB) is one of the important molecular parameters of the hyperbranched polymer, which is related to many physical properties.…”