Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants

Koryakov, Dmitry E.; Mal'ceva, N. I.; King, Robert C.; Жимулев, И. Ф.

doi:10.1385/1-59259-665-7:139

Cited by 5 publications

(6 citation statements)

References 25 publications

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“…First, the genome-wide distribution of Su(Hw), Mod67.2 and CP190 was examined using the giant NC chromosomes in the ovarian tumor ( otu 11 ) mutant (Koryakov et al, 2004). Incubation of otu 11 ovaries with antibodies against these three proteins showed extensive colocalization on euchromatic chromosome arms (Supplemental Figure 4).…”

Section: Resultsmentioning

confidence: 99%

“…The Cp190 mutants studied were 1) the Cp190 H4-1 allele that carries a premature stop codon that results in a carboxyl-terminal deletion of the last 331 amino acids (Pai et al, 2004), 2) Cp190 P11 that carries a deletion spanning from the 5’ end of Set to the 3’ end of MRG15 that removes Cp190 and four other genes (17), and 3) the Cp190 1 null allele that carries a premature stop codon that results in a carboxyl-terminal deletion of the last 964 amino acids (51). Polytene chromosome squashes from ovaries were completed using females carrying the ovarian tumor ( otu ) mutation ( y w sn 3 otu 11 ) (Koryakov et al, 2004). …”

Section: Methodsmentioning

confidence: 99%

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The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis

Baxley

Soshnev

Koryakov

et al. 2011

Developmental Biology

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129

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The Drosophila Suppressor of Hairy wing [Su(Hw)] insulator protein has an essential role in the development of the female germline. Here we investigate the function of Su(Hw) in the ovary. We show that Su(Hw) is universally expressed in somatic cells, while germ cell expression is dynamic. Robust levels accumulate in post-mitotic germ cells, where Su(Hw) localization is limited to chromosomes within nurse cells, the specialized cells that support oocyte growth. Although loss of Su(Hw) causes global defects in nurse cell chromosome structure, we demonstrate that these architectural changes are not responsible for the block in oogenesis. Connections between the fertility and insulator functions of Su(Hw) were investigated through studies of the two gypsy insulator proteins, Modifier of (mdg4)67.2 (Mod67.2) and Centrosomal Protein of 190 kD (CP190). Accumulation of these proteins is distinct from Su(Hw), with Mod67.2 and CP190 showing uniform expression in all cells during early stages of oogenesis that diminishes in later stages. Although Mod67.2 and CP190 extensively co-localize with Su(Hw) on nurse cell chromosomes, neither protein is required for nurse cell chromosome development or oocyte production. These data indicate that while the gypsy insulator function requires both Mod67.2 and CP190, these proteins are not essential for oogenesis. These studies represent the first molecular investigations of Su(Hw) function in the germline, which uncover distinct requirements for Su(Hw) insulator and ovary functions.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis

Baxley

Soshnev

Koryakov

et al. 2011

Developmental Biology

Self Cite

129

View full text Add to dashboard Cite

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“…Ovaries were isolated in Ephrussi-Beadle solution (129 mM NaCl, 4.7 mM KCl, and 1.9 mM CaCl 2 ) or in 0.7% NaCl, stained with acetic orcein, and squashed in 55% lactic acid. All procedures concerning squashes of NC polytene chromosomes are described in detail in Koryakov et al (2004).…”

Section: Polytene Chromosomesmentioning

confidence: 99%

“…Procedures for antibodies against HP1 (mouse monoclonal antibodies C1A9, dilution 1:50, obtained by S. Elgin) are described in detail in Koryakov et al (2004). Ovaries were isolated in Cohen and Gotchell Medium G with 0.5% NP-40 and fixed in 100 mM NaCl, 2 mM KCl, 20 mM NaH 2 PO 4 , 2% NP-40, and 2% formaldehyde.…”

Section: Antibodies and Immunostainingmentioning

confidence: 99%

The SuUR gene influences the distribution of heterochromatic proteins HP1 and SU(VAR)3–9 on nurse cell polytene chromosomes of Drosophila melanogaster

et al. 2006

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We have investigated the distribution of three heterochromatic proteins [SUppressor of UnderReplication (SUUR), heterochromatin protein 1 (HP1), and SU(VAR)3-9] in chromosomes of nurse cells (NCs) and have compared the data obtained with the distribution of the same proteins in salivary gland (SG) chromosomes. In NC chromosomes, the SU(VAR)3-9 protein was found in pericentric heterochromatin and at 223 sites on euchromatic arms, while in SG chromosomes, it was mainly restricted to the chromocenter. In NC chromosomes, the HP1 and SUUR proteins bind to 331 and 256 sites, respectively, which are almost twice the number of sites in SG chromosomes. The distribution of the HP1 and SU(VAR)3-9 proteins depends on the SuUR gene. A mutation in this gene results in a dramatic decrease in the amount of SU(VAR)3-9 binding sites in autosomes. In the X chromosome, these sites are relocated in comparison to the SuUR (+), and their total number only varies slightly. HP1 binding sites are redistributed in chromosomes of SuUR mutants, and their overall number did not change as considerably as SU(VAR)3-9. These data together point to an interaction of these three proteins in Drosophila NC chromosomes.

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“…A multitude of genes involved in different processes that affect NCCD, including factors such as pUf68, Squid, and Hrb27C, were also previously implicated in splicing in Drosophila [17], [18]. However, in the Drosophila germ line, the effects of these proteins on splicing were limited to studies of expression of Ovarian Tumor (Otu), a protein with obscure function that is required for female gamete development and germ-line chromosome organization [19], [20]; unexpectedly, the hnRNP Squid does not affect the alternative splicing of otu , but is required for expression of Otu protein. In addition, the hnRNP protein Hrb27C does not affect alternative splicing of the germ-line gene oskar , but is required for translational regulation [21].…”

Section: Introductionmentioning

confidence: 99%

Prp22 and Spliceosome Components Regulate Chromatin Dynamics in Germ-Line Polyploid Cells

et al. 2013

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During Drosophila oogenesis, the endopolyploid nuclei of germ-line nurse cells undergo a dramatic shift in morphology as oogenesis progresses; the easily-visible chromosomes are initially polytenic during the early stages of oogenesis before they transiently condense into a distinct ‘5-blob’ configuration, with subsequent dispersal into a diffuse state. Mutations in many genes, with diverse cellular functions, can affect the ability of nurse cells to fully decondense their chromatin, resulting in a ‘5-blob arrest’ phenotype that is maintained throughout the later stages of oogenesis. However, the mechanisms and significance of nurse-cell (NC) chromatin dispersal remain poorly understood. Here, we report that a screen for modifiers of the 5-blob phenotype in the germ line isolated the spliceosomal gene peanuts, the Drosophila Prp22. We demonstrate that reduction of spliceosomal activity through loss of peanuts promotes decondensation defects in NC nuclei during mid-oogenesis. We also show that the Prp38 spliceosomal protein accumulates in the nucleoplasm of nurse cells with impaired peanuts function, suggesting that spliceosomal recycling is impaired. Finally, we reveal that loss of additional spliceosomal proteins impairs the full decondensation of NC chromatin during later stages of oogenesis, suggesting that individual spliceosomal subcomplexes modulate expression of the distinct subset of genes that are required for correct morphology in endopolyploid nurse cells.

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Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants

Cited by 5 publications

References 25 publications

The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis

The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis

The SuUR gene influences the distribution of heterochromatic proteins HP1 and SU(VAR)3–9 on nurse cell polytene chromosomes of Drosophila melanogaster

Prp22 and Spliceosome Components Regulate Chromatin Dynamics in Germ-Line Polyploid Cells

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