Polyvinylferrocenium modified Pt electrode for the design of amperometric choline and acetylcholine enzyme electrodes

Şen, Songül; Gülce, Ahmet; Gülce, Handan

doi:10.1016/j.bios.2003.11.011

Cited by 47 publications

(8 citation statements)

References 29 publications

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“…This is the enzyme responsible for the synthesis of acetylcholine. Determination of ACh in the brain is important for understanding the mechanisms of neurotransmission and neuroregulation, which in turn should facilitate early detection and effective treatment of neurodegradative diseases, such as Alzheimer's disease [1][2][3][4][5][6][7][8]. Several methods for quantitative determination of ACh have recently been developed, including radiolabeling [9] and gas chromatographic techniques [10].…”

Section: Introductionmentioning

confidence: 99%

A novel matrix for the immobilization of acetylcholinesterase

Şahin

Demirel

Tümtürk

2005

International Journal of Biological Macromolecules

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Section: Introductionmentioning

confidence: 99%

A novel matrix for the immobilization of acetylcholinesterase

Şahin

Demirel

Tümtürk

2005

International Journal of Biological Macromolecules

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“…Working conditions for biosensors should be practical, so studies were performed at room temperature. In the literature, there are studies of ACh biosensor with optimum temperature value different from 60 °C, for example, 35 °C [18] and 55 °C [4]. These different values may be due to the type of polymer and immobilization used in the process.…”

Section: Resultsmentioning

confidence: 99%

“…1.1.3.17) with oxygen and water. By combining these two enzymes with the biosensor system, biosensors are being developed for use in the diagnosis of Alzheimer's disease [4‐6].…”

Section: Introductionmentioning

confidence: 99%

“…ACh could be detected by using various analytical methods like HPLC, GC‐MS, chemiluminescence, etc., but these methods are expensive, time‐consuming, and require a specialist to comment on the results. Biosensors are a kind of instrument that enables cheaper and rapid detection of analyte with low detection limit [4‐6]. This situation makes biosensors preferable sensors.…”

Section: Introductionmentioning

confidence: 99%

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A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot‐modified carbon paste electrode

Bodur

Dinç

Özmen

et al. 2020

Biotech and App Biochem

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Acetylcholine is a neurotransmitter, which is located at the intersections of the nerve and muscles in the lymph nodes of the internal organs motor systems and in various parts of the central nervous system. A decrease of acetylcholine in brain is associated with Alzheimer‘s disease. That is why it is an important agent for this disease. In this study, a bienzymatic biosensor system with acetylcholine esterase and choline oxidase was prepared with carbon paste electrode modified with carbon nano Dot‐(3‐Aminopropyl) triethoxysilane (CDs‐APTES) for determination of the amount of acetylcholine. Acetylcholine esterase and choline oxidase enzymes were immobilized onto a modified carbon paste electrode by cross‐linking with glutaraldehyde. Determination of acetylcholine was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the acetylcholine response of the prepared biosensor was investigated. In addition, the optimum CDs‐APTES amount, the linear operating range of the biosensor, and the interference effect were also investigated.

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“…Also, It has been reported that PVF + ClO 4 − matrix is selective to the anions and some anions can be inserted into the polymer through an anion-exchange process [30]. Using an anion-exchange process, a mild method developed binding of enzymes on PVF + ClO 4 − modified electrodes has been successfully applied to the design of the enzyme electrodes for glucose [31], sucrose [32], galactose [33], choline, and acetyl choline [34].…”

Section: Introductionmentioning

confidence: 98%