Population genetics and forensic efficiency data of 4 AMPFLP's

Abstract

Family studies were carried out in a population sample from north west Germany using 4 amplifiable VNTR polymorphic systems D1S80 (MCT118), ApoB, D17S30 (YNZ22) and COL2A1. Separation was carried out in polyacrylamide gels and visualised using silver staining. In family studies (n = 30) no evidence of new mutations was found. The population study of unrelated individuals (mothers and putative fathers) showed that all 4 systems were highly polymorphic and similar to other population studies. The combined exclusion chance was calculated to be approximately 99% and the combined discrimination index 1.5.10(-4). The Hardy-Weinberg equilibrium was checked by forming groups of alleles and no significant deviations could be found in all systems.

Genetic control of T cell receptor BJ gene expression in peripheral lymphocytes of normal and rheumatoid arthritis monozygotic twins.

“…Their ages ranged from 23-65 yr (mean age: 37.7). Monozygosity was confirmed by PCR amplification of genomic DNA with five pairs of oligonucleotide primers for variable number of tandem repeats (VNTR) (8) or by finger print analysis with anonymous probes. RA was diagnosed according to the American College of Rheumatology criteria (9).…”

Genetic Relationships among Japanese, Northern Han, Hui, Uygur, Kazakh, Greek, Saudi Arabian, and Italian Populations Based on Allelic Frequencies at Four VNTR (D1S80, D4S43, COL2A1, D17S5) and One STR (ACTBP2) Loci

“…The PCR primers and reaction conditions for amplification of the D1S80, D4S43, D17S5, COL2A1, and ACTBP2 loci have been described previously [5, 6, 7, 9, 10]. For amplification of the D4S43, COL2A1, and ACTBP2 loci, the forward or reverse primer was labeled at the 5′ end with a fluorescent dye, 6-carboxyfluorescein or hexachlorinated or tetrachlorinated analogue.…”

A new sequenced allelic ladder marker for D1S80 typing

“…The D1S80 locus is very useful for human identification because of its high polymorphism, especially in the Japanese population (Rand et al 1992;Sugiyama et al 1993;TWGFDM 1995). Baechtel et al (1993) described an allelic ladder marker that consisted of alleles 16-37 and 41 of the locus, but they were not sequenced.…”