Population genetics of Sargassum horneri (Fucales, Phaeophyta) in China revealed by ISSR and SRAP markers

Yu, Shenhui; Chong, Zhuo; Zhao, Fangming; Yao, Jianting; Duan, Delin

doi:10.1007/s00343-013-2064-8

Cited by 7 publications

(4 citation statements)

References 26 publications

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“…Changes in bands may be the consequences of genomic template instability (GTS) related to the level of DNA damage, the efficiency of DNA repair and replication (Atienzar et al, 2002;Atienzar andJha, 2004 andCiğerci et al, 2016). Yu et al (2013) reported that high genetic variability and differentiation existed among Sargassum horneri populations using ISSR and RAPD. They also reported that the genetic differentiation of S. horneri populations along China's coast agreed with the geographic isolation, but some populations are genetically closer than others, even when these populations are widely separated geographically.…”

Section: Figure 2 Dendogram For (1) Dictyota Dichotoma Collected Fromentioning

confidence: 99%

Impact of Different Geographical Locations on Genetic Variation and Phytochemical Constituents of Two Medicinal Marine Algae.

Saleh

El-Atroush

2020

Taeckholmia

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Genetic variations and phytochemical constituents of Dictyota dichotoma and Sargassum dentifolium, collected from Hurghada and Abu-Qir bay, were investigated. Results revealed an increase in the concentrations of carotenoids, sugars and DPPH% in the samples of both algal species collected from Hurghada as compared with those collected from Abu-Qir. Regarding the samples collected from Hurghada, an increase in the concentrations of chlorophyll a, b and phenols was recorded in S. dentifolium; while a decrease in chlorophyll a, b only was observed in D. dichotoma. No significant difference was observed in phenol concentrations between D. dichotoma samples collected from the two studied localities. DNA was isolated and thirteen SCoT molecular markers were used to detect the genetic variations between the samples of the two localities. In D. dichotoma, the total average of polymorphism is 56.68%. SCoT-1 exhibited the highest polymorphism (80%), while SCoT-28 recorded the lowest polymorphism (35.71%). In S. dentifolium, the total average of polymorphism is 61.63%. SCoT-3 showed the highest polymorphism (90%), while SCoT-13 exhibited the lowest polymorphism (0.09%). In addition, BSI factor showed a marked difference between the two studied algae that it was 0.87 % in D. dichotoma and 0.77 % in S. dentifolium. Variation in geographical location affects genetic variation and phytochemical composition of D. dichotoma and S. dentifolium. The difference in the responses of the two studied algae to the change of locality and environmental conditions may reflect differences in their gene expression. Moreover, the efficiency of SCoT markers to discriminate between algal species collected from different localities has been confirmed.

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Section: Figure 2 Dendogram For (1) Dictyota Dichotoma Collected Fromentioning

confidence: 99%

Impact of Different Geographical Locations on Genetic Variation and Phytochemical Constituents of Two Medicinal Marine Algae.

Saleh

El-Atroush

2020

Taeckholmia

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“…Theoretically, populations 2 and 3 originated from the same area; therefore, they should have similar genetic variation, but our results did not support this assumption. Yu et al (2013) reported that the genetic differentiation of the S. horneri populations along China's coast agreed with the geographic isolation, but some populations are genetically closer than others, even when these populations are widely separated geographically. The cluster results of our study also showed the closed genetic distance between populations 1, 2, and 5, which originated broadly from the north to south.…”

Section: Discussionmentioning

confidence: 81%

“…Similar results were obtained in studies on Sargassum species in Oman Sea by Noormohammadi et al (2011). Yu et al (2013) reported that high genetic variability and differentiation existed among S. horneri populations: the inter-population P% was 99.4 and 100.0% by ISSR and RAPD, respectively. In the same study, the highest P% within a population was detected in the YT and NJ populations (50%).…”

Section: Discussionmentioning

confidence: 99%

Genetic variation of Sargassum horneri populations detected by inter-simple sequence repeats

Ren

Yang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The seaweed Sargassum horneri is an important brown alga in the marine environment, and it is an important raw material in the alginate industry. Unfortunately, the fixed resource that was originally reported is now reduced or disappeared, and increased floating populations have been reported in recent years. We sampled a floating population and 4 fixed cultivated populations of S. horneri along the coast of Zhejiang, China. Inter-simple sequence repeat (ISSR) markers were applied in this research to analyze the genetic variation between floating populations and fixed cultivated populations of S. horneri. In total, 220 loci were amplified with 23 ISSR primers. The percentage of polymorphic loci within each population ranged from 53.64 to 95.45%. The highest diversity was observed in population 3, which was the local species that was suspension cultured in the lab and then fixed cultivated in the Nanji Islands before sampling. The lowest diversity was obtained in the floating population 4. The genetic distances among 620 J.R. Ren et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 619-625 (2015) the 5 S. horneri populations ranged from 0.0819 to 0.2889, and the distance tendency confirmed the genetic diversity. The results suggest that the floating population had the lowest genetic diversity and could not be joined into the cluster branch of the fixed cultivated populations.

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“…Therefore, S. horneri has attracted considerable attentions. However, most studies of S. horneri have been focused on its medicinal value , population genetics , and nutritional evaluation . To the best of our knowledge, little research has been carried out to investigate the phytohormone composition and distribution in S. horneri to assess its current state and sustainable development.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of ten phytohormones in Sargassum horneri by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry

Zhou

Yan

et al. 2016

J of Separation Science

107

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Phytohormones have attracted wide attention due to their important biological functions. However, their detection is still a challenge because of their complex composition, low abundance and diverse sources. In this study, a novel method of high-performance liquid chromatography with electrospray ionization tandem mass spectrometry was developed and validated for the simultaneous determination of ten phytohormones including indole-3-acetic acid, isopentenyladenine, isopentenyl adenosine, trans-zeatin riboside, zeatin, strigolactones, abscisic acid, salicylic acid, gibberellin A3, and jasmonic acid in Sargassum horneri (S. horneri). The phytohormones were extracted from freeze-dried S. horneri with methanol/water/methanoic acid (15:4:1, v/v/v) analyzed on a Hypersil Gold C18 column and detected by electrospray ionization tandem triple quadrupole mass spectrometry in the multiple reaction monitoring mode. The experimental conditions for the extraction and analysis of phytohormones were optimized and validated in terms of reproducibility, linearity, sensitivity, recovery, accuracy, and stability. Distributions of the phytohormones in the stems, blades, and gas bladder of the S. horneri in drift, fixed, and semi-fixed growing states were investigated for the first time. The observed contents of the phytohormones in S. horneri range from not detected to 5066.67 ng/g (fresh weight). Most phytohormones are distributed mainly in the stems of S. horneri in drift and semi-fixed states.

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Population genetics of Sargassum horneri (Fucales, Phaeophyta) in China revealed by ISSR and SRAP markers

Cited by 7 publications

References 26 publications

Impact of Different Geographical Locations on Genetic Variation and Phytochemical Constituents of Two Medicinal Marine Algae.

Impact of Different Geographical Locations on Genetic Variation and Phytochemical Constituents of Two Medicinal Marine Algae.

Genetic variation of Sargassum horneri populations detected by inter-simple sequence repeats

Simultaneous analysis of ten phytohormones in Sargassum horneri by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry

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