Population-dependent requirements of vitamin B12 and metabolically related substances of several mouse cell types in serum-free, albumin-fortified medium.

Matsuya, Yutaka; Yamane, Isao

doi:10.1247/csf.11.9

Cited by 5 publications

(1 citation statement)

References 28 publications

(7 reference statements)

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“…They explained that these metabolites, which the cells could synthesize in amount sufficient for sustained growth, but which at low population densities were lost from the cell to the environment in amount which exceeded the biosynthetic capacity of cells. Matsuya et al in 1986 also reported that the vitamin B 12 requirement of mouse FM3A cells was dependent on the population density [12]. The maximum viable cell number and integral of the number of viable cells over culture time were influenced by the serum concentration and the initial cell densities.…”

Section: Cell Culturesmentioning

confidence: 97%

Effect of Serum Concentration on Hybridoma Cell Growth and Monoclonal Antibody Production at Various Initial Cell Densities

Lee¹,

Huard²,

Palsson³

1989

Hybridoma

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Hybridoma cells (S3H5/gamma 2bA2) were cultivated using RPMI 1640 media with different serum concentrations at various initial cell densities. It was found that the serum requirement for cell growth was population-dependent. At an initial cell density of 1.10(4) cells/ml, the minimum serum concentration for cell growth was 5% (v/v) and reduced to 1% at the initial cell density of 1.10(5) cells/ml. When the initial density was increased to 1.10(6) cells/ml, serum was not needed for cell growth. The maximum cell density and the integral of viable cells over cultivation time increased as serum concentration or initial cell density was increased. However, specific antibody production rate was almost constant at 0.16 pg/cell.hr irrespective of serum concentration or initial cell density.

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Section: Cell Culturesmentioning

confidence: 97%

Effect of Serum Concentration on Hybridoma Cell Growth and Monoclonal Antibody Production at Various Initial Cell Densities

Lee¹,

Huard²,

Palsson³

1989

Hybridoma

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Best practices for media selection for mammalian cells

Price¹

2017

In Vitro Cell.Dev.Biol.-Animal

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Cell culture medium is a complex mixture of nutrients and growth factors that, along with the physical environment, can either help or destroy your experiment or production run. Nutritional requirements differ with different cell types and functions, as do optimal pH and osmolality. As cell growth proceeds, different cells will utilize amino acids and other components at different rates. By controlling for ammonia, free radicals, heavy metal toxicity, pH shifts, fluctuations in osmolality, nutrient depletion, and chemical and biological contaminants, you will optimize the chances of success. The contribution of each component of the medium is essential for the maintenance of the cell type of interest. While some cell types, such as established human cancer cell lines, may be quite able to tolerate a range of media and supplements, many normal cells and stems cells are not. Optimization of each component may be required to successfully maintain the latter cell types. The procedures for selecting and optimizing cell culture media and supplements are presented.

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Serum-free cell culture

Bjare¹

1992

Pharmacology & Therapeutics

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Population-dependent requirements of vitamin B12 and metabolically related substances of several mouse cell types in serum-free, albumin-fortified medium.

Abstract: ABSTRACT.Mouse FM3A cells propagated well in serum-free medium containing 0.5 % serum albumin and 1ƒÊg of insulin/ml. The vitamin B12 (B12)

Cited by 5 publications

References 28 publications

Effect of Serum Concentration on Hybridoma Cell Growth and Monoclonal Antibody Production at Various Initial Cell Densities

Effect of Serum Concentration on Hybridoma Cell Growth and Monoclonal Antibody Production at Various Initial Cell Densities

Best practices for media selection for mammalian cells

Serum-free cell culture

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