The Diarrhoeal Disease Surveillance System of icddr,b noted increasing number of patients ≥60 years at urban Dhaka and rural Matlab from 2001 to 2012. Shigella and Vibrio cholerae were more frequently isolated from elderly people than children under 5 years and adults aged 5-59 in both areas. The resistance observed to various drugs of Shigella in Dhaka and Matlab was trimethoprimsulphamethoxazole (72-63%), ampicillin (43-55%), nalidixic acid (58-61%), mecillinam (12-9%), azithromycin (13-0%), ciprofloxacin (11-13%) and ceftriaxone (11-0%). Vibrio cholerae isolated in Dhaka and Matlab was resistant to trimethoprim-sulphamethoxazole (98-94%), furazolidone (100%), erythromycin (71-53%), tetracycline (46-44%), ciprofloxacin (3-10%) and azithromycin (3-0%).keywords Bangladesh, diarrhoea, elderly, rural, urbanInnovations in medical science and evidence-based practices reduced case fatality and increased life expectancy globally (Stewart et al. 2013). This resulted in an increase in the proportions of people older than 60 years -a population with compromised immunity and other regulatory functions and at higher risk of chronic diseases such as diabetes mellitus, cardiorespiratory disorders and cerebrovascular diseases, especially in developing countries (Brown & Flood 2013 (Stoll et al. 1982). The DDSS in Dhaka systematically samples 2% of all patients irrespective of their age, sex, socio-demographic and economic status. The rural Matlab Hospital enrolled all patients coming from icddr,b Health and Demographic Surveillance System (HDSS) area. The DDSS was approved by both the Research Review Committee and the Ethical Review Committee of icddr,b, and verbal consent was provided by the patients or by parents/attending caregivers for minors. These two facilities provide cost-free care and treatment to more than 140 000 and 20 000 patients, respectively, each year (Das et al. 2013). We noted an increasing number of patients 60 years and older at both facilities ( Figure 1) and aimed to compare the proportion of patients in different age strata and the pattern of isolation of enteric pathogens among them from 2001 to 2012.The DDSS routinely collects clinical, epidemiological and laboratory information from enrolled patients, which include faecal culture for isolation and identification of major enteric pathogens, for example, Shigella, Vibrio cholerae, Campylobacter spp., enterotoxigenic Escherichia coli (ETEC), Entamoeba histolytica, Giardia lamblia and rotavirus. We compared the aetiology of diarrhoea in different age strata: <5, 5-59 and 60 years and older by chi-square test. A probability value of <0.05 was considered statistically significant. The magnitude of association was determined by estimating odds ratios (ORs) and their 95% confidence intervals (CIs).In Dhaka, the number of elderly patients has increased during the study period. In Matlab, the number of elderly individuals has been increasing over the period with reduced proportion of under-5 children, but there was no change among 5-to 59-year-old ad...