Pomegranate Micropropagation - A Review

Kumar, Ravi

doi:10.18782/2320-7051.5124

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…One of the antioxidant's role is preventing the oxidation of molecules. Free radicals are produced in oxidation reactions and can cause cell damage or death (Kumar et al, 2017). The addition of antioxidant in the medium at establishment stage is the best treatment for browning control (Hassan and Zayed, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The tissue culture has many benefits over the old propagation techniques, including uniform quality, seasonal independence, and rapid mass production of disease-free material and in vitro culture is the only mass propagation technique of healthy plants over a short period of time (Chauhan and Kanwar, 2012;Pal et al, 2014). In vitro propagation has been attempted in different pomegranate growing countries to propagate similar type and virus-free plants and there are some reports on tissue culture of pomegranate over the last few years (Kalalbandi et al, 2014;Kumar et al, 2017;Patil et al, 2011) and different samples such as shoot tips (Kalalbandi et al, 2014), nodal explants (Murkute et al, 2004), and cotyledons (Kanwar et al, 2010) were used. However, a more favorable method is the using of the axillary buds that is obtained from the nodal segments, as it ensures true to type of plants (Kalalbandi et al, 2014;Patil et al, 2011;Usharani et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Arid Zone Fruit Tree, Pomegranate, cvs. ‘Malase Yazdi’ and ‘Shirine Shahvar’

Mulaei¹,

Jafari²,

Shirmardi³

et al. 2019

International Journal of Fruit Science

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An optimum culture medium for micropropagation of pomegranate, cvs. 'Malase Yazdi' and 'Shirine Shahvar' is defined and protocols have been developed for in vitro shoot proliferation and rooting of the cultivars. Axillary buds of young shoots were used as explants. The shoots were multiplied in vitro on Murashige and Skoog (MS) supplemented with 0, 1.5, 2, and 2.5 mg.l −1 of N6benzyladenine (BA). The micropropagated/cloned shoots were rooted on MS and half strength (1/2) of MS containing indole-3-butyric acid (IBA) at three concentrations (0, 0.5, and 1 mg.l −1 ) and 0.5 mg.l −1 naphthalene acetic acid (NAA). The cultivar 'Malase Yazdi' multiplied better than 'Shirine Shahvar'. In both cultivars, the maximum shoot number with maximum shoot height was produced on medium with 2 mg.l −1 BA. Control medium free from plant growth regulator also supported shoot multiplication. The cultured shoots exhibited the greatest number of leaves on a medium containing 2.5 mg.l −1 BA. The cloned shoots of 'Malase Yazdi' rooted the best on half-strength MS medium with 0.5 mg.l −1 IBA while shoots of 'Shirine Shahvar', rooted on 1 mg.l −1 IBA and 0.5 mg.l −1 NAA in half MS medium. This method can be used to quickly propagate and conservation of these two pomegranate cultivars for future prospects.

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Section: Discussionmentioning

confidence: 99%