Population Cytogenetic Evidence for Sibling Species in Anopheles annularis (Diptera: Culicidae)

Atrie, Bharati; Subbarao, S. K.; Pillai, M. K. K.; Rao, Satyawada Rama; Sharma, V. P.

doi:10.1093/aesa/92.2.243

Cited by 16 publications

(22 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we sequenced the rDNA ITS2 and D3 of A. annularis species collected from four different regions of India and found two types of sequences. Distribution of these two sequences among the specimens analyzed was similar to that reported by Atrie et al (1999) where they have shown the predominance of species A. They have also reported that in Assam, only species A was present, while both species A and B were present in Uttar Pradesh.…”

Section: Discussionsupporting

confidence: 85%

“…Similarly, we have found that majority of our specimens (161 of 169, i.e., 95.26%) contained one type of sequence, while the remaining 8 (4.73%) contained the other type (Table 1). Based on the distribution pattern and presence of species B in Ghaziabad (Uttar Pradesh), we have categorized our specimens in two categories as described by Atrie et al (1999). Molecular variations between two cryptic species of the Annularis complex were observed both at the ITS2 and D3 levels ( Figs.…”

Section: Discussionmentioning

confidence: 99%

“…In the states of Orissa, Assam, Rajasthan, and Haryana, only species A were found (Atrie et al 1999). Species A has been implicated in malaria transmission in Orissa and Assam but not in Rajasthan and Haryana (Atrie et al 1999). Species B has not been found to have its role in malaria transmission.…”

Section: Introductionmentioning

confidence: 91%

“…Although both species has sympatric distribution in Uttar Pradesh, none of them is a vector in the region. In the states of Orissa, Assam, Rajasthan, and Haryana, only species A were found (Atrie et al 1999). Species A has been implicated in malaria transmission in Orissa and Assam but not in Rajasthan and Haryana (Atrie et al 1999).…”

Section: Introductionmentioning

confidence: 94%

“…Recently, A. annularis in India were identified as a complex of two cryptic species based on the banding patterns of the ovarian polytene chromosomes and were provisionally designated as species A and B (Atrie et al 1999). Although both species has sympatric distribution in Uttar Pradesh, none of them is a vector in the region.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Identification of two cryptic species in the Anopheles (Cellia) annularis complex using ribosomal DNA PCR-RFLP

et al. 2006

View full text Add to dashboard Cite

Anopheles (Cellia) annularis Van der Wulp is a complex of two sibling species provisionally designated as species A and B and can only be differentiated on the basis of the paracentric inversion in the ovarian polytene chromosomes. To analyze the distribution of these two species and to develop a molecular method for the identification of these two cryptic species, we sequenced the ribosomal DNA internal transcribed spacer 2 (ITS2) and domain 3 (D3) of A. annularis specimens collected from Sonapur (Assam), Jabalpur (Madhya Pradesh), Ranchi (Jharkhand), and Ghaziabad (Uttar Pradesh). We did not find any sequence variation among the specimens collected from Assam, Madhya Pradesh, and Jharkhand states, whereas two types of sequences were obtained from the specimens collected from the state of Uttar Pradesh, which correspond to species A and B of the A. annularis complex. Species A was more prevalent among the all four regions studied. The ITS2 sequence of species A showed unique restriction sites for MvaI and Eco24I, while species B displayed HinfI and NruI sites. Similarly, the D3 sequence of species A showed unique restriction site for Alw26I, while species B showed a unique KpnI site. In this study, we report for the first time the development of ribosomal DNA polymerase chain reaction-restriction fragment length polymorphism methods for identifying these two cryptic species of the Annularis complex.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%