Pontirhabdus pectinivorans gen. nov., sp. nov., isolated from seawater

Abstract

A yellow-coloured, rod-shaped, Gram-reaction-negative and aerobic bacterial strain, designated JC2675T, was isolated from a seawater sample from Jeju Island, Korea. The isolate required sea salts for growth. Gliding motility was observed. Flexirubin-type pigments were absent. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain JC2675T represented a distinct phyletic line that reflected a novel generic status within the family Flavobacteraceae with relatively low gene sequence similarities (<95.7 %) to other recognized genera. The predominant isoprenoid quinone (MK-6) and DNA G+C content (30 mol%) were consistent with the assignment of the novel strain to the family Flavobacteriaceae, but overall phenotypic traits demonstrated that the novel strain was not closely affiliated with any previously described genus. Based on data from a study using a polyphasic taxonomic approach, it is proposed that strain JC2675T represents a new genus and novel species belonging to the family Flavobacteriaceae, for which the name Pontirhabdus pectinivorans gen. nov., sp. nov. is proposed. The type strain of the type species is JC2675T ( = KACC 14153T = JCM 17107T).

Algibacter amylolyticus sp. nov., isolated from intertidal sediment

“…Absorption spectra were recorded spectrophotometrically. The presence of carotenoids was verified by absorption peaks at 452 and 479 nm in the spectra of the acetone/methanol extracts of strain RU-4-M-4 T and A. pectinivorans KACC 14153 T , which is in agreement with data from Yi et al (2011).…”

“…The strain was routinely cultured on marine agar 2216 (MA; Difco) at 25 °C and stored as a suspension in skimmed milk (10 %, w/v) at −80 °C. Algibacter pectinivorans KACC 14153 T (Yi et al , 2011; Park et al , 2013c) was routinely grown on MA at 25 °C and used as reference strain.…”

Flavirhabdus iliipiscaria gen. nov., sp. nov., isolated from intestine of flounder (Paralichthys olivaceus) and emended descriptions of the genera Flavivirga, Algibacter, Bizionia and Formosa

“…The description of the genus is as given by Yi et al (2011) with the following amendments. Cells are catalase-negative.…”

“…Pairwise alignment according to the nearly complete 16S rRNA gene sequence (1441 bp) of strain Th68 T showed 96.1 % sequence similarity to the type strain of Pontirhabdus pectinivorans (Yi et al , 2011), which was later reclassified as Algibacter pectinivorans by Park et al (2013), followed by members of the genera Olleya (96.1–95.3 %), Flavivirga (96.1–95.1 %), Lacinutrix (96.0–95.0 %), Formosa (96.0–94.1 %), Arenitalea (95.4 %), Winogradskyella (95.4–93.4 %), Bizionia (95.1–94.1 %), Flaviramulus (95.0 %) and Gaetbulibacter (95.0–94.4 %). In the neighbour-joining (), maximum-likelihood and maximum-parsimony trees (Figs S1 and S2, available in the online Supplementary Material), the isolate formed a distinct phylogenetic branch within the family Flavobacteriaceae .…”

Algibacter agarivorans sp. nov. and Algibacter agarilyticus sp. nov., isolated from seawater, reclassification of Marinivirga aestuarii as Algibacter aestuarii comb. nov. and emended description of the genus Algibacter

“…The type species Algibacter lectus and the species Algibacter mikhailovii (Nedashkovskaya et al 2007) included strains from seaweed or strains that showed agarolytic activity. Recently, we reclassified a non-agarolytic marine bacterium Pontirhabdus pectinivorans (Yi et al, 2011) in this genus as Algibacter pectinivorans and described a novel species of the genus, Algibacter aquimarinus (Park et al, 2013b). During a study on the microbial diversity of the seawater of Gwangyang Bay, two yellow-pigmented flavobacterial strains, designated KYW560…”