Poor Thiamin and Riboflavin Status Is Common among Women of Childbearing Age in Rural and Urban Cambodia ,

Whitfield, Kyly C.; Karakochuk, Crystal D; Liu, Yazheng; McCann, Adrian; Talukder, Aminuzzaman; Kroeun, Hou; Ward, Mary; McNulty, Helene; Lynd, Larry D.; Kitts, David D.; Li‐Chan, Eunice C.Y.; McLean, J.; Green, Timothy

doi:10.3945/jn.114.203604

Cited by 49 publications

(39 citation statements)

References 36 publications

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“…Whitfield et al () reported that 82% of women ( n = 156) surveyed in Prey Veng, Cambodia, had biochemical evidence of low riboflavin status. Thus, interventions that result in a lower prevalence of inadequate riboflavin intake, as was found in the present study following intervention with EHFP plus aquaculture, are likely to improve nutrient status.…”

Section: Discussionmentioning

confidence: 99%

Effect of enhanced homestead food production and aquaculture on dietary intakes of women and children in rural Cambodia: A cluster randomized controlled trial

Verbowski

Talukder

Kroeun

et al. 2018

Maternal & Child Nutrition

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The Cambodian diet is low in nutrient-dense animal-source foods. Enhanced homestead food production (EHFP) and aquaculture, which increase availability of nutrient-dense foods, are promising interventions to improve dietary intake. This study examined the effect of EHFP with or without aquaculture on dietary intake and prevalence of inadequate intake of select nutrients among women and children living in rural Cambodia, compared to controls. In a registered, cluster randomized controlled trial in Prey Veng, Cambodia, 10 households in each of 90 villages (n = 900) were randomized by village to receive EHFP, EHFP plus aquaculture, or control. After 22-month intervention, 24-hr dietary recalls (24HRs) were collected from mothers aged 18-50 years (n = 429) and their children aged 6 months-7 years (n = 421), reported by their mothers. Usual intake distributions (generated using 24HRs and repeat 24HRs on a subsample) were used to estimate prevalence of inadequate intake. Compared to controls, women in the EHFP group had significantly higher zinc (+1.0 mg/d) and Vitamin A (+139 retinol activity equivalents/d) intakes, and women in the EHFP plus aquaculture group had significantly higher iron (+2.7 mg/d), Vitamin A (+191 retinol activity equivalents/d), and riboflavin (+0.17 mg/d) intakes. Women in the EHFP plus aquaculture group also had significantly lower prevalence of inadequate iron (-7%, at 10% bioavailability), Vitamin A (-19%), and riboflavin (-17%) intakes, compared to controls. No significant differences in intakes or nutrient adequacy were observed among children or between EHFP and EHFP plus aquaculture groups. The biological importance of the small differences in nutrient intakes among women remains to be established.

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Section: Discussionmentioning

confidence: 99%

Effect of enhanced homestead food production and aquaculture on dietary intakes of women and children in rural Cambodia: A cluster randomized controlled trial

Verbowski

Talukder

Kroeun

et al. 2018

Maternal & Child Nutrition

Self Cite

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“…Riboflavin deficiency is prevalent in many regions over the world, particularly in underdeveloped countries with a low intake of dairy products and meats5678. In the clinical conditions, patients with certain types of cancers and congenital heart disease, as well as those with excessive alcohol intake, are at a greater risk of riboflavin deficiency9.…”

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confidence: 99%

Riboflavin deficiency induces a significant change in proteomic profiles in HepG2 cells

Xin¹,

Li²,

Gao³

et al. 2017

Sci Rep

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Riboflavin deficiency is widespread in many regions over the world, especially in underdeveloped countries. In this study, we investigated the effects of riboflavin deficiency on protein expression profiles in HepG2 cells in order to provide molecular information for the abnormalities induced by riboflavin deficiency. HepG2 cells were cultured in media containing different concentrations of riboflavin. Changes of cell viability and apoptosis were assessed. A comparative proteomic analysis was performed using a label-free shotgun method with LC–MS/MS to investigate the global changes of proteomic profiles in response to riboflavin deficiency. Immunoblotting test was used to validate the results of proteomic approach. The cell viability and apoptosis tests showed that riboflavin was vital in maintaining the cytoactivity of HepG2 cells. The label-free proteomic analysis revealed that a total of 37 proteins showing differential expression (±2 fold, p < 0.05) were identified after riboflavin deficiency. Bioinformatics analysis indicated that the riboflavin deficiency caused an up-regulation of Parkinson’s disease pathway, steroid catabolism, endoplasmic reticulum stress and apoptotic process, while the fatty acid metabolism, tricarboxylic citrate cycle, oxidative phosphorylation and iron metabolism were down-regulated. These findings provide a molecular basis for the elucidation of the effects caused by riboflavin deficiency.

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“…A recent study in Cambodia found 50% of the women subjects in Phnom Penh and rural Cambodia were thiamine deficient (39% urban, 59% rural) [15]. In developed countries, thiamine deficiency and beriberi are rare on a population basis, but clinical deficiencies are reported worldwide [16].…”

Section: "Conflicts" Of Evidencementioning

confidence: 99%

Reconciling the Irreconcilable: Micronutrients in Clinical Nutrition and Public Health

J¹

2015

Vitam Miner

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Poor Thiamin and Riboflavin Status Is Common among Women of Childbearing Age in Rural and Urban Cambodia ,

Cited by 49 publications

References 36 publications

Effect of enhanced homestead food production and aquaculture on dietary intakes of women and children in rural Cambodia: A cluster randomized controlled trial

Effect of enhanced homestead food production and aquaculture on dietary intakes of women and children in rural Cambodia: A cluster randomized controlled trial

Riboflavin deficiency induces a significant change in proteomic profiles in HepG2 cells

Reconciling the Irreconcilable: Micronutrients in Clinical Nutrition and Public Health

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