Polysaccharides of mushroom Pleurotus spp.: New extraction techniques, biological activities and development of new technologies

Barbosa, Jhonatas Rodrigues; Freitas, Maurício Madson dos Santos; Martins, Luiza Helena da Silva; Carvalho, Rubens Côrte Real de

doi:10.1016/j.carbpol.2019.115550

Cited by 71 publications

(31 citation statements)

References 223 publications

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“…isolates examined by polyacrylamide gel electrophoresis isoelectric and protein blotting, however, demonstrated that P. columbinus (Figure 1) could be regarded as a separate taxon. Despite there being many studies about the phytochemical composition of Pleurotus spp., also highlighted by recent reviews [33][34][35] there are very few studies dealing with the phytochemistry, antioxidant and antimicrobial activities of P. columbinus Quél [36,37]. Metabolomics is a new discipline which is defined as the monitoring of metabolite concentration in fungi, bacteria and plants.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Profiling and Biological Activities of Pleurotus columbinus Quél. Cultivated on Different Agri-Food Byproducts

et al. 2021

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The genus Pleurotus (Fr.) P. Kumm (Pleurotaceae, Basidiomycota) comprises a cosmopolitan group of mushrooms highly appreciated for their nutritional value and health-promoting benefits. Despite there being many studies about the phytochemical composition of Pleurotus spp., there are very few reports dealing with the phytochemistry, antioxidant and antimicrobial activities of P. columbinus Quél. In this study, a mass spectrometry ultra-performance liquid chromatography mass spectrometry (UHPLC)-QTOF method, coupled with principal component analysis (PCA), was applied to the P. columbinus metabolome in order to investigate the influence of different agri-food residues as growth substrates for P. columbinus cultivation, on the bioactive chemical profile of fruiting bodies and evaluated their potential as antioxidants and antimicrobials. Additionally, a quantitative HPLC-DAD-MS analysis was conducted on phenolic and flavonoid compounds, that could explain, albeit partially, the observed biological effects of P. columbinus extracts. The qualitative metabolic profile identified 97 metabolites, whereas the quantitative HPLC-DAD-MS analysis confirmed the presence of phenolic and flavonoids, in the mushroom extracts, which also showed intrinsic scavenging/reducing and antimicrobial effects. The antibacterial effects were particularly evident against Escherichia coli, whereas Tricophyton and Aspergillus were the dermatophytes more sensitive to the mushroom extracts. The present study supports more in-depth investigations, aimed at evaluating the influence of growth substrate on P. columbinus antimicrobial and antioxidant properties. The extracts from P. columbinus revealed valuable sources of primary and secondary metabolites, thus suggesting potential applications in the formulation of food supplements with biological properties, above all in terms of antioxidant and antimicrobial properties.

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Section: Introductionmentioning

confidence: 99%

Metabolomic Profiling and Biological Activities of Pleurotus columbinus Quél. Cultivated on Different Agri-Food Byproducts

et al. 2021

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“…Polysaccharide is defined as a natural macromolecule compound consisting of 10 or more monosaccharides linked by glycosidic bonds and commonly found in plants, animals and microorganisms [1] . A growing number of researches have shown that edible fungus polysaccharides are often used as nutrients to regulate immune activity and have massive biological activities, such as antitumor, antioxidant, anti‐inflammatory, hypoglycemic, and hepatoprotective effects [1–4] . And researchers also found that the biological activities of polysaccharides are closely linked to its chemical structures [5–7] …”

Section: Introductionmentioning

confidence: 99%

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Niu

Liu

et al. 2021

Chemistry & Biodiversity

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In the present study, a novel cold water‐soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated. The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853. Its backbone was composed of α‐D‐Glu, α‐D‐Xyl, α‐D‐Gal and α‐L‐Fuc. The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose‐dependent manner. By analyzing Annexin V‐FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria‐mediated apoptotic pathway. Our results could provide valuable information for the potential application of LGP as an anti‐hepatoma agent.

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“…Recently, Barbosa and de Carvalho Junior stated that the immunomodulatory potential of polysaccharides is used to assess the ability of these biopolymers to enhance the body immunity towards viral infections, especially SARS-CoV-2 [13]. Polysaccharides from various natural sources such as plants [14], algae [15], fungi [16][17][18] stimulates the innate and cellular community via interactions with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes [19]. When stimulated, the macrophage can destroy the pathogens directly by phagocytosis and indirectly through the secretions of NO and various cytokines TNF-α, IL-1β, and IL-6 [20].…”

Section: Introductionmentioning

confidence: 99%

Structural Elucidation and Immunostimulatory Activities of Quinoa Non-starch Polysaccharide Before and After Deproteinization

Cao

Palanisamy

et al. 2021

J Polym Environ

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Non-starch polysaccharides derived from natural resources play a significant role in the field of food science and human health due to their extensive distribution in nature and less toxicity. In this order, the immunostimulatory activity of a non-starch polysaccharide (CQNP) from Chenopodium quinoa was examined before and after deproteination in murine macrophage RAW 264.7 cells. The chemical composition of CQNP and deproteinated-CQNP (D-CQNP) were spectrometrically analysed that revealed the presence of carbohydrate (22.7 ± 0.8% and 39.5 ± 0.8%), protein (41.4 ± 0.5% and 20.8 ± 0.5%) and uronic acid (8.7 ± 0.3% and 6.7 ± 0.2%). The monosaccharide composition results exposed that CQNP possesses a high amount of arabinose (34.5 ± 0.3) followed by galactose (26.5 ± 0.2), glucose (21.9 ± 0.3), rhamnose (7.0 ± 0.1), mannose (6.0 ± 0.1) and xylose (4.2 ± 0.2). However, after deproteination, a difference was found in the order of the monosaccharide components, with galactose (41.1 ± 0.5) as a major unit followed by arabinose (34.7 ± 0.5), rhamnose (10.9 ± 0.2), glucose (6.6 ± 0.2), mannose (3.4 ± 0.2) and xylose (3.2 ± 0.2). Further, D-CQNP potentially stimulate the RAW 264.7 cells through the production of nitric oxide (NO), upregulating inducible nitric oxide synthase (iNOS) and various pro-inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor-alpha (TNF-α). Moreover, stimulation of RAW 264.7 cells by D-CQNP takes place along the NF-κB and the MAPKs signaling pathways through the expression of cluster of differentiation 40 (CD40). This results demonstrate that RAW 264.7 cells are effectively stimulated after removal of the protein content in C. quinoa non-starch polysaccharides, which could be useful for develop a new immunostimulant agent.

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Polysaccharides of mushroom Pleurotus spp.: New extraction techniques, biological activities and development of new technologies

Cited by 71 publications

References 223 publications

Metabolomic Profiling and Biological Activities of Pleurotus columbinus Quél. Cultivated on Different Agri-Food Byproducts

Metabolomic Profiling and Biological Activities of Pleurotus columbinus Quél. Cultivated on Different Agri-Food Byproducts

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Structural Elucidation and Immunostimulatory Activities of Quinoa Non-starch Polysaccharide Before and After Deproteinization

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