Pomegranate seed oil consumption during a period of high-fat feeding reduces weight gain and reduces type 2 diabetes risk in CD-1 mice

Abstract

The health benefits of pomegranate consumption have recently received considerable scientific focus, with most studies examining fruit and/or juice consumption. Pomegranate seed oil (POMo) is a rich source of 9-cis, 11-trans conjugate linolenic acid (CLA), which may offset the side-effects associated with weight gain. Male, wild-type CD-1 mice were divided into one of three groups (twenty per group): high-fat (HF), HF þ seed oil (HF þ POMo) or lean control (LN). In HF and HF þ POMo, mice were provided access ad libitum to a high-fat chow (60 % of energy from fat). HF þ POMo was supplemented with 61·79 mg POMo/d. LN consumed a restricted low-fat (10 % of energy from fat) chow to maintain body weight within 5 % of initial weight. Plasma was analysed for biomarkers associated with cholesterol profile (total cholesterol, HDL and TAG), glucose sensitivity (glucose and insulin), adipose tissue accumulation (leptin and adiponectin) and systemic low-grade inflammation (C-reactive protein and haptoglobin). The key findings of this study were that weight gain was associated with an increase in biomarkers of cholesterol profile, glucose sensitivity, adipose tissue accumulation and systemic low-grade inflammation (P,0·05). POMo only altered body weight accumulation, final body weight, leptin, adiponectin and insulin (P, 0·05). We found that despite a similar level of energy intake, HF mice had a greater concentration of leptin and a lower concentration of adiponectin compared to HF þ POMo mice. POMo intake was associated with an improvement in insulin sensitivity, suggesting that risk of developing type 2 diabetes may have been reduced; however, CVD risk did not change.High-fat feeding: Leptin: Adiponectin: Weight gain

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“…Up-regulation of PPAR-γ responsive genes by PA may be a mechanism of PSO-induced increase in serum insulin 79 . Anti-diabetic and insulin sensitizing effects of α-linolenic acid were also reported in other studies 53,[78][79][80][81] .…”

“…Insulin sensitivity was improved with 61.79mg/day PSO in mice which was treated with rich fat regimen compared to control group. In addition, final body weight, body weight accumulation, serum adiponectin, serum leptin, and serum insulin were reduced in treated group 78 .…”

Effect of dietary pomegranate seed oil and linseed oil on broiler chickens performance and meat fatty acid profile

“…It was stated that PSO reduced body weight gain, and leptin and insulin levels in pathogen-free mice (McFarlin et al, 2009). It was also observed that PSO supplementation in diet improves glucose tolerance and suppresses obesity related inflammation (Hontecillas et al, 2009).…”

Hatice Asli Bedel, Nur Tukel Turgut, Aysegul Ugur Kurtoglu, Coskun Usta

“…Recent investigations suggest that oral administration of PSO to mice with dietary PSO improved insulin resistance and high-fat-diet-induced obesity [38,39] . Vroegrijk et al also showed that body weights were decreased by reducing fat mass in PSO fed animals during 12 w. But they did not find insulin concentration or a lower fasted glucose in these animals [38] .…”