Mixed matrix membranes are considered as a novel generation of membranes that have favorable separation and stability properties of inorganic and polymeric membranes at the same time. In this study, the effects of presence of clay nanoparticles (CNs) on the enhancement of properties and performance of PVC nanofiltration membranes were investigated. FTIR, AFM, XRD, EDS, and FESEM analyses were used to evaluate the mixed matrix membranes. Significant improvements in the membrane hydrophilicity, porosity, permeation flux, and rejection were observed by incorporating CNs. The highest rejection of 94.8% was obtained with mixed matrix membranes containing 2 wt% of CNs. The permeation flux of this membrane was enhanced by 75% relative to the neat membrane.