Polysiloxane-based ratiometric fluorescent probe for monitoring lysosomal autophagy pathway in three distinguishable channels

Zhang, Kun; Ding, Guowei; Gai, Fengqing; Zhang, Yafang; Wang, Xiaoni; Gou, Zhiming; Zuo, Yujing

doi:10.1016/j.snb.2021.131023

Cited by 8 publications

(4 citation statements)

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“…Autophagy is an essential degradation process of cytoplasmic dysfunctional proteins and organelles in eukaryotic evolution [33] . Normal autophagy is connected with many important cellular physiological activities, such as regulating cell survival and death [34] . However, the abnormal autophagy of cells causes many diseases, including cancer, neurological disorders, and aging [35] .…”

Section: Resultsmentioning

confidence: 99%

“…[33] Normal autophagy is connected with many important cellular physiological activities, such as regulating cell survival and death. [34] However, the abnormal autophagy of cells causes many diseases, including cancer, neurological disorders, and aging. [35] Hence, monitoring the autophagy process is significant for bioscience and medicine.…”

Section: Monitoring Lysosomal Viscosity Changes During Autophagymentioning

confidence: 99%

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Computational chemistry‐assisted design of hydrazine‐based fluorescent molecular rotor for viscosity sensors

Dong,

Wang,

Yang

et al. 2023

Smart Molecules

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Deep understanding of the fluorescence quenching mechanisms of probes plays a crucial role in developing their practical applications. The fluorescence quenching mechanism of hydrazine‐based fluorescence probes needs to be clarified up to the present. Herein, we designed and synthesized a new hydrazine‐based fluorescence probe (HA‐Na) based on the naphthalimide skeleton. We clarified the molecular origin of the non‐fluorescence of this probe with the aid of computational chemistry and spectroscopic analysis. We showed that the significant rotation of the hydrazine group in the excited state potential energy surface, which caused the complete charge separation, was responsible for the fluorescence quenching of the probe in an organic solvent. Once the rotation was prevented in an aggregative state or high‐viscosity solution, the fluorescence of the probe recovered. In other words, the fluorescence quenching mechanism of hydrazine‐based fluorescence probes was attributed to the formation of a twisted intramolecular charge transfer (TICT) state. More importantly, we demonstrated that this fluorescence molecular rotor could be used to monitor the autophagy process in living cells by detecting lysosomal viscosity changes during starvation. Altogether, this work provides an essential theoretical basis for the developing potential hydrazine‐based fluorescence molecular rotors.

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Section: Resultsmentioning

confidence: 99%

Section: Monitoring Lysosomal Viscosity Changes During Autophagymentioning

confidence: 99%

Computational chemistry‐assisted design of hydrazine‐based fluorescent molecular rotor for viscosity sensors

Dong,

Wang,

Yang

et al. 2023

Smart Molecules

View full text Add to dashboard Cite

show abstract

“…8 and 10b). 143 It showed strong H + capture abilities for quantitatively imaging lysosomal pH during starvation-induced autophagy. This probe also detected changes in the intracellular pH upon treatment with the autophagy inhibitor chloroquine.…”

Section: Ratiometric Fluorescent Probes For Ph Mapping In Organellesmentioning

confidence: 99%

“…8 and 10b). 143 detection of lysosomal ΔpHi during autophagy. 145 Here, both the imidazole and dimethylamino groups could target lysosomes, and the benzimidazole group acted as a proton receptor for ratiometric fluorescence emission with a pK a of 4.73.…”

Section: Lysosome-targeting Ratiometric Fluorescent Probesmentioning

confidence: 99%