Population Structure of
            <i>Staphylococcus aureus</i>
            Strains Isolated from Intensive Care Unit Patients in The Netherlands over an 11-Year Period (1996 to 2006)

Rijnders, Marlies; Deurenberg, Ruud H.; Boumans, Marie-Louise; Hoogkamp-Korstanje, J. A. A.; Beisser, Patrick S.; Stobberingh, Ellen E.

doi:10.1128/jcm.00820-09

Cited by 41 publications

(27 citation statements)

References 55 publications

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“…However, the other spa types t3992, t1200, t2770, t311, t2104, t2478, t2802, t5019, t2915, and t6861 of Staph. aureus reported in this study from bovine milk were earlier reported from sources other than bovine milk (Rijnders et al 2009;Gomez-Sanz et al 2010;Deurenberg et al 2010;http://spaserver.ridom.de/). This emphasizes that there is a need to understand the pathogenic role and significance of these strains in bovine mastitis.…”

Section: Discussionsupporting

confidence: 53%

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Staphylococcus aureus spatype t267, clonal ancestor of bovine subclinical mastitis in India

et al. 2013

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Aim: To evaluate the virulence determinants and genetic diversity of Staphylococcus aureus from bovine subclinical mastitis milk. Methods and Results: PCR detection of virulence genes was performed for 173 Staph. aureus from bovine subclinical mastitis milk. Further, genetic diversity was analysed by agr and spa typing followed by pulsed field gel electrophoresis (PFGE) of selected isolates. Screening of virulence genes (n = 19) showed the adherence genes viz. fnbA, clfA, fnbB and cna in 98Á8, 97Á1, 68Á8 and 28Á3 percentage of isolates, respectively, and 80 strains (46Á24%) positive for enterotoxin genes were distributed as 23 toxinotypes, of which, 5 genotypes contained a single gene and the rest comprised of multiple toxin genes. Out of agr type-1 (87Á3%), 74Á2 per cent belonged to the three predominant spa types. Of 27 spa types, 11 were identified for the first time. The predominant spa types were t267 (N =44), t359 (N = 42) and t6877 (N =29), which together accounts to 66Á5 per cent of isolates. PFGE analysis of isolates (N = 45) covering all the spa types revealed mostly similar or closely related pulsotypes. Local emergence of spa type t6877 in herd-dependant manner was observed. spa sequence-based phylogenetic analysis suggested t267 as the ancestral clone of t359, t6877 and other spa types except two. Conclusion: Heterogenous virulence profile of the isolates had no significant association with the genotype. High prevalence of agr group I reaffirms their association with persistent subclinical mastitis. The spa type t267 appears to be the ancestral clone endemic in the region causing subclinical mastitis. In addition, few new spa types have emerged in the geographic region. Significance and Impact of Study: Gives an insight into the genetic and evolutionary behaviour of Staph. aureus associated with bovine subclinical mastitis in India. The study would pave the way for devising effective control strategy for bovine mastitis in Indian context.

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Section: Discussionsupporting

confidence: 53%

“…aureus reported in this study from bovine milk were earlier reported from sources other than bovine milk (Rijnders et al . ; Gomez‐Sanz et al . ; Deurenberg et al .…”

Section: Discussionmentioning

confidence: 99%

Staphylococcus aureus spatype t267, clonal ancestor of bovine subclinical mastitis in India

et al. 2013

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“…Although the prevalence of MSSA with the genetic background common to MRSA (CC45, CC5, CC8, CC22) in the hospital was lower than in other studies [20][21][22][23], the MRSA prevalence among inpatient S. aureus was relatively high. At the same time, the number of MSSA with the genetic background common to MRSA (CC45, CC5, CC8, CC22, and CC30) in outpatients was higher than in hospitalised patients, but the prevalence of MRSA was much lower in this setting when compared with the hospital.…”

Section: Discussioncontrasting

confidence: 60%

Molecular characterisation of methicillin-susceptible and methicillin-resistant Staphylococcus aureus in inpatients and outpatients in Bosnia and Herzegovina

Uzunović-Kamberović

Rijnders

Stobberingh

et al. 2012

Wien Med Wochenschr

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The aim of this study was to investigate the genetic background of methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) obtained from clinical specimens of inpatients and outpatients. Methicillin resistance was confirmed by the presence of the mecA gene by PCR. The genetic characterisation was performed using spa typing and the algorithm based upon repeat pattern (BURP). Staphylococcus aureus was isolated from 68 and 79 inpatient and outpatient samples, 31 (46 %) and 14 (18 %) of which were MRSA, respectively. Among 37 inpatients and 65 outpatients with MSSA, 22 and 38 spa types were clustered into seven and eight spa-CCs, respectively. The main MSSA spa-CC of inpatients and outpatients was spa-CC015 (multilocus sequence typing (MLST) CC45). Most MRSA were associated with spa-CC355/595 (MLST CC152). MRSA-related background was found in 32 % of inpatients and 43 % of outpatients with MSSA, suggesting that MRSA did not arise from predominant MSSA clones.

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“…Interestingly, patterns of IgG4 response varied extensively between volunteers, indicating that each person is exposed to different virulence factors and/or reacts differently. The different exposure to virulence factors could be explained by the fact that various genetic backgrounds of S. aureus contain different sets of virulence factors, and variation may also be due to differences in regulators or gene expression in various strains (24,29,(65)(66)(67)(68)(69).…”

Section: Discussionmentioning

confidence: 99%

IgG4 Subclass-Specific Responses to Staphylococcus aureus Antigens Shed New Light on Host-Pathogen Interaction

et al. 2015

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f IgG4 responses are considered indicative for long-term or repeated exposure to particular antigens. Therefore, studying IgG4-specific antibody responses against Staphylococcus aureus might generate new insights into the respective host-pathogen interactions and the microbial virulence factors involved. Using a bead-based flow cytometry assay, we determined total IgG (IgGt), IgG1, and IgG4 antibody responses to 40 different S. aureus virulence factors in sera from healthy persistent nasal carriers, healthy persistent noncarriers, and patients with various staphylococcal infections from three distinct countries. IgGt responses were detected against all tested antigens. These were mostly IgG1 responses. In contrast, IgG4 antibodies were detected to alphatoxin, chemotaxis inhibitory protein of S. aureus (CHIPS), exfoliative toxins A and B (ETA and -B), HlgB, IsdA, LukD, -E, -F, and -S, staphylococcal complement inhibitor (SCIN), staphylococcal enterotoxin C (SEC), staphylococcal superantigen-like proteins 1, 3, 5, and 9 (SSL1, -3, -5, and -9), and toxic shock syndrome toxin 1 (TSST-1) only. Large interpatient variability was observed, and the type of infection or geographical location did not reveal conserved patterns of response. As persistent S. aureus carriers trended toward IgG4 responses to a larger number of antigens than persistent noncarriers, we also investigated sera from patients with epidermolysis bullosa (EB), a genetic blistering disease associated with high S. aureus carriage rates. EB patients responded immunologically to significantly more antigens than noncarriers and trended toward even more responses than carriers. Altogether, we conclude that the IgG4 responses against a restricted panel of staphylococcal antigens consisting primarily of immune modulators and particular toxins indicate important roles for these virulence factors in staphylococcal pathogen-host interactions, such as chronicity of colonization and/or (subclinical) infections.

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Population Structure of Staphylococcus aureus Strains Isolated from Intensive Care Unit Patients in The Netherlands over an 11-Year Period (1996 to 2006)

Cited by 41 publications

References 55 publications

Staphylococcus aureus spatype t267, clonal ancestor of bovine subclinical mastitis in India

Staphylococcus aureus spatype t267, clonal ancestor of bovine subclinical mastitis in India

Molecular characterisation of methicillin-susceptible and methicillin-resistant Staphylococcus aureus in inpatients and outpatients in Bosnia and Herzegovina

IgG4 Subclass-Specific Responses to Staphylococcus aureus Antigens Shed New Light on Host-Pathogen Interaction

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